Pesticidal genes and methods of use

ABSTRACT

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest, including microorganisms and plants. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and using those polypeptides for controlling or killing a pest.

CROSS REFERENCE TO RELATED APPLICATIONS

The present application is a divisional application of U.S. applicationSer. No. 17/009,339, filed Sep. 1, 2020, which is a divisionalapplication of U.S. application Ser. No. 15/882,467, filed Jan. 29,2018, now U.S. Pat. No. 10,793,610, issued Oct. 6, 2020, which claimsthe benefit of U.S. Provisional Patent Application No. 62/451,914, filedJan. 30, 2017, and U.S. Provisional Application No. 62/476,918, filedMar. 27, 2017, and U.S. Provisional Application No. 62/543,545, filedAug. 10, 2017, the disclosures of which are incorporated herein byreference in their entirety.

FIELD OF THE INVENTION

The invention is drawn to methods and compositions for controllingpests, particularly plant pests.

SEQUENCE LISTING

The official copy of the sequence listing is submitted electronicallyvia Patent Center as an XML formatted sequence listing with a file namedAGB045USDIV2SEQLIST_Corrected.xml, created on Feb. 2, 2023, and having asize of 712 KB. The sequence listing contained in this XML formatteddocument is part of the specification and is herein incorporated byreference in its entirety.

BACKGROUND

Pests, plant diseases, and weeds can be serious threats to crops. Lossesdue to pests and diseases have been estimated at 37% of the agriculturalproduction worldwide, with 13% due to insects, bacteria and otherorganisms.

Toxins are virulence determinants that play an important role inmicrobial pathogenicity and/or evasion of the host immune response.Toxins from the gram-positive bacterium Bacillus, particularly Bacillusthuringiensis, have been used as insecticidal proteins. Currentstrategies use the genes expressing these toxins to produce transgeniccrops. Transgenic crops expressing insecticidal protein toxins are usedto combat crop damage from insects.

While the use of Bacillus toxins has been successful in controllinginsects, resistance to Bt toxins has developed in some target pests inmany parts of the world where such toxins have been used intensively.One way of solving this problem is sowing Bt crops with alternating rowsof regular non Bt crops (refuge). An alternative method to avoid or slowdown development of insect resistance is stacking insecticidal geneswith different modes of action against insects in transgenic plants. Thecurrent strategy of using transgenic crops expressing insecticidalprotein toxins is placing increasing emphasis on the discovery of noveltoxins, beyond those already derived from the bacterium Bacillusthuringiensis. These toxins may prove useful as alternatives to thosederived from B. thuringiensis for deployment in insect- andpest-resistant transgenic plants. Thus, new toxin proteins are needed.

SUMMARY

Compositions having pesticidal activity and methods for their use areprovided. Compositions include isolated and recombinant polypeptidesequences having pesticidal activity, recombinant and synthetic nucleicacid molecules encoding the pesticidal polypeptides, DNA constructscomprising the nucleic acid molecules, vectors comprising the nucleicacid molecules, host cells comprising the vectors, and antibodies to thepesticidal polypeptides. Nucleotide sequences encoding the polypeptidesprovided herein can be used in DNA constructs or expression cassettesfor transformation and expression in organisms of interest, includingmicroorganisms and plants.

The compositions and methods provided herein are useful for theproduction of organisms with enhanced pest resistance or tolerance.These organisms and compositions comprising the organisms are desirablefor agricultural purposes. Transgenic plants and seeds comprising anucleotide sequence that encodes a pesticidal protein of the inventionare also provided. Such plants are resistant to insects and other pests.

Methods are provided for producing the various polypeptides disclosedherein, and for using those polypeptides for controlling or killing apest. Methods and kits for detecting polypeptides of the invention in asample are also included.

DETAILED DESCRIPTION OF THE INVENTION

The present inventions now will be described more fully hereinafter withreference to the accompanying drawings, in which some, but not allembodiments of the inventions are shown. Indeed, these inventions may beembodied in many different forms and should not be construed as limitedto the embodiments set forth herein; rather, these embodiments areprovided so that this disclosure will satisfy applicable legalrequirements. Like numbers refer to like elements throughout.

Many modifications and other embodiments of the inventions set forthherein will come to mind to one skilled in the art to which theseinventions pertain having the benefit of the teachings presented in theforegoing descriptions and the associated drawings. Therefore, it is tobe understood that the inventions are not to be limited to the specificembodiments disclosed and that modifications and other embodiments areintended to be included within the scope of the appended claims.Although specific terms are employed herein, they are used in a genericand descriptive sense only and not for purposes of limitation.

I. Polynucleotides and Polypeptides

Compositions and method for conferring pesticidal activity to anorganism are provided. The modified organism exhibits pesticidalresistance or tolerance. Recombinant pesticidal proteins, orpolypeptides and fragments and variants thereof that retain pesticidalactivity, are provided and include those set forth in SEQ ID NOs: 1, 2,3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40,41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58,59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76,77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94,95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109,110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123,124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151,152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165,166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193,194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207,208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221,222, 223, 224, 225, 226, 227, 228, 229,230, 231, 232, 233, 234, 235,236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249,250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263,264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277,278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291,292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305,306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319,320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333,334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347,348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375,376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389,390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403,404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417,418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431,432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445,446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, and/or 458.The pesticidal proteins are biologically active (e.g., pesticidal)against pests including insects, fungi, nematodes, and the like.Nucleotides encoding the pesticidal polypeptides, including for example,SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71,72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105,106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119,120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133,134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217,218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245,246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259,260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273,274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287,288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301,302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315,316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329,330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343,344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385,386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399,400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427,428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441,442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455,456, 457, and/or 458 or active fragments or variants thereof, can beused to produce transgenic organisms, such as plants and microorganisms.The pesticidal proteins are biologically active (for example, arepesticidal) against pests including insects, fungi, nematodes, and thelike. In specific embodiments, the pesticidal polypeptides and theactive variant and fragments thereof have an improved pesticidalactivity when compared to other polypeptides in the art. Polynucleotidesencoding the pesticidal polypeptides, including for example, SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20,21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56,57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74,75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108,109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122,123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164,165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178,179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206,207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220,221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234,235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248,249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262,263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276,277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290,291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304,305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346,347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360,361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388,389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402,403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416,417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430,431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444,445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, and/or458 or active fragments or variants thereof, can be used to producetransgenic organisms, such as plants and microorganisms. The transformedorganisms are characterized by genomes that comprise at least one stablyincorporated DNA construct comprising a coding sequence for a pesticidalprotein disclosed herein. In some embodiments, the coding sequence isoperably linked to a promoter that drives expression of the encodedpesticidal polypeptide. Accordingly, transformed microorganisms, plantcells, plant tissues, plants, seeds, and plant parts are provided. Asummary of various polypeptides, active variants and fragments thereof,and polynucleotides encoding the same are set forth below in Table 1. Asnoted in Table 1, various forms of polypeptides are provided. Fulllength pesticidal polypeptides, as well as, modified versions of theoriginal full-length sequence (i.e., variants) are provided. Table 1further denotes “CryBP1” sequences. Such sequences (SEQ ID NOS: 213,227, and 323) comprise accessory polypeptides that can be associatedwith some of the toxin genes. In such instances, the CryBP1 sequencescan be used alone or in combination with any of the pesticidalpolypeptides provided herein. Table 1 further provides Split-CryC-terminus polypeptides (SEQ ID NO: 131 and 201). Such sequencecomprises the sequence of a downstream protein that has homology to theC-terminal end of the Cry class of toxin genes and are usually foundafter a Cry gene that is not full-length and is missing the expectedC-terminal region.

TABLE 1 Summary of SEQ ID NOs, Gene Class, and Variants thereof Full-Split-Cry length Modified CryBP1 C-terminus SEQ ID SEQ ID SEQ ID SEQ IDIdentity from Similarity from Gene Name No. No.(s) No. No. Gene Classnearest non-APG nearest non-APG Homologs APG00929 1 Bin 55, 60, 65, 70,75, 80, 70, 75, 80, 85, 90, 95, WP_028278541.1 (51.83% identity, 65.75%85, 90, 95, 96, 97, 98, 96, 97, 98, 99 similarity) 99 WP_012623447.1(48.40% identity, 62.10% similarity) WP_026555044.1 (44.77% identity,58.41% similarity) WP_055800425.1 (44.42% identity, 58.09% similarity)APG00954 2 MTX 70, 75, 80, 85, 90, 95, 85, 90, 95, 96, 97, 98,APG00408 - US_2016_0311864_A1-171 (95.97% 96, 97, 98, 99 99 identity,98.66% similarity) APG00067 - US_2016_0311864_A1-7 (82.27% identity,89.30% similarity) APG00559 - US_2016_0311864_A1-191 (72.91% identity,80.60% similarity) APG08589 (70.23% identity, 82.61% similarity)US_2013_0227743_A1-100 (69.90% identity, 81.27% similarity) APG08088(69.77% identity, 81.40% similarity) APG06690 (69.08% identity, 81.25%similarity) APG04720 (68.77% identity, 79.73% similarity) APG00201 -US_2016_0304898_A1-193 (68.63% identity, 78.76% similarity) APG00006 -US_2016_0304898_A1-9 (68.3% identity, 80.07% similarity) APG01044 3  4MTX 30, 35, 40, 45, 50, 55, 50, 55, 60, 65, 70, 75, APG00148 -US_2016_0304898_A1-170 (33.61% 60, 65, 70, 75, 80, 85, 80, 85, 90, 95,96, 97, identity, 51.54% similarity) 90, 95, 96, 97, 98, 99 98, 99US_2013_0227743_A1-112 (29.81% identity, 45.80% similarity)WP_065845806.1 (28.41% identity, 46.67% similarity) ADK08315.1 (27.50%identity, 43.89% similarity) APG01087 5  6 MTX 45, 50, 55, 60, 65, 70,65, 70, 75, 80, 85, 90, WP_006918908.1 (43.44% identity, 64.38% 75, 80,85, 90, 95, 96, 95, 96, 97, 98, 99 similarity) 97, 98, 99 WP_065845806.1(40.37% identity, 60.24% similarity) SDW46809.1 (33.15% identity, 50.97%similarity) SDX17518.1 (31.73% identity, 50.71% similarity) APG01231 7 8 MTX 95, 96, 97, 98, 99 95, 96, 97, 98, 99 APG00704 (93.66% identity,95.59% similarity) WP_071770709.1 (93.66% identity, 94.77% similarity)APG00020 - US_2016_0304898_A1-33 (82.42% identity, 89.01% similarity)APG00253 (82.14% identity, 87.91% similarity) APG00635.1 -US_2016_0311864_A1-369 (81.32% identity, 87.64% similarity) APG00764(79.58% identity, 84.03% similarity) APG00268 (79.06% identity, 84.55%similarity) APG08278 (78.80% identity, 84.29% similarity) APG00635 -US_2016_0311864_A1-201 (77.49% identity, 83.51% similarity) APG01309 9Cyt 30, 35, 40, 45, 50, 55, 40, 45, 50, 55, 60, 65, APG00464 (97.56%identity, 98.54% similarity) 60, 65, 70, 75, 80, 85, 70, 75, 80, 85, 90,95, APG00017 - US_2016_0304898_A1-28 (95.61% 90, 95, 96, 97, 98, 99 96,97, 98, 99 identity, 98.05% similarity) APG00194.1 -US_2016_0311864_A1-251 (63.59% identity, 78.16% similarity) APG00194 -US_2016_0311864_A1-65 (58.74% identity, 72.2% similarity) Cyt2Ca1(29.84% identity, 39.92% similarity) APG01329 10 11, 12 Cry32 95, 96,97, 98, 99 96, 97, 98, 99 US_2011_0203014-23 (93.27% identity, 95.57%similarity) U5KRS1_BACTU (93.2% identity, 95.57% similarity) AGA40054.1(89.94% identity, 93.52% similarity) APG00710 (86.69% identity, 90.7%similarity) APG07870 (82.97% identity, 88.14% similarity) APG00430(77.24% identity, 83.52% similarity) APG00058 - US_2016_0311864_A1-3(73.34% identity, 80.74% similarity) APG00056 - US_2016_0311864_A1-1(69.12% identity, 79.80% similarity) APG01387 (66.29% identity, 75.32%similarity) APG00687 (57.53% identity, 67.25% similarity) APG03519(55.50% identity, 67.16% similarity) Cry32Ab1 (53.97% identity, 65.37%similarity) APG05370 (53.33% identity, 64.47% similarity) APG01387 1314, 15 Cry32 70, 75, 80, 85, 90, 95, 80, 85, 90, 95, 96, 97, APG00058 -US_2016_0311864_A1-3 (89.02% 96, 97, 98, 99 98, 99 identity, 90.75%similarity) APG07870 (74.81% identity, 81.03% similarity) APG00710(68.39% identity, 77.05% similarity) APG00430 (67.97% identity, 77.71%similarity) U5KRS1_BACTU (67.82% identity, 76.13% similarity)US_2011_0203014-23 (67.82% identity, 76.13% similarity) APG01329 (66.29%identity, 75.32% similarity) APG00056 - US_2016_0311864_A1-1 (64.84%identity, 75.78% similarity) APG00469 (54.20% identity, 65.84%similarity) Cry32Da1 (53.60% identity, 65.70% similarity) APG05370(53.31% identity, 63.77% similarity) APG03519 (53.17% identity, 65.72%similarity) APG01399 16  17 Cry 99 100 WP_017762619.1 (98.64% identity,99.66% similarity) US_2013_0227743_A1-206 (55.95% identity, 68.51%similarity) APG00460 (51.63% identity, 65.36% similarity) WP_017762581.1(34.10% identity, 47.84% similarity) WP_044306759.1 (33.65% identity,48.41% similarity) APG01459 18  19 Cry70 95, 96, 97, 98, 99 97, 98, 99APG00728 - US_2016_0304898_A1-219 (98.39% identity, 99.13% similarity)APG00728.2 - US_2016_0304898_A1-221 (94.80% identity, 95.42% similarity)APG00025 - US_2016_0304898_A1-41 (94.06% identity, 96.78% similarity)K0G027_BACTU (93.32% identity, 96.29% similarity) APG00526 -US_2016_0304898_A1-214 (93.07% identity, 95.30% similarity) Cry70Ba1(92.95% identity, 96.04% similarity) APG00705 (84.33% identity, 91.06%similarity) APG00595 (65.56% identity, 76.84% similarity) APG01474 20 21 Vip4 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 95,US_2013_0227743_A1-130 (53.35% identity, 68.76% 85, 90, 95, 96, 97, 98,96, 97, 98, 99 similarity) 99 WP_016123960.1 (53.06% identity, 67.30%similarity) AGT29561.1 (50.61% identity, 67.37% similarity) Vip4Aa1(49.80% identity, 68.60% similarity) APG01506 22 MTX 80, 85, 90, 95, 96,97, 90, 95, 96, 97, 98, 99 APG07655 (81.42% identity, 88.18% similarity)98, 99 APG03379 (81.21% identity, 87.58% similarity) APG00155 -US_2016_0311864_A1-40 (80.74% identity, 88.18% similarity) APG00201.1 -US_2016_0304898_A1-194 (80.47% identity, 86.87% similarity) APG08589(80.41% identity, 90.20% similarity) APG08241 (80.07% identity, 88.18%similarity) US_2013_0227743_A1-100 (79.73% identity, 88.18% similarity)J8YPM2_BACCE (79.05% identity, 88.18% similarity) APG00107 -US_2016_0311864_A1-21 (78.72% identity, 88.18% similarity) APG00201 -US_2016_0304898_A1-193 (78.62% identity, 84.87% similarity) APG09682(77.78% identity, 86.87% similarity) APG00847 - US_2016_0304898_A1-223(77.63% identity, 84.54% similarity) APG01517 23  24 MTX 40, 45, 50, 55,60, 65, 60, 65, 70, 75, 80, 85, APG03732 (95.22% identity, 97.91%similarity) 70, 75, 80, 85, 90, 95, 90, 95, 96, 97, 98, 99US_2013_0227743_A1-114 (36.09% identity, 55.10% 96, 97, 98, 99similarity) US_2013_0227743_A1-112 (34.44% identity, 55.10% similarity)KOS27986.1 (24.23% identity, 41.78% similarity) WP_000052062.1 (23.96%identity, 41.78% similarity) APG01522 25  26 Cry34 99 99 WP_069325540.1(98.29% identity, 98.29% similarity) APG02291 (90.60% identity, 94.02%similarity) WP_046215736.1 (87.18% identity, 92.31% similarity)WP_052520572.1 (59.83% identity, 76.07% similarity) Cry34Ab1 (46.03%identity, 57.94% similarity) APG01644 27  28 Cry 25, 30, 35, 40, 45, 50,40, 45, 50, 55, 60, 65, APG00110 - US_2016_0177333_A1-60 (86.86% 55, 60,65, 70, 75, 80, 70, 75, 80, 85, 90, 95, identity, 93.02% similarity) 85,90, 95, 96, 97, 98, 96, 97, 98, 99 APG00110.1 - US_2016_0177333_A1-61(83.26% 99 identity, 89.42% similarity) APG00110.3 -US_2016_0177333_A1-62 (66.05% identity, 69.88% similarity) APG00672(62.61% identity, 74.0% similarity) APG00045 - US_2016_0304898_A1-73(62.56% identity, 73.9% similarity) APG01742 (61.25% identity, 74.02%similarity) Cry70Bb1 (22.81% identity, 37.38% similarity) APG01676 29 30 MTX 95, 96, 97, 98, 99 97, 98, 99 AOB42285.1 (93.31% identity,96.66% similarity) APG08411 (86.63% identity, 92.10% similarity)APG00989 (86.63% identity, 91.79% similarity) APG00309 (82.88% identity,89.49% similarity) C3HSG6_BACTU (70.30% identity, 82.73% similarity)WP_018673409.1 (67.66% identity, 78.74% similarity) APG00585 (66.47%identity, 77.25% similarity) APG00427 - US_2016_0311864_A1-178 (64.55%identity, 76.97% similarity) APG01718 31  32 Cry 35, 40, 45, 50, 55, 60,55, 60, 65, 70, 75, 80, WP_057507959.1 (34.97% identity, 51.67% 65, 70,75, 80, 85, 90, 85, 90, 95, 96, 97, 98, similarity) 95, 96, 97, 98, 9999 WP_042612996.1 (34.36% identity, 47.41% similarity) WP_042612995.1(34.02% identity, 52.26% similarity) Cry1If1 (17.86% identity, 28.05%similarity) APG01742 33  34 Cry 90, 95, 96, 97, 98, 99 95, 96, 97, 98,99 AGA40064.1 (86.50% identity, 91.97% similarity) APG00672 (85.22%identity, 91.39% similarity) APG00045 - US_2016_0304898_A1-73 (83.67%identity, 89.34% similarity) US_8318900_B2-69 (78.70% identity, 83.93%similarity) APG01644 (61.25% identity, 74.02% similarity) APG00110 -US_2016_0177333_A1-60 (60.97% identity, 72.96% similarity)US_8318900_B2-207 (58.00% identity, 63.05% similarity) Cry70Bb1 (23.80%identity, 40.54% similarity) APG01772 35 Cry 100 100 WP_017762616.1(99.64% identity, 99.82% similarity) WP_048536362.1 (33.86% identity,47.63% similarity) APG00603 - US_2016_0311864_A1-198 (32.86% identity,49.65% similarity) APG00285.1 - US_2016_0311864_A1-303 (32.51% identity,48.02% similarity) APG01780 36  37 Cry32 75, 80, 85, 90, 95, 96, 85, 90,95, 96, 97, 98, APG07803 (72.78% identity, 80.76% similarity) 97, 98, 9999 AGP18022.1 (71.22% identity, 82.31% similarity) APG00007 -US_2016_0304898_A1-11 (70.83% identity, 82.23% similarity) APG00188 -US_2016_0311864_A1-57 (69.15% identity, 78.92% similarity) APG06253(69.06% identity, 78.99% similarity) APG01915 (64.66% identity, 75.27%similarity) US_2013_0227743_A1-48 (44.55% identity, 56.40% similarity)AEH76820.1 (44.47% identity, 58.41% similarity) Cry32Ca1 (43.34%identity, 57.60% similarity) APG01790 38  39 Bin 95, 96, 97, 98, 99 95,96, 97, 98, 99 APG00118 - US_2016_0311864_A1-28 (91.01% identity, 94.01%similarity) J8MIQ2_BACCE (90.16% identity, 91.10% similarity) APG00454 -US_2016_0311864_A1-179 (90.09% identity, 94.70% similarity) APG00335(89.17% identity, 94.47% similarity) APG00242 - US_2016_0311864_A1-93(88.94% identity, 94.24% similarity) APG00913 - US_2016_0311864_A1-226(84.48% identity, 88.91% similarity) APG04176 (51.47% identity, 66.44%similarity) APG05715 (51.47% identity, 65.76% similarity) APG02445(51.25% identity, 65.83% similarity) Cry35Ab4 (19.38% identity, 36.56%similarity) APG01849 40 41, 42 MTX 85, 90, 95, 96, 97, 98, 90, 95, 96,97, 98, 99 WP_051965558.1 (83.82% identity, 88.89% 99 similarity)WP_051742725.1 (32.75% identity, 45.49% similarity) SDS90165.1 (32.31%identity, 43.96% similarity) WP_067035588.1 (26.22% identity, 40.59%similarity) APG01915 43  44 Cry32 70, 75, 80, 85, 90, 95, 85, 90, 95,96, 97, 98, APG06253 (74.69% identity, 83.54% similarity) 96, 97, 98, 9999 APG00188 - US_2016_0311864_A1-57 (73.85% identity, 82.77% similarity)APG07803 (70.57% identity, 80.58% similarity) AGP18022.1 (69.43%identity, 80.31% similarity) APG00007 - US_2016_0304898_A1-11 (68.97%identity, 79.23% similarity) APG01780 (64.66% identity, 75.27%similarity) US_2013_0227743_A1-48 (45.77% identity, 58.76% similarity)Cry32Ca1 (44.61% identity, 58.26% similarity) APG01937 45 Bin 30, 35,40, 45, 50, 55, 40, 45, 50, 55, 60, 65, WP_063780311.1 (27.05% identity,37.68% 60, 65, 70, 75, 80, 85, 70, 75, 80, 85, 90, 95, similarity) 90,95, 96, 97, 98, 99 96, 97, 98, 99 SDT78826.1 (22.01% identity, 26.18%similarity) KOU65337.1 (21.79% identity, 27.93% similarity) Cry34Ac2(21.10% identity, 27.98% similarity) APG01996 46 MTX 25, 30, 35, 40, 45,50, 45, 50, 55, 60, 65, 70, APG02633 (33.80% identity, 52.61%similarity) 55, 60, 65, 70, 75, 80, 75, 80, 85, 90, 95, 96, APG06768(29.18% identity, 44.59% similarity) 85, 90, 95, 96, 97, 98, 97, 98, 99APG00236 (28.57% identity, 50.32% similarity) 99 APG06880 (28.26%identity, 44.72% similarity) APG00598 (27.48% identity, 44.09%similarity) APG07983 (26.40% identity, 41.25% similarity) APG09455(26.07% identity, 42.94% similarity) US_2015_0047076_A1-11 (24.28%identity, 42.49% similarity) APG02168 47 MTX 35, 40, 45, 50, 55, 60, 50,55, 60, 65, 70, 75, APG09232 (76.88% identity, 85.84% similarity) 65,70, 75, 80, 85, 90, 80, 85, 90, 95, 96, 97, WP_065845806.1 (33.9%identity, 49.15% similarity) 95, 96, 97, 98, 99 98, 99 SDW46809.1(29.27% identity, 48.51% similarity) SDX17518.1 (27.81% identity, 44.65%similarity) WP_006918908.1 (22.44% identity, 41.0% similarity) APG0221548  49 Bin 50, 55, 60, 65, 70, 75, 65, 70, 75, 80, 85, 90,WP_010169077.1 (49.89% identity, 62.58% 80, 85, 90, 95, 96, 97, 95, 96,97, 98, 99 similarity) 98, 99 SFP27084.1 (47.76% identity, 61.41%similarity) SET43751.1 (47.55% identity, 61.41% similarity) SFB00510.1(47.55% identity, 61.41% similarity) APG02262 50 Cry 20, 25, 30, 35, 40,45, 20, 25, 30, 35, 40, 45, WP_025143599.1 (16.96% identity, 17.62% 50,55, 60, 65, 70, 75, 50, 55, 60, 65, 70, 75, similarity) 80, 85, 90, 95,96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98, 99 APG02291 51 Cry34 95, 96,97, 98, 99 96, 97, 98, 99 WP_069325540.1 (92.17% identity, 95.65%similarity) APG01522 (90.6% identity, 94.02% similarity) WP_046215736.1(89.57% identity, 93.04% similarity) WP_052520572.1 (62.61% identity,77.39% similarity) Cry34Ac3 (47.58% identity, 57.26% similarity)APG02293 52  53 MTX 98, 99 100 WP_050845726.1 (97.85% identity, 99.69%similarity) WP_000823322.1 (96.93% identity, 98.77% similarity)WP_016084062.1 (96.63% identity, 98.47% similarity) WP_016085044.1(96.32% identity, 98.16% similarity) APG07036 (94.17% identity, 94.79%similarity) APG04804 (69.02% identity, 80.98% similarity) APG04640(68.4% identity, 81.29% similarity) APG06161 (68.1% identity, 81.6%similarity) APG02408 54 55, 56 MTX 95, 96, 97, 98, 99 95, 96, 97, 98, 99WP_040729904.1 (90.65% identity, 94.39% similarity) EHQ63877.1 (85.98%identity, 89.1% similarity) WP_019421057.1 (77.47% identity, 86.73%similarity) WP_006285537.1 (73.72% identity, 83.38% similarity) APG0244557  58 Bin 90, 95, 96, 97, 98, 99 95, 96, 97, 98, 99 APG05715 (96.73%identity, 98.99% similarity) APG04176 (94.47% identity, 96.98%similarity) WP_070144216.1 (88.94% identity, 93.72% similarity) APG00806(88.44% identity, 94.47% similarity) SDE18889.1 (86.25% identity, 93.25%similarity) APG00212.1 - US_2016_0311864_A1-259 (86.00% identity, 91.15%similarity) APG00212 - US_2016_0311864_A1-75 (84.13% identity, 89.18%similarity) APG07818 (81.33% identity, 88.21% similarity) APG00592 -US_2016_0311864_A1-195 (80.54% identity, 87.35% similarity) APG00619 -US_2016_0311864_A1-199 (80.15% identity, 88.34% similarity) APG00798 -US_2016_0311864_A1-219 (78.90% identity, 86.09% similarity) APG01790(51.25% identity, 65.83% similarity) Cry35Ba1 (23.48% identity, 38.04%similarity) APG02477 59  60 MTX 20, 25, 30, 35, 40, 45, 30, 35, 40, 45,50, 55, APG07002 (50.49% identity, 51.14% similarity) 50, 55, 60, 65,70, 75, 60, 65, 70, 75, 80, 85, WP_025150761.1 (18.10% identity, 26.69%80, 85, 90, 95, 96, 97, 90, 95, 96, 97, 98, 99 similarity) 98, 99WP_016131662.1 (17.48% identity, 27.91% similarity) WP_016113347.1(16.98% identity, 27.47% similarity) WP_002193657.1 (15.32% identity,26.13% similarity) APG02638 61 MTX 80, 85, 90, 95, 96, 97, 90, 95, 96,97, 98, 99 APG00749 (89.70% identity, 95.02% similarity) 98, 99 APG03379(87.63% identity, 93.98% similarity) US_2013_0227743_A1-100 (79.00%identity, 86.00% similarity) APG00006.2 - US_2016_0304898_A1-228 (78.93%identity, 89.97% similarity) APG07655 (78.93% identity, 87.96%similarity) J8YPM2_BACCE (78.33% identity, 85.67% similarity)APG00201.1 - US_2016_0304898_A1-194 (78.26% identity, 88.63% similarity)APG02279 (78.10% identity, 85.95% similarity) APG08088 (77.81% identity,87.42% similarity) APG09682 (77.15% identity, 86.42% similarity)APG00006 - US_2016_0304898_A1-9 (77.12% identity, 87.91% similarity)APG00566 (76.47% identity, 89.22% similarity) APG01086 (75.49% identity,88.24% similarity) APG02782 62 63, 64 MTX 80, 85, 90, 95, 96, 97, 90,95, 96, 97, 98, 99 APG02960 (84.97% identity, 93.56% similarity) 98, 99APG04643 (84.05% identity, 93.56% similarity) APG08085 (82.82% identity,88.96% similarity) APG02555 (80.98% identity, 88.04% similarity)APG00743 (79.75% identity, 87.73% similarity) WP_044444098.1 (79.75%identity, 87.73% similarity) WP_060749709.1 (77.06% identity, 85.93%similarity) SFC26517.1 (75.54% identity, 83.49% similarity)WP_016132978.1 (51.78% identity, 65.68% similarity) APG05651 (51.49%identity, 66.07% similarity) APG05084 (50.59% identity, 64.20%similarity) APG02798 65  66 MTX 55, 60, 65, 70, 75, 80, 80, 85, 90, 95,96, 97, W0LFY6_SERFO (53.52% identity, 75.00% 85, 90, 95, 96, 97, 98,98, 99 similarity) 99 CA_2753918-21 (30.53% identity, 47.04% similarity)R8CLR6_BACCE (30.22% identity, 47.35% similarity) S2KUF5_BACCE (30.22%identity, 47.35% similarity) APG02809 67 MTX 75, 80, 85, 90, 95, 96, 85,90, 95, 96, 97, 98, WP_000982046.1 (71.48% identity, 81.75% 97, 98, 9999 similarity) ADY24997.2 (70.15% identity, 80.22% similarity)AAA22332.1 (25.08% identity, 44.05% similarity) WP_033694890.1 (20.00%identity, 35.07% similarity) APG02912 68  69 MACPF 45, 50, 55, 60, 65,70, 65, 70, 75, 80, 85, 90, APG03726 (72.73% identity, 83.00%similarity) 75, 80, 85, 90, 95, 96, 95, 96, 97, 98, 99 APG04597 (54.65%identity, 62.48% similarity) 97, 98, 99 WP_019961352.1 (44.84% identity,60.52% similarity) WP_046018755.1 (34.98% identity, 51.14% similarity)WP_012039071.1 (34.74% identity, 52.40% similarity) WP_012145116.1(34.45% identity, 56.30% similarity) APG03015 70  71 MTX 75, 80, 85, 90,95, 96, 85, 90, 95, 96, 97, 98, APG00344 (88.22% identity, 93.39%similarity) 97, 98, 99 99 APG00477 (71.26% identity, 83.05% similarity)R8S542_BACCE (70.98% identity, 82.76% similarity) APG00384 (57.26%identity, 72.08% similarity) R8S773_BACCE (45.94% identity, 64.15%similarity) APG00982 - US_2016_0304898_A1-226 (45.61% identity, 63.17%similarity) APG00092 - US_2016_0304898_A1-122 (45.53% identity, 60.89%similarity) APG03053 72  73 MTX 85, 90, 95, 96, 97, 98, 95, 96, 97, 98,99 APG00098.1 - US_2016_0304898_A1-129 (84.92% 99 identity, 91.38%similarity) AGA40063.1 (84.62% identity, 90.15% similarity) APG00098 -US_2016_0304898_A1-128 (83.29% identity, 89.63% similarity) APG00899(83.00% identity, 89.05% similarity) Cry60Ba2 (32.07% identity, 46.65%similarity) APG03072 74  75 MTX 100 100 WP_047426867.1 (99.70% identity,99.70% similarity) WP_062673074.1 (92.12% identity, 96.36% similarity)APG02518 (88.18% identity, 93.33% similarity) APG07574 (87.88% identity,93.33% similarity) APG03185 (87.27% identity, 92.73% similarity)APG03114 (78.98% identity, 90.69% similarity) WP_034734550.1 (78.55%identity, 91.54% similarity) WP_027372020.1 (77.95% identity, 90.94%similarity) APG03080 76 Cyt 97, 98, 99 99 APG00388 -US_2016_0311864_A1-159 (99.58% identity, 100.0% similarity) SAY46370.1(96.25% identity, 98.75% similarity) WP_058393986.1 (43.97% identity,59.92% similarity) Cyt1Aa6 (30.47% identity, 51.56% similarity) APG0322777  78 MTX 60, 65, 70, 75, 80, 85, 75, 80, 85, 90, 95, 96, APG03867(82.45% identity, 89.97% similarity) 90, 95, 96, 97, 98, 99 97, 98, 99APG00143 (66.36% identity, 78.40% similarity) APG00444 (64.69% identity,78.13% similarity) APG00441 (64.69% identity, 77.50% similarity)APG00833 (62.31% identity, 77.88% similarity) WP_065845806.1 (58.51%identity, 71.52% similarity) SDX17518.1 (48.53% identity, 64.41%similarity) SDW46809.1 (46.08% identity, 63.25% similarity)WP_006918908.1 (31.71% identity, 50.00% similarity) APG03249 79  80 MTX35, 40, 45, 50, 55, 60, 50, 55, 60, 65, 70, 75, APG00601 (97.86%identity, 97.86% similarity) 65, 70, 75, 80, 85, 90, 80, 85, 90, 95, 96,97, APG00091 - US_2016_0304898_A1-120 (96.07% 95, 96, 97, 98, 99 98, 99identity, 97.50% similarity) APG00021 - US_2016_0304898_A1-35 (95.36%identity, 97.86% similarity) APG00091.1 - US_2016_0304898_A1-121 (94.29%identity, 95.71% similarity) APG00850 (75.36% identity, 87.5%similarity) Cry45Aa (31.16% identity, 46.58% similarity) APG03299 81 MTX75, 80, 85, 90, 95, 96, 75, 80, 85, 90, 95, 96, APG09256 (73.64%identity, 75.19% similarity) 97, 98, 99 97, 98, 99 WP_000586614.1(72.20% identity, 73.75% similarity) WP_000586616.1 (72.20% identity,73.75% similarity) WP_063549995.1 (71.81% identity, 73.36% similarity)APG09842 (70.27% identity, 73.36% similarity) APG02768 (69.11% identity,72.97% similarity) APG02429 (69.11% identity, 72.59% similarity)APG01103 (68.73% identity, 72.97% similarity) Cry46Ab (22.73% identity,33.77% similarity) APG03309 82  83 Cyt 80, 85, 90, 95, 96, 97, 90, 95,96, 97, 98, 99 APG08780 (88.66% identity, 93.30% similarity) 98, 99WP_030313032.1 (78.87% identity, 88.66% similarity) APG00945 (78.57%identity, 85.20% similarity) APG00134 - US_2016_0311864_A1-32 (78.35%identity, 88.14% similarity) WP_055599179.1 (78.35% identity, 88.14%similarity) APG00327 (72.48% identity, 74.03% similarity) APG00108 -US_2016_0311864_A1-22 (57.46% identity, 64.18% similarity) Cyt2Ca1(25.11% identity, 43.72% similarity) APG03379 84 MTX 80, 85, 90, 95, 96,97, 90, 95, 96, 97, 98, 99 APG00749 (88.33% identity, 95.00% similarity)98, 99 APG02638 (87.63% identity, 93.98% similarity) APG07655 (81.88%identity, 88.93% similarity) APG09682 (81.54% identity, 88.26%similarity) APG00006.2 - US_2016_0304898_A1-228 (80.87% identity, 91.28%similarity) APG00201.1 - US_2016_0304898_A1-194 (80.87% identity, 89.60%similarity) APG08088 (79.4% identity, 88.70% similarity) APG00006 -US_2016_0304898_A1-9 (79.02% identity, 89.18% similarity) APG00201 -US_2016_0304898_A1-193 (79.02% identity, 87.54% similarity) APG00847 -US_2016_0304898_A1-223 (78.36% identity, 87.21% similarity) APG00566(78.10% identity, 87.91% similarity) J8YPM2_BACCE (77.59% identity,85.62% similarity) APG03519 85 86, 87 Cry32 85, 90, 95, 96, 97, 98, 90,95, 96, 97, 98, 99 Cry32Da1 (81.69% identity, 87.31% similarity) 99APG00710 (57.21% identity, 68.35% similarity) APG00056 -US_2016_0311864_A1-1 (56.79% identity, 67.22% similarity) APG01329(55.50% identity, 67.16% similarity) APG00430 (55.30% identity, 66.64%similarity) APG00504 (54.68% identity, 64.86% similarity) APG00068 -US_2016_0177333_A1-35 (53.91% identity, 65.14% similarity) APG06739(53.25% identity, 64.35% similarity) APG01387 (53.17% identity, 65.72%similarity) APG06650 (52.74% identity, 65.03% similarity) APG03569 88Cry 30, 35, 40, 45, 50, 55, 50, 55, 60, 65, 70, 75,US_2013_0227743_A1-184 (28.75% identity, 45.80% 60, 65, 70, 75, 80, 85,80, 85, 90, 95, 96, 97, similarity) 90, 95, 96, 97, 98, 99 98, 99APG00267.2 - US_2016_0311864_A1-294 (24.90% identity, 37.07% similarity)WP_048536362.1 (24.07% identity, 35.42% similarity) WP_048536363.1(23.32% identity, 34.28% similarity) APG03604 89 MTX 96, 97, 98, 99 100SDI38744.1 (95.70% identity, 99.28% similarity) WP_065397681.1 (88.89%identity, 94.62% similarity) I0KFP2_9BACT (39.73% identity, 57.91%similarity) WP_053730552.1 (38.69% identity, 55.65% similarity) APG0369990  91 Cry 35, 40, 45, 50, 55, 60, 45, 50, 55, 60, 65, 70,US_2013_0227743_A1-152 (30.76% identity, 43.53% 65, 70, 75, 80, 85, 90,75, 80, 85, 90, 95, 96, similarity) 95, 96, 97, 98, 99 97, 98, 99WP_048536362.1 (27.72% identity, 42.83% similarity)US_2013_0227743_A1-202 (27.22% identity, 40.90% similarity) Cry1Id1(13.45% identity, 24.97% similarity) APG03722 92 93, 94 Cry32 80, 85,90, 95, 96, 97, 85, 90, 95, 96, 97, 98, AGA40028.1 (75.75% identity,81.78% similarity) 98, 99 99 AGP18036.1 (59.38% identity, 71.31%similarity) AGP18046.1 (53.44% identity, 66.39% similarity) Cry4Ba1(28.29% identity, 41.69% similarity) APG03726 95  96 MACPF 50, 55, 60,65, 70, 75, 65, 70, 75, 80, 85, 90, APG04597 (73.33% identity, 73.63%similarity) 80, 85, 90, 95, 96, 97, 95, 96, 97, 98, 99 APG02912 (72.73%identity, 83.00% similarity) 98, 99 WP_019961352.1 (48.54% identity,63.35% similarity) WP_012039071.1 (37.38% identity, 53.95% similarity)WP_046018755.1 (36.94% identity, 53.58% similarity) WP_012145116.1(33.72% identity, 55.56% similarity) APG03732 97  98 MTX 40, 45, 50, 55,60, 65, 55, 60, 65, 70, 75, 80, APG01517 (95.22% identity, 97.91%similarity) 70, 75, 80, 85, 90, 95, 85, 90, 95, 96, 97, 98,US_2013_0227743_A1-114 (35.81% identity, 53.99% 96, 97, 98, 99 99similarity) US_2013_0227743_A1-112 (34.44% identity, 54.27% similarity)ADK08315.1 (25.70% identity, 44.69% similarity) AIK29697.1 (23.83%identity, 41.45% similarity) APG03746 99 100, 101 MTX 95, 96, 97, 98, 9998, 99 APG00049 - US_2016_0304898_A1-78 (96.27% identity, 98.31%similarity) WP_000730625.1 (94.92% identity, 97.29% similarity)WP_003290257.1 (91.86% identity, 94.14% similarity) APG00049.1 -US_2016_0304898_A1-79 (86.44% identity, 88.47% similarity) APG00013 -US_2016_0304898_A1-22 (50.16% identity, 67.19% similarity) APG03786 102103 Cry59 70, 75, 80, 85, 90, 95, 80, 85, 90, 95, 96, 97, AEX56526.1(67.74% identity, 78.54% similarity) 96, 97, 98, 99 98, 99 Cry59Aa1(61.23% identity, 70.96% similarity) APG03848 104 105 MTX 35, 40, 45,50, 55, 60, 50, 55, 60, 65, 70, 75, WP_065486138.1 (33.24% identity,49.47% 65, 70, 75, 80, 85, 90, 80, 85, 90, 95, 96, 97, similarity) 95,96, 97, 98, 99 98, 99 WP_065487080.1 (33.24% identity, 49.47%similarity) J8Y0J8_BACCE (32.17% identity, 48.53% similarity)US_2008_0070829_A1-11 (31.77% identity, 47.40% similarity) APG03901 106107, 108 Cry 30, 35, 40, 45, 50, 55, 50, 55, 60, 65, 70, 75,US_2013_0227743_A1-198 (27.16% identity, 45.00% 60, 65, 70, 75, 80, 85,80, 85, 90, 95, 96, 97, similarity) 90, 95, 96, 97, 98, 99 98, 99WP_048536348.1 (24.07% identity, 39.24% similarity) APG00061 -US_2016_0304898_A1-92 (23.88% identity, 41.32% similarity) Cry70Bb1(20.6% identity, 35.9% similarity) APG03947 109 110, 111 MTX 100 100B1HN08_LYSSC (99.72% identity, 100.00% similarity) WP_051563179.1(96.62% identity, 96.62% similarity) WP_051800214.1 (96.34% identity,96.62% similarity) WP_051891081.1 (96.34% identity, 96.62% similarity)APG04013 112 113 MTX 35, 40, 45, 50, 55, 60, 45, 50, 55, 60, 65, 70,APG00014 - US_2016_0304898_A1-24 (84.62% 65, 70, 75, 80, 85, 90, 75, 80,85, 90, 95, 96, identity, 87.76% similarity) 95, 96, 97, 98, 99 97, 98,99 WP_033694890.1 (31.65% identity, 42.55% similarity) AF316145_1 (29.9%identity, 47.84% similarity) AAA22332.1 (28.06% identity, 41.79%similarity) APG04076 114 115 MTX 75, 80, 85, 90, 95, 96, 85, 90, 95, 96,97, 98, APG00201 - US_2016_0304898_A1-193 (76.07% 97, 98, 99 99identity, 86.56% similarity) APG00847 - US_2016_0304898_A1-223 (75.74%identity, 86.56% similarity) APG00036 - US_2016_0304898_A1-60 (74.92%identity, 85.15% similarity) APG00201.1 - US_2016_0304898_A1-194 (74.75%identity, 84.92% similarity) APG09682 (74.43% identity, 84.92%similarity) APG00260 (74.10% identity, 85.57% similarity) APG04720(72.94% identity, 83.83% similarity) APG00006 - US_2016_0304898_A1-9(72.55% identity, 84.97% similarity) APG06690 (72.37% identity, 84.21%similarity) APG08088 (71.95% identity, 84.82% similarity)US_2013_0227743_A1-100 (70.59% identity, 82.03% similarity) APG04176 116117 Bin 90, 95, 96, 97, 98, 99 95, 96, 97, 98, 99 APG02445 (94.47%identity, 96.98% similarity) APG05715 (93.72% identity, 96.98%similarity) WP_070144216.1 (89.45% identity, 94.47% similarity) APG00806(88.44% identity, 94.47% similarity) APG00212.1 - US_2016_0311864_A1-259(87.84% identity, 92.56% similarity) SDE18889.1 (86.75% identity, 93.25%similarity) APG00212 - US_2016_0311864_A1-75 (85.92% identity, 90.53%similarity) APG07818 (82.38% identity, 89.33% similarity) APG00619 -US_2016_0311864_A1-199 (80.69% identity, 89.11% similarity) APG00592 -US_2016_0311864_A1-195 (80.58% identity, 87.62% similarity) APG00798 -US_2016_0311864_A1-219 (80.15% identity, 87.41% similarity) APG01790(51.47% identity, 66.44% similarity) Cry35Ad1 (20.09% identity, 36.30%similarity) APG04332 118 119 Cry8 45, 50, 55, 60, 65, 70, 60, 65, 70,75, 80, 85, WP_061885066.1 (44.33% identity, 57.76% 75, 80, 85, 90, 95,96, 90, 95, 96, 97, 98, 99 similarity) 97, 98, 99 US_2013_0227743_A1-70(35.29% identity, 45.42% similarity) AGP18068.1 (31.99% identity, 41.35%similarity) Cry8Aa1 (30.55% identity, 39.39% similarity) APG04350 120121 Cry32 60, 65, 70, 75, 80, 85, 70, 75, 80, 85, 90, 95, AGP18058.1(57.10% identity, 68.41% similarity) 90, 95, 96, 97, 98, 99 96, 97, 98,99 AGP18029.1 (54.09% identity, 66.03% similarity) AGP18037.1 (53.98%identity, 67.38% similarity) Cry32Ea1 (43.50% identity, 56.78%similarity) APG04365 122 123 Vip4 45, 50, 55, 60, 65, 70, 60, 65, 70,75, 80, 85, WP_000769710.1 (43.36% identity, 59.54% 75, 80, 85, 90, 95,96, 90, 95, 96, 97, 98, 99 similarity) 97, 98, 99 SCC34829.1 (43.22%identity, 57.37% similarity) Vip4Aa1 (42.41% identity, 56.91%similarity) APG04418 124 125, 126 Cry22 50, 55, 60, 65, 70, 75, 65, 70,75, 80, 85, 90, APG09096 (57.34% identity, 70.45% similarity) 80, 85,90, 95, 96, 97, 95, 96, 97, 98, 99 APG00490 - US_2016_0177333_A1-143(47.28% 98, 99 identity, 61.35% similarity) APG00490.1 -US_2016_0177333_A1-144 (47.28% identity, 61.35% similarity)WP_017154368.1 (46.72% identity, 61.54% similarity) Cry22Bb1 (37.55%identity, 47.74% similarity) APG04431 127 128 MTX 90, 95, 96, 97, 98, 9995, 96, 97, 98, 99 US_2013_0227743_A1-120 (87.02% identity, 91.35%similarity) APG00202 - US_2016_0304898_A1-195 (85.61% identity, 91.92%similarity) APG00208 - US_2016_0304898_A1-201 (81.66% identity, 89.95%similarity) APG00352 (75.19% identity, 85.32% similarity) J8F337_BACCE(74.11% identity, 82.49% similarity) US_2013_0227743_A1-122 (32.52%identity, 48.66% similarity) APG04460 129 130 131 Cry 55, 60, 65, 70,75, 80, 65, 70, 75, 80, 85, 90, WP_050845419.1 (54.74% identity, 62.47%85, 90, 95, 96, 97, 98, 95, 96, 97, 98, 99 similarity) 99 AFM73704.1(50.07% identity, 57.32% similarity) AGP18009.1 (39.08% identity, 49.57%similarity) Cry53Aa1 (33.75% identity, 46.50% similarity) APG04477 132133 MTX 60, 65, 70, 75, 80, 85, 75, 80, 85, 90, 95, 96, APG00569 (91.67%identity, 94.05% similarity) 90, 95, 96, 97, 98, 99 97, 98, 99 APG00938(89.88% identity, 94.05% similarity) APG00563 (84.23% identity, 89.29%similarity) APG09055 (81.85% identity, 88.39% similarity) APG00794(78.64% identity, 86.65% similarity) APG00590 - US_2016_0311864_A1-193(58.60% identity, 74.64% similarity) C3GC23_BACTU (57.14% identity,70.54% similarity) APG00146 - US_2016_0304898_A1-166 (57.14% identity,70.24% similarity) ANN35739.1 (55.92% identity, 71.60% similarity)APG04597 134 135 MACPF 40, 45, 50, 55, 60, 65, 50, 55, 60, 65, 70, 75,APG03726 (73.33% identity, 73.63% similarity) 70, 75, 80, 85, 90, 95,80, 85, 90, 95, 96, 97, APG02912 (54.65% identity, 62.48% similarity)96, 97, 98, 99 98, 99 WP_019961352.1 (36.26% identity, 47.22%similarity) WP_012039071.1 (28.20% identity, 41.13% similarity)WP_046018755.1 (26.20% identity, 37.89% similarity) WP_012145116.1(25.29% identity, 41.37% similarity) APG04598 136 MTX 30, 35, 40, 45,50, 55, 50, 55, 60, 65, 70, 75, APG04483 (80.66% identity, 92.79%similarity) 60, 65, 70, 75, 80, 85, 80, 85, 90, 95, 96, 97, APG06465(80.33% identity, 92.46% similarity) 90, 95, 96, 97, 98, 99 98, 99APG00696 (80.00% identity, 92.13% similarity) APG01882 (79.41% identity,91.83% similarity) APG03715 (76.56% identity, 87.19% similarity)APG00016.1 - US_2016_0304898_A1-27 (47.39% identity, 62.42% similarity)APG00016 - US_2016_0304898_A1-26 (45.03% identity, 59.32% similarity)AGP18028.1 (26.75% identity, 45.54% similarity) Cry23Aa1 (25.77%identity, 42.64% similarity) APG04640 137 138 MTX 90, 95, 96, 97, 98, 9995, 96, 97, 98, 99 APG06161 (92.64% identity, 96.01% similarity)WP_016099386.1 (85.58% identity, 90.18% similarity) APG04804 (70.55%identity, 84.05% similarity) WP_016084062.1 (69.33% identity, 82.21%similarity) WP_016085044.1 (69.33% identity, 82.21% similarity)WP_016084449.1 (69.02% identity, 82.21% similarity) APG02293 (68.40%identity, 81.29% similarity) APG07036 (66.56% identity, 78.83%similarity) APG04682 139 140 Cry 35, 40, 45, 50, 55, 60, 50, 55, 60, 65,70, 75, US_2006_0212965_A1-4 (33.12% identity, 48.12% 65, 70, 75, 80,85, 90, 80, 85, 90, 95, 96, 97, similarity) 95, 96, 97, 98, 99 98, 99US_2011_0203014-17 (32.15% identity, 48.40% similarity) CA_2790023-17(32.15% identity, 48.40% similarity) Cry21Ga1 (29.61% identity, 46.61%similarity) APG04720 141 142 MTX 75, 80, 85, 90, 95, 96, 85, 90, 95, 96,97, 98, APG00036 - US_2016_0304898_A1-60 (94.98% 97, 98, 99 99 identity,95.65% similarity) APG00036.1 - US_2016_0304898_A1-61 (94.65% identity,95.65% similarity) APG00036.2 - US_2016_0304898_A1-229 (93.98% identity,94.65% similarity) APG08088 (79.93% identity, 86.96% similarity)APG00201 - US_2016_0304898_A1-193 (76.72% identity, 83.93% similarity)APG01506 (76.16% identity, 84.44% similarity) APG00566 (75.24% identity,84.04% similarity) APG02279 (75.16% identity, 83.66% similarity)APG00847 - US_2016_0304898_A1-223 (75.08% identity, 82.95% similarity)J8YPM2_BACCE (73.75% identity, 83.72% similarity) APG04076 (72.94%identity, 83.83% similarity) APG06690 (72.79% identity, 82.62%similarity) APG04725 143 144 Cyt 85, 90, 95, 96, 97, 98, 90, 95, 96, 97,98, 99 APG00126.1 - US_2016_0304898_A1-154 (82.91% 99 identity, 93.16%similarity) APG00126 - US_2016_0304898_A1-153 (80.83% identity, 90.83%similarity) WP_016110460.1 (80.08% identity, 89.63% similarity) APG00437(78.00% identity, 86.00% similarity) APG08631 (75.60% identity, 85.20%similarity) APG07961 (60.58% identity, 78.42% similarity) APG08230(57.14% identity, 75.79% similarity) APG00128 - US_2016_0304898_A1-157(56.85% identity, 73.86% similarity) APG00177 - US_2016_0311864_A1-52(56.75% identity, 75.00% similarity) APG00121 - US_2016_0311864_A1-29(52.92% identity, 67.88% similarity) Cyt2Ca1 (40.87% identity, 56.75%similarity) APG04804 145 146 MTX 70, 75, 80, 85, 90, 95, 85, 90, 95, 96,97, 98, 99 APG06161 (70.86% identity, 84.36% similarity) 96, 97, 98, 99APG04640 (70.55% identity, 84.05% similarity) WP_016084062.1 (69.63%identity, 81.60% similarity) WP_016085044.1 (69.33% identity, 81.29%similarity) APG02293 (69.02% identity, 80.98% similarity) WP_016084449.1(69.02% identity, 80.98% similarity) WP_000823322.1 (68.40% identity,80.98% similarity) APG07036 (66.87% identity, 77.30% similarity)APG04807 147 148 MTX 25, 30, 35, 40, 45, 50, 45, 50, 55, 60, 65, 70,APG08307 (51.20% identity, 65.87% similarity) 55, 60, 65, 70, 75, 80,75, 80, 85, 90, 95, 96, US_2013_0227743_A1-114 (23.26% identity, 40.31%85, 90, 95, 96, 97, 98, 97, 98, 99 similarity) 99 AGP17988.1 (14.71%identity, 24.22% similarity) APG04931 149 150 Vip4 65, 70, 75, 80, 85,90, 80, 85, 90, 95, 96, 97, WP_000769710.1 (63.94% identity, 72.61% 95,96, 97, 98, 99 98, 99 similarity) WP_048561796.1 (63.31% identity,75.17% similarity) SCL96146.1 (63.31% identity, 74.88% similarity)APG09545 (59.26% identity, 70.37% similarity) Vip4Aa1 (58.28% identity,73.05% similarity) APG09984 (55.58% identity, 69.35% similarity)APG00106 - US_2016_0304898_A1-134 (55.13% identity, 69.14% similarity)APG07790 (51.38% identity, 65.84% similarity) APG04978 151 MTX 25, 30,35, 40, 45, 50, 45, 50, 55, 60, 65, 70, APG08043 (31.11% identity,45.71% similarity) 55, 60, 65, 70, 75, 80, 75, 80, 85, 90, 95, 96,APG07738 (30.89% identity, 43.12% similarity) 85, 90, 95, 96, 97, 98,97, 98, 99 APG02633 (24.41% identity, 42.37% similarity) 99 APG06768(23.89% identity, 40.76% similarity) Cry33Aa1 (23.80% identity, 36.14%similarity) Cry51Aa1 (23.80% identity, 35.84% similarity) Cry64Aa1(23.44% identity, 40.63% similarity) APG05025 152 153 Vip3 25, 30, 35,40, 45, 50, 40, 45, 50, 55, 60, 65, APG00071 - US_2016_0311864_A1-8(88.49% 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 95, identity, 93.53%similarity) 85, 90, 95, 96, 97, 98, 96, 97, 98, 99 APG00150 -US_2016_0311864_A1-35 (88.31% 99 identity, 93.53% similarity)APG00071.1 - US_2016_0311864_A1-9 (87.95% identity, 92.99% similarity)Vip3Aa22 (22.09% identity, 35.17% similarity) APG05034 154 155, 156 Cry40, 45, 50, 55, 60, 65, 60, 65, 70, 75, 80, 85, APG00061 -US_2016_0304898_A1-92 (44.40% 70, 75, 80, 85, 90, 95, 90, 95, 96, 97,98, 99 identity, 59.43% similarity) 96, 97, 98, 99 WP_048536348.1(39.82% identity, 55.34% similarity) APG00206 - US_2016_0304898_A1-199(37.68% identity, 53.09% similarity) Cry70Ba1 (21.94% identity, 34.09%similarity) APG05045 157 158 MTX 40, 45, 50, 55, 60, 65, 60, 65, 70, 75,80, 85, WP_048178060.1 (35.81% identity, 55.07% 70, 75, 80, 85, 90, 95,90, 95, 96, 97, 98, 99 similarity) 96, 97, 98, 99 WP_004999216.1 (34.85%identity, 55.70% similarity) WP_011306610.1 (34.85% identity, 54.72%similarity) WP_048041904.1 (34.43% identity, 53.11% similarity) APG05084159 160 MTX 100 100 WP_025150761.1 (100.00% identity, 100.00%similarity) APG02921 (89.74% identity, 93.91% similarity) SFS68380.1(89.17% identity, 93.95% similarity) APG06989 (88.14% identity, 93.27%similarity) APG08990 (88.14% identity, 93.27% similarity) APG04226(86.22% identity, 92.31% similarity) APG05969 (86.22% identity, 91.99%similarity) WP_016132978.1 (81.09% identity, 88.78% similarity) APG05651(81.09% identity, 88.14% similarity) APG02782 (50.59% identity, 64.20%similarity) Cry55Aa1 (17.49% identity, 33.00% similarity) APG05328 161MTX 60, 65, 70, 75, 80, 85, 70, 75, 80, 85, 90, 95, APG00559.2 -US_2016_0311864_A1-361 (65.71% 90, 95, 96, 97, 98, 99 96, 97, 98, 99identity, 71.18% similarity) APG00067 - US_2016_0311864_A1-7 (63.82%identity, 70.29% similarity) APG00559 - US_2016_0311864_A1-191 (61.67%identity, 66.86% similarity) APG00559.1 - US_2016_0311864_A1-360 (61.38%identity, 66.86% similarity) APG00954 (59.77% identity, 66.29%similarity) APG00408 - US_2016_0311864_A1-171 (59.60% identity, 66.48%similarity) APG08088 (56.30% identity, 66.86% similarity) APG08589(56.21% identity, 69.23% similarity) APG00260 (55.49% identity, 64.45%similarity) US_2013_0227743_A1-100 (55.03% identity, 65.68% similarity)APG00006 - US_2016_0304898_A1-9 (54.99% identity, 64.96% similarity)APG09682 (54.34% identity, 65.90% similarity) APG05370 162 163 Cry32 85,90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99 APG00054 -US_2016_0304898_A1-84 (84.33% 99 identity, 90.10% similarity)US_2016_0017363_A1-32 (83.63% identity, 89.79% similarity)US_2016_0017363_A1-33 (82.85% identity, 88.93% similarity) APG00310(76.34% identity, 83.10% similarity) APG00974 (72.73% identity, 81.74%similarity) APG00068 - US_2016_0177333_A1-35 (70.36% identity, 80.65%similarity) Cry32Ea1 (62.67% identity, 72.61% similarity) APG06739(61.77% identity, 71.90% similarity) APG05930 (59.88% identity, 69.84%similarity) APG00673 (59.69% identity, 69.34% similarity) APG07100(53.36% identity, 66.57% similarity) APG01329 (53.33% identity, 64.47%similarity) APG05384 164 165, 166 MTX 40, 45, 50, 55, 60, 65, 55, 60,65, 70, 75, 80, APG06912 (78.19% identity, 84.70% similarity) 70, 75,80, 85, 90, 95, 85, 90, 95, 96, 97, 98, US_2013_0227743_A1-112 (36.24%identity, 54.50% 96, 97, 98, 99 99 similarity) US_2013_0227743_A1-114(35.42% identity, 52.34% similarity) AIK29697.1 (24.56% identity, 41.01%similarity) ADK08315.1 (24.35% identity, 40.05% similarity) APG05506 167MTX 80, 85, 90, 95, 96, 97, 90, 95, 96, 97, 98, 99 AGA40030.1 (79.49%identity, 88.20% similarity) 98, 99 US_8829279_B2-37 (79.28% identity,86.46% similarity) APG03760 (64.25% identity, 77.09% similarity)APG05634 (62.29% identity, 75.98% similarity) APG00224 (59.83% identity,72.85% similarity) APG00846 (59.67% identity, 74.31% similarity)APG00609 (58.77% identity, 72.14% similarity) US_8829279_B2-27 (51.10%identity, 66.30% similarity) Q45728_BACTU (44.29% identity, 58.97%similarity) APG05615 168 169 MTX 100 100 J8Y0J8_BACCE (99.70% identity,99.70% similarity) SCA97082.1 (98.80% identity, 99.40% similarity)C3ICE4_BACTU (98.49% identity, 99.40% similarity) J7XTF7_BACCE (92.17%identity, 92.77% similarity) APG05619 170 Bin 35, 40, 45, 50, 55, 60,50, 55, 60, 65, 70, 75, APG00192 - US_2016_0311864_A1-64 (71.83% 65, 70,75, 80, 85, 90, 80, 85, 90, 95, 96, 97, identity, 83.20% similarity) 95,96, 97, 98, 99 98, 99 APG00183 - US_2016_0177333_A1-84 (40.25% identity,58.48% similarity) C2Q549_BACCE (32.49% identity, 47.1% similarity)Cry35Ab2 (23.98% identity, 39.37% similarity) APG05651 171 172 MTX 100100 WP_016131662.1 (100.00% identity, 100.00% similarity) WP_016113347.1(96.45% identity, 98.39% similarity) WP_016132978.1 (96.13% identity,98.71% similarity) APG05969 (83.01% identity, 91.67% similarity)APG02921 (82.69% identity, 89.42% similarity) APG04226 (82.37% identity,90.71% similarity) APG08990 (82.37% identity, 89.74% similarity)APG06989 (81.73% identity, 89.42% similarity) APG05084 (81.09% identity,88.14% similarity) APG02782 (51.49% identity, 66.07% similarity)Cry55Aa1 (19.55% identity, 34.59% similarity) APG05653 173 174 MTX 45,50, 55, 60, 65, 70, 60, 65, 70, 75, 80, 85, WP_065486124.1 (40.51%identity, 56.94% 75, 80, 85, 90, 95, 96, 90, 95, 96, 97, 98, 99similarity) 97, 98, 99 ANS51604.1 (40.06% identity, 56.53% similarity)WP_001267112.1 (39.37% identity, 54.02% similarity) Cry55Aa1 (20.98%identity, 36.1% similarity) APG05658 175 176 MTX 35, 40, 45, 50, 55, 60,50, 55, 60, 65, 70, 75, AGP17985.1 (31.72% identity, 47.46% similarity)65, 70, 75, 80, 85, 90, 80, 85, 90, 95, 96, 97, WP_063226258.1 (31.70%identity, 49.39% 95, 96, 97, 98, 99 98, 99 similarity)US_2016_0311864_A1-267 (31.34% identity, 46.65% similarity) SFT24477.1(30.64% identity, 47.55% similarity) APG05715 177 178 Bin 90, 95, 96,97, 98, 99 95, 96, 97, 98, 99 APG02445 (96.73% identity, 98.99%similarity) APG04176 (93.72% identity, 96.98% similarity) WP_070144216.1(88.44% identity, 93.22% similarity) APG00806 (88.19% identity, 93.97%similarity) APG00212.1 - US_2016_0311864_A1-259 (87.31% identity, 92.54%similarity) SDE18889.1 (86.25% identity, 93.75% similarity) APG00212 -US_2016_0311864_A1-75 (85.40% identity, 90.51% similarity) APG07818(82.34% identity, 89.55% similarity) APG00619 - US_2016_0311864_A1-199(82.13% identity, 89.08% similarity) APG00592 - US_2016_0311864_A1-195(82.00% identity, 88.08% similarity) APG00798 - US_2016_0311864_A1-219(80.10% identity, 87.38% similarity) APG01790 (51.47% identity, 65.76%similarity) Cry49Aa1 (20.08% identity, 33.53% similarity) APG05804 179MTX (Cry64) 85, 90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99 Cry64Aa1(84.92% identity, 89.18% similarity) 99 APG00450 (82.95% identity,86.23% similarity) APG00501 (73.44% identity, 75.08% similarity)APG05810 180 181 Cry 40, 45, 50, 55, 60, 65, 55, 60, 65, 70, 75, 80,APG00558 (71.50% identity, 81.09% similarity) 70, 75, 80, 85, 90, 95,85, 90, 95, 96, 97, 98, V9P857_BACTU (37.83% identity, 50.12% 96, 97,98, 99 99 similarity) Cry24Ba1 (33.96% identity, 48.44% similarity)APG05924 182 183 Vip4 45, 50, 55, 60, 65, 70, 60, 65, 70, 75, 80, 85,WP_000769710.1 (42.54% identity, 57.16% 75, 80, 85, 90, 95, 96, 90, 95,96, 97, 98, 99 similarity) 97, 98, 99 SCL96146.1 (40.53% identity,56.53% similarity) SCC38903.1 (40.45% identity, 55.73% similarity)Vip4Aa1 (40.32% identity, 56.57% similarity) APG05930 184 185 Cry32 70,75, 80, 85, 90, 95, 80, 85, 90, 95, 96, 97, APG00673 (89.88% identity,91.97% similarity) 96, 97, 98, 99 98, 99 Cry32Ea1 (66.12% identity,75.68% similarity) APG00974 (61.29% identity, 71.10% similarity)APG05370 (59.88% identity, 69.84% similarity) APG00054 -US_2016_0304898_A1-84 (59.38% identity, 70.35% similarity) APG06739(58.86% identity, 69.35% similarity) APG00310 (58.80% identity, 70.22%similarity) APG00068 - US_2016_0177333_A1-35 (57.82% identity, 69.46%similarity) APG07100 (53.27% identity, 65.33% similarity) APG03519(50.81% identity, 61.95% similarity) APG05978 186 187 Cry 95, 96, 97,98, 99 96, 97, 98, 99 APG09936 (93.61% identity, 94.52% similarity)WP_049738340.1 (93.38% identity, 95.21% similarity) SEE67477.1 (37.28%identity, 58.26% similarity) WP_036155538.1 (30.74% identity, 50.11%similarity) EXX63903.1 (28.82% identity, 48.39% similarity) APG06051 188Bin 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99 R8CM29_BACCE (86.91%identity, 86.91% similarity) APG00215 - US_2016_0311864_A1-78 (63.23%identity, 71.59% similarity) APG00880 (59.28% identity, 68.98%similarity) APG00386 - US_2016_0311864_A1-158 (55.96% identity, 66.48%similarity) Cry35Ab4 (22.63% identity, 35.79% similarity) APG06161 189190 MTX 90, 95, 96, 97, 98, 99 95, 96, 97, 98, 99 APG04640 (92.64%identity, 96.01% similarity) WP_016099386.1 (86.81% identity, 91.72%similarity) APG04804 (70.86% identity, 84.36% similarity) WP_016084062.1(68.71% identity, 82.52% similarity) WP_016085044.1 (68.71% identity,82.52% similarity) WP_016084449.1 (68.40% identity, 82.21% similarity)APG02293 (68.10% identity, 81.60% similarity) APG07036 (66.26% identity,78.83% similarity) APG06176 191 192 MTX 30, 35, 40, 45, 50, 55, 45, 50,55, 60, 65, 70, Q5PXQ3_BACUD (26.06% identity, 44.24% 60, 65, 70, 75,80, 85, 75, 80, 85, 90, 95, 96, similarity) 90, 95, 96, 97, 98, 99 97,98, 99 APG06253 193 194 Cry32 45, 50, 55, 60, 65, 70, 60, 65, 70, 75,80, 85, APG00188 - US_2016_0311864_A1-57 (95.03% 75, 80, 85, 90, 95, 96,90, 95, 96, 97, 98, 99 identity, 96.51% similarity) 97, 98, 99 APG07803(88.97% identity, 93.37% similarity) APG00007 - US_2016_0304898_A1-11(78.08% identity, 85.54% similarity) APG00007.1 - US_2016_0304898_A1-12(77.54% identity, 84.77% similarity) APG01915 (74.69% identity, 83.54%similarity) APG01780 (69.06% identity, 78.99% similarity) Cry32Ca1(44.29% identity, 57.00% similarity) APG06364 195 196 MTX 65, 70, 75,80, 85, 90, 70, 75, 80, 85, 90, 95, US_2013_0227743_A1_124 (63.95%identity, 66.57% 95, 96, 97, 98, 99 96, 97, 98, 99 similarity)R8R7L4_BACCE (59.33% identity, 67.28% similarity) SDW71198.1 (39.34%identity, 51.80% similarity) Cry55Aa1 (24.34% identity, 39.42%similarity) APG06372 197 198, 199 MTX 98, 99 100 WP_061657790.1 (97.52%identity, 99.01% similarity) W8YCZ9_BACTU (97.27% identity, 98.76%similarity) APG00129 - US_2016_0304898_A1-158 (96.53% identity, 98.76%similarity) APG00129.1 - US_2016_0304898_A1-159 (96.28% identity, 98.76%similarity) APG07748 (82.61% identity, 86.73% similarity) APG00681(62.56% identity, 74.43% similarity) APG00304 (59.01% identity, 71.36%similarity) APG00366 (58.87% identity, 73.89% similarity) APG03484(57.79% identity, 69.53% similarity) APG06428 200 201 Cry30 80, 85, 90,95, 96, 97, 90, 95, 96, 97, 98, 99 WP_050845409.1 (78.87% identity,85.76% 98, 99 similarity) Cry30Ga2 (73.55% identity, 79.94% similarity)APG00565 (65.58% identity, 74.08% similarity) APG00972 (64.43% identity,74.23% similarity) APG06431 202 203 MTX 100 100 WP_058211730.1 (100.00%identity, 100.00% similarity) APG00410 (98.75% identity, 99.37%similarity) W7BXB5_9LIST (26.91% identity, 44.48% similarity)S4G0I5_ENTFL (26.71% identity, 47.18% similarity) AAB36655.1 (25.14%identity, 43.17% similarity) APG06630 204 205, 206 Cry42 50, 55, 60, 65,70, 75, 60, 65, 70, 75, 80, 85, APG00897 (94.07% identity, 96.35%similarity) 80, 85, 90, 95, 96, 97, 90, 95, 96, 97, 98, 99 APG00044.1 -US_2016_0304898_A1-72 (46.26% 98, 99 identity, 61.23% similarity)Cry42Aa1 (46.23% identity, 56.57% similarity) APG06650 207 208 Cry32 90,95, 96, 97, 98, 99 95, 96, 97, 98, 99 Cry32Ca1 (88.58% identity, 92.34%similarity) APG00687 (82.88% identity, 89.26% similarity) APG00056 -US_2016_0311864_A1-1 (53.22% identity, 65.18% similarity) APG00430(53.17% identity, 63.39% similarity) APG03519 (52.74% identity, 65.03%similarity) APG05370 (52.13% identity, 64.41% similarity) APG00710(51.72% identity, 62.18% similarity) APG01329 (50.69% identity, 62.51%similarity) APG06739 (50.66% identity, 61.45% similarity) APG06690 209MTX 80, 85, 90, 95, 96, 97, 90, 95, 96, 97, 98, 99US_2013_0227743_A1-100 (78.84% identity, 87.03% 98, 99 similarity)J8YPM2_BACCE (78.16% identity, 86.69% similarity) APG08718 (78.16%identity, 86.69% similarity) APG08241 (78.16% identity, 85.67%similarity) APG07639 (77.18% identity, 85.23% similarity) APG02279(76.16% identity, 84.44% similarity) APG09682 (74.58% identity, 86.62%similarity) APG00036.2 - US_2016_0304898_A1-229 (74.50% identity, 84.11%similarity) APG01506 (74.42% identity, 83.72% similarity) APG00036 -US_2016_0304898_A1-60 (73.77% identity, 83.28% similarity) APG04720(72.79% identity, 82.62% similarity) APG04076 (72.37% identity, 84.21%similarity) US_2013_0227743_A1-60 (40.07% identity, 47.35% similarity)APG06739 210 211, 212 213 Cry32 65, 70, 75, 80, 85, 90, 75, 80, 85, 90,95, 96, US_2011_0203014-27 (63.81% identity, 73.89% 95, 96, 97, 98, 9997, 98, 99 similarity) APG05370 (61.77% identity, 71.90% similarity)APG00974 (61.71% identity, 71.71% similarity) AGP18052.1 (61.28%identity, 71.27% similarity) Cry32Ab1 (60.52% identity, 71.01%similarity) APG00054 - US_2016_0304898_A1-84 (59.92% identity, 69.20%similarity) APG00469 (59.73% identity, 71.81% similarity) APG00068 -US_2016_0177333_A1-35 (59.08% identity, 70.38% similarity) APG05930(58.86% identity, 69.35% similarity) APG00673 (58.71% identity, 68.99%similarity) APG07100 (54.04% identity, 66.74% similarity) APG03519(53.25% identity, 64.35% similarity) APG06768 214 MTX 30, 35, 40, 45,50, 55, 45, 50, 55, 60, 65, 70, US_2015_0047076_A1-12 (26.92% identity,42.95% 60, 65, 70, 75, 80, 85, 75, 80, 85, 90, 95, 96, similarity) 90,95, 96, 97, 98, 99 97, 98, 99 US_2015_0047076_A1-8 (25.25% identity,42.95% similarity) ANN35810.1 (24.44% identity, 41.80% similarity)Cry45Aa (23.35% identity, 38.02% similarity) APG06784 215 216 MTX 35,40, 45, 50, 55, 60, 55, 60, 65, 70, 75, 80, WP_065845806.1 (32.85%identity, 51.74% 65, 70, 75, 80, 85, 90, 85, 90, 95, 96, 97, 98,similarity) 95, 96, 97, 98, 99 99 SDX17518.1 (28.37% identity, 48.03%similarity) SDW46809.1 (27.53% identity, 46.35% similarity)WP_006918908.1 (26.69% identity, 47.80% similarity) APG06880 217 218 MTX25, 30, 35, 40, 45, 50, 40, 45, 50, 55, 60, 65, APG09455 (78.62%identity, 84.91% similarity) 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90,95, US_2012_0278954_A1-30 (23.26% identity, 37.79% 85, 90, 95, 96, 97,98, 96, 97, 98, 99 similarity) 99 APG06912 219 220, 221 MTX 40, 45, 50,55, 60, 65, 55, 60, 65, 70, 75, 80, APG05384 (78.19% identity, 84.70%similarity) 70, 75, 80, 85, 90, 95, 85, 90, 95, 96, 97, 98,US_2013_0227743_A1-114 (39.04% identity, 54.55% 96, 97, 98, 99 99similarity) AIK29697.1 (22.57% identity, 39.90% similarity)CA_2844913-111 (37.53% identity, 52.76% similarity) APG06921 222 Cry 30,35, 40, 45, 50, 55, 40, 45, 50, 55, 60, 65, APG00084.1 -US_2016_0311864_A1-229 (64.17% 60, 65, 70, 75, 80, 85, 70, 75, 80, 85,90, 95, identity, 77.24% similarity) 90, 95, 96, 97, 98, 99 96, 97, 98,99 APG00084.2 - US_2016_0311864_A1-16 (63.84% identity, 76.72%similarity) APG00084 - US_2016_0311864_A1-15 (63.24% identity, 76.12%similarity) APG00152 - US_2016_0311864_A1-36 (61.82% identity, 70.89%similarity) APG07954 (56.15% identity, 67.44% similarity) APG00164 -US_2016_0311864_A1-41 (54.72% identity, 69.87% similarity) APG00654(53.65% identity, 69.59% similarity) Cry8Da1 (27.01% identity, 38.14%similarity) APG06938 223 Cry 95, 96, 97, 98, 99 97, 98, 99WP_025141994.1 (92.62% identity, 96.4% similarity) C6VUR9_DYAFD (38.16%identity, 48.21% similarity) SFM63176.1 (37.50% identity, 54.44%similarity) WP_035054034.1 (30.69% identity, 45.19% similarity) APG06942224 225, 226 227 Cry32 95, 96, 97, 98, 99 96, 97, 98, 99US_2013_0227743_A1-48 (93.78% identity, 95.75% similarity) APG00912(55.50% identity, 70.79% similarity) AEH76820.1 (54.09% identity, 67.80%similarity) APG00222 - US_2016_0304898_A1-203 (53.36% identity, 67.48%similarity) US_2010_0298211_A1-8 (50.89% identity, 63.78% similarity)Cry32Ea1 (43.22% identity, 57.00% similarity) APG06995 228 Cry 40, 45,50, 55, 60, 65, 55, 60, 65, 70, 75, 80, APG00404 -US_2016_0311864_A1-169 (78.21% 70, 75, 80, 85, 90, 95, 85, 90, 95, 96,97, 98, identity, 82.78% similarity) 96, 97, 98, 99 99 WP_048536362.1(37.08% identity, 53.29% similarity) AGA40057.1 (33.12% identity, 47.8%similarity) WP_017762616.1 (31.05% identity, 46.9% similarity) APG07016229 230, 231 Cry32 55, 60, 65, 70, 75, 80, 65, 70, 75, 80, 85, 90,APG00469 (51.61% identity, 61.67% similarity) 85, 90, 95, 96, 97, 98,95, 96, 97, 98, 99 APG06739 (50.83% identity, 60.83% similarity) 99AGP18024.1 (50.58% identity, 61.64% similarity) Cry32Ab1 (50.29%identity, 60.21% similarity) APG00310 (50.07% identity, 61.36%similarity) APG07036 232 233 MTX 95, 96, 97, 98, 99 95, 96, 97, 98, 99APG02293 (94.17% identity, 94.79% similarity) WP_050845726.1 (93.25%identity, 94.48% similarity) WP_000823322.1 (92.64% identity, 93.87%similarity) WP_016084062.1 (91.10% identity, 93.56% similarity)WP_016085279.1 (91.10% identity, 92.64% similarity) APG04804 (66.87%identity, 77.30% similarity) APG04640 (66.56% identity, 78.83%similarity) APG06161 (66.26% identity, 78.83% similarity) APG07037 234235 Cry20 75, 80, 85, 90, 95, 96, 85, 90, 95, 96, 97, 98, Cry20Aa1(71.96% identity, 81.01% similarity) 97, 98, 99 99 APG00810 (51.17%identity, 62.69% similarity) APG07058 236 237 MTX 20, 25, 30, 35, 40,45, 30, 35, 40, 45, 50, 55, AGP17988.1 (15.22% identity, 25.36%similarity) 50, 55, 60, 65, 70, 75, 60, 65, 70, 75, 80, 85, 80, 85, 90,95, 96, 97, 90, 95, 96, 97, 98, 99 98, 99 APG07100 238 239 Cry32 65, 70,75, 80, 85, 90, 75, 80, 85, 90, 95, 96, AGP18053.1 (62.56% identity,74.21% similarity) 95, 96, 97, 98, 99 97, 98, 99 US_2011_0203014-19(58.43% identity, 70.34% similarity) Cry32Da1 (58.12% identity, 67.82%similarity) APG06739 (54.04% identity, 66.74% similarity) APG00469(53.99% identity, 66.27% similarity) APG00974 (53.73% identity, 66.24%similarity) APG05370 (53.36% identity, 66.57% similarity) APG00068 -US_2016_0177333_A1-35 (53.35% identity, 66.47% similarity) APG05930(53.23% identity, 65.13% similarity) APG00673 (52.80% identity, 64.29%similarity) APG00310 (52.55% identity, 65.54% similarity) APG01387(50.22% identity, 61.26% similarity) APG07396 240 MTX 60, 65, 70, 75,80, 85, 75, 80, 85, 90, 95, 96, APG00907.1 - US_2016_0311864_A1-388(57.28% 90, 95, 96, 97, 98, 99 97, 98, 99 identity, 71.21% similarity)APG00907 - US_2016_0311864_A1-225 (56.92% identity, 70.77% similarity)APG00332 (56.02% identity, 70.48% similarity) AGA40062.1 (55.73%identity, 71.83% similarity) APG00112 - US_2016_0311864_A1-23 (55.49%identity, 71.34% similarity) Cry64Aa1 (36.56% identity, 51.06%similarity) APG07518 241 242 Cry34 70, 75, 80, 85, 90, 95, 80, 85, 90,95, 96, 97, KDN80650.1 (65.70% identity, 78.49% similarity) 96, 97, 98,99 98, 99 WP_030263691.1 (52.63% identity, 57.31% similarity)WP_035875344.1 (48.84% identity, 58.14% similarity) Cry34Ac2 (23.76%identity, 38.67% similarity) APG07559 243 244, 245 Cry 90, 95, 96, 97,98, 99 90, 95, 96, 97, 98, 99 WP_052899882.1 (88.06% identity, 88.06%similarity) V6MF71_PROHU (23.63% identity, 37.29% similarity)B4F0T5_PROMH (23.58% identity, 38.15% similarity) KGA56885.1 (23.55%identity, 38.29% similarity) APG07655 246 MTX 80, 85, 90, 95, 96, 97,90, 95, 96, 97, 98, 99 APG00155 - US_2016_0311864_A1-40 (96.62% 98, 99identity, 98.65% similarity) APG03379 (81.88% identity, 88.93%similarity) APG01506 (81.42% identity, 88.18% similarity) APG00749(81.33% identity, 88.33% similarity) APG08241 (79.73% identity, 87.16%similarity) APG08589 (79.05% identity, 88.18% similarity) APG02638(78.93% identity, 87.96% similarity) APG00201.1 - US_2016_0304898_A1-194(78.45% identity, 86.20% similarity) APG00107 - US_2016_0311864_A1-21(78.38% identity, 87.50% similarity) J8YPM2_BACCE (78.04% identity,86.15% similarity) APG00201 - US_2016_0304898_A1-193 (76.64% identity,84.21% similarity) APG07672 247 248, 249 MTX 97, 98, 99 99 OJG59749.1(96.86% identity, 98.43% similarity) WP_062805258.1 (96.54% identity,96.54% similarity) R2QDC7_ENTMU (96.23% identity, 96.54% similarity)V5XPU0_ENTMU (96.23% identity, 96.54% similarity) APG07731 250 251 MTX100 100 KFD20795.1 (100.00% identity, 100.00% similarity) WP_006820002.1(98.07% identity, 98.07% similarity) WP_011306610.1 (46.98% identity,65.08% similarity) WP_048178060.1 (46.44% identity, 62.54% similarity)APG07748 252 253, 254 MTX 85, 90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99APG00129 - US_2016_0304898_A1-158 (85.35% 99 identity, 87.41%similarity) APG00129.1 - US_2016_0304898_A1-159 (85.13% identity, 87.41%similarity) APG06372 (82.61% identity, 86.73% similarity) WP_061657790.1(82.15% identity, 86.27% similarity) W8YCZ9_BACTU (82.15% identity,86.04% similarity) APG00681 (58.28% identity, 71.30% similarity)APG03484 (54.89% identity, 68.89% similarity) APG00304 (53.20% identity,65.07% similarity) APG00366 (50.68% identity, 64.55% similarity)APG07783 255 256 MTX 35, 40, 45, 50, 55, 60, 45, 50, 55, 60, 65, 70,WP_036654376.1 (30.62% identity, 44.10% 65, 70, 75, 80, 85, 90, 75, 80,85, 90, 95, 96, similarity) 95, 96, 97, 98, 99 97, 98, 99 W2E623_9BACL(29.55% identity, 42.90% similarity) A0A015NB99_BACTU (28.53% identity,41.58% similarity) CA_2753491-78 (27.45% identity, 43.42% similarity)APG07787 257 Cry 40, 45, 50, 55, 60, 65, 55, 60, 65, 70, 75, 80,WP_025141994.1 (39.55% identity, 54.29% 70, 75, 80, 85, 90, 95, 85, 90,95, 96, 97, 98, similarity) 96, 97, 98, 99 99 SFM63176.1 (32.92%identity, 48.62% similarity) C6VUR9_DYAFD (27.26% identity, 37.23%similarity) WP_015811311.1 (12.60% identity, 17.57% similarity) APG07790258 259, 260 Vip4 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 95,WP_002193627.1 (52.81% identity, 66.95% 85, 90, 95, 96, 97, 98, 96, 97,98, 99 similarity) 99 APG09984 (51.82% identity, 65.92% similarity)APG00106 - US_2016_0304898_A1-134 (51.50% identity, 65.45% similarity)APG04931 (51.38% identity, 65.84% similarity) US_2013_0227743_A1_130(50.05% identity, 64.41% similarity) Vip4Aa1 (49.95% identity, 65.48%similarity) APG07803 261 262 Cry 45, 50, 55, 60, 65, 70, 60, 65, 70, 75,80, 85, APG06253 (88.97% identity, 93.37% similarity) 75, 80, 85, 90,95, 96, 90, 95, 96, 97, 98, 99 APG00188 - US_2016_0311864_A1-57 (88.11%97, 98, 99 identity, 92.36% similarity) APG00007 - US_2016_0304898_A1-11(73.01% identity, 82.49% similarity) APG01780 (72.78% identity, 80.76%similarity) APG00007.1 - US_2016_0304898_A1-12 (72.63% identity, 81.8%similarity) APG01915 (70.57% identity, 80.58% similarity) Cry32Ca1(43.12% identity, 55.74% similarity) APG07818 263 264 Bin 25, 30, 35,40, 45, 50, 40, 45, 50, 55, 60, 65, APG00798.1 - US_2016_0311864_A1-381(95.29% 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 95, identity, 96.77%similarity) 85, 90, 95, 96, 97, 98, 96, 97, 98, 99 APG00798 -US_2016_0311864_A1-219 (93.20% 99 identity, 94.66% similarity)APG00212.1 - US_2016_0311864_A1-259 (91.04% identity, 95.52% similarity)APG00619 - US_2016_0311864_A1-199 (90.57% identity, 94.04% similarity)APG00212 - US_2016_0311864_A1-75 (89.05% identity, 93.43% similarity)APG00600 - US_2016_0311864_A1-197 (86.53% identity, 92.77% similarity)APG00592 - US_2016_0311864_A1-195 (84.91% identity, 90.02% similarity)APG04176 (82.38% identity, 89.33% similarity) APG05715 (82.34% identity,89.55% similarity) APG02445 (81.33% identity, 88.21% similarity)Cry35Ad1 (20.81% identity, 36.24% similarity) APG07857 265 MACPF 70, 75,80, 85, 90, 95, 85, 90, 95, 96, 97, 98, WP_012145116.1 (69.25% identity,84.52% 96, 97, 98, 99 99 similarity) WP_019961352.1 (33.65% identity,51.73% similarity) WP_012039071.1 (32.27% identity, 50.60% similarity)WP_046018755.1 (29.57% identity, 46.69% similarity) APG07860 266 267 Cry40, 45, 50, 55, 60, 65, 55, 60, 65, 70, 75, 80, APG00153.1 -US_2016_0311864_A1-244 (84.27% 70, 75, 80, 85, 90, 95, 85, 90, 95, 96,97, 98, identity, 88.86% similarity) 96, 97, 98, 99 99 APG00153 -US_2016_0311864_A1-38 (80.48% identity, 84.86% similarity) APG00153.2 -US_2016_0311864_A1-245 (75.62% identity, 78.37% similarity) APG09376(53.90% identity, 64.62% similarity) APG06528 (53.38% identity, 64.62%similarity) APG05660 (52.01% identity, 62.53% similarity) APG00116 -US_2016_0311864_A1-25 (51.94% identity, 62.67% similarity) Cry20Ba2(38.29% identity, 53.03% similarity) APG07866 268 Cry 45, 50, 55, 60,65, 70, 60, 65, 70, 75, 80, 85, APG07224 (54.17% identity, 63.27%similarity) 75, 80, 85, 90, 95, 96, 90, 95, 96, 97, 98, 99WP_044307385.1 (42.90% identity, 56.31% 97, 98, 99 similarity)US_2013_0227743_A1-200 (36.27% identity, 49.17% similarity) APG00401 -US_2016_0311864_A1-168 (33.28% identity, 49.63% similarity) Cry73Aa(19.46% identity, 30.37% similarity) APG07870 269 270, 271 Cry32 90, 95,96, 97, 98, 99 95, 96, 97, 98, 99 AGA40054.1 (88.65% identity, 91.49%similarity) US_2011_0203014-23 (84.37% identity, 88.97% similarity)U5KRS1_BACTU (84.29% identity, 88.97% similarity) APG01329 (82.97%identity, 88.14% similarity) APG00710 (80.73% identity, 86.31%similarity) APG00058 - US_2016_0311864_A1-3 (79.86% identity, 85.78%similarity) APG00430 (76.48% identity, 83.00% similarity) APG01387(74.81% identity, 81.03% similarity) APG00056 - US_2016_0311864_A1-1(67.24% identity, 77.59% similarity) Cry32Da1 (52.77% identity, 64.60%similarity) APG00469 (52.72% identity, 64.19% similarity) APG03519(52.64% identity, 64.83% similarity) APG05370 (51.28% identity, 62.78%similarity) APG07961 272 Cyt 45, 50, 55, 60, 65, 70, 60, 65, 70, 75, 80,85, APG00177.1 - US_2016_0311864_A1-248 (89.58% 75, 80, 85, 90, 95, 96,90, 95, 96, 97, 98, 99 identity, 93.33% similarity) 97, 98, 99 APG08230(88.05% identity, 90.84% similarity) APG00177 - US_2016_0311864_A1-52(85.66% identity, 89.24% similarity) APG00121.1 - US_2016_0311864_A1-238(83.33% identity, 91.67% similarity) APG00121 - US_2016_0311864_A1-29(72.99% identity, 80.29% similarity) APG04725 (60.58% identity, 78.42%similarity) APG00128 - US_2016_0304898_A1-157 (59.35% identity, 73.17%similarity) APG00126 - US_2016_0304898_A1-153 (57.31% identity, 70.36%similarity) APG00437 (56.25% identity, 70.31% similarity) APG08631(54.37% identity, 67.3% similarity) Cyt2Ca1 (42.75% identity, 57.25%similarity) APG07983 273 MTX 30, 35, 40, 45, 50, 55, 45, 50, 55, 60, 65,70, APG00598 (28.16% identity, 43.04% similarity) 60, 65, 70, 75, 80,85, 75, 80, 85, 90, 95, 96, APG00236 (27.74% identity, 41.44%similarity) 90, 95, 96, 97, 98, 99 97, 98, 99 APG09455 (27.05% identity,43.47% similarity) APG01996 (26.40% identity, 41.25% similarity)US_2015_0047076_A1-6 (26.33% identity, 41.67% similarity) APG08039 274275 MTX 90, 95, 96, 97, 98, 99 95, 96, 97, 98, 99 WP_034120444.1 (86.35%identity, 90.59% similarity) WP_032896431.1 (66.67% identity, 74.65%similarity) WP_057721649.1 (48.60% identity, 65.89% similarity)WP_065936110.1 (48.60% identity, 64.25% similarity) APG08065 276 277Cry32 35, 40, 45, 50, 55, 60, 45, 50, 55, 60, 65, 70, J8N566_BACCE(33.11% identity, 41.66% similarity) 65, 70, 75, 80, 85, 90, 75, 80, 85,90, 95, 96, AGA40055.1 (32.63% identity, 40.90% similarity) 95, 96, 97,98, 99 97, 98, 99 AGP18045.1 (29.58% identity, 36.61% similarity)Cry32Ea1 (28.75% identity, 35.85% similarity) APG08088 278 279 MTX 85,90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99 APG00006.2 -US_2016_0304898_A1-228 (95.02% 99 identity, 96.01% similarity)APG00006 - US_2016_0304898_A1-9 (94.43% identity, 95.41% similarity)APG00006.1 - US_2016_0304898_A1-10 (91.03% identity, 91.69% similarity)APG02279 (81.64% identity, 87.54% similarity) APG00036 -US_2016_0304898_A1-60 (81.27% identity, 87.96% similarity) APG00749(80.07% identity, 88.37% similarity) J8YPM2_BACCE (80.00% identity,87.33% similarity) APG04720 (79.93% identity, 86.96% similarity)APG03379 (79.40% identity, 88.70% similarity) APG00201 -US_2016_0304898_A1-193 (78.62% identity, 87.50% similarity) APG02638(77.81% identity, 87.42% similarity) APG09682 (77.67% identity, 88.00%similarity) APG08108 280 MTX 60, 65, 70, 75, 80, 85, 80, 85, 90, 95, 96,97, APG01037 (89.37% identity, 91.34% similarity) 90, 95, 96, 97, 98, 9998, 99 APG00623 (88.14% identity, 91.30% similarity) APG00556 (80.29%identity, 83.94% similarity) CA_2905444-2 (58.08% identity, 75.77%similarity) WP_063896378.1 (54.55% identity, 71.15% similarity)WP_069512762.1 (52.61% identity, 68.28% similarity) Cry46Ab (33.66%identity, 50.33% similarity) APG08139 281 Cyt 100 100 WP_043159001.1(100.00% identity, 100.00% similarity) APG00462 - US_2016_0311864_A1-183(84.92% identity, 92.86% similarity) WP_017413134.1 (80.24% identity,86.56% similarity) Cyt1Aa6 (34.05% identity, 47.67% similarity) APG08174282 283, 284 Cry 40, 45, 50, 55, 60, 65, 55, 60, 65, 70, 75, 80,APG09573 (90.92% identity, 94.29% similarity) 70, 75, 80, 85, 90, 95,85, 90, 95, 96, 97, 98, AGP18058.1 (38.69% identity, 51.96% similarity)96, 97, 98, 99 99 J8N566_BACCE (38.66% identity, 53.62% similarity)AGP18037.1 (38.17% identity, 52.91% similarity) Cry32Ab1 (34.16%identity, 50.94% similarity) APG08230 285 286 Cyt 45, 50, 55, 60, 65,70, 60, 65, 70, 75, 80, 85, APG00177 - US_2016_0311864_A1-52 (95.60% 75,80, 85, 90, 95, 96, 90, 95, 96, 97, 98, 99 identity, 98.00% similarity)97, 98, 99 APG00177.1 - US_2016_0311864_A1-248 (92.40% identity, 94.00%similarity) APG07961 (88.05% identity, 90.84% similarity) APG00177.2 -US_2016_0311864_A1-53 (85.20% identity, 87.60% similarity) APG00121 -US_2016_0311864_A1-29 (69.34% identity, 79.56% similarity) APG04725(57.14% identity, 75.79% similarity) APG00126 - US_2016_0304898_A1-153(56.18% identity, 74.10% similarity) APG00128 - US_2016_0304898_A1-157(55.86% identity, 69.53% similarity) APG00437 (54.41% identity, 71.26%similarity) APG08631 (52.81% identity, 68.91% similarity) Cyt2Aa2(41.45% identity, 59.64% similarity) APG08278 287 288, 289 MTX 80, 85,90, 95, 96, 97, 90, 95, 96, 97, 98, 99 APG00268 (92.82% identity, 96.28%similarity) 98, 99 APG00393 (90.96% identity, 95.48% similarity)APG00764 (90.69% identity, 94.95% similarity) APG00322 (90.16% identity,94.15% similarity) APG00481 (89.92% identity, 94.43% similarity)APG00635 - US_2016_0311864_A1-201 (88.30% identity, 92.29% similarity)APG00635.1 - US_2016_0311864_A1-369 (85.37% identity, 88.56% similarity)APG00020 - US_2016_0304898_A1-33 (81.91% identity, 89.10% similarity)APG01231 (78.80% identity, 84.29% similarity) WP_071770709.1 (78.48%identity, 85.30% similarity) APG08307 290 291 MTX 30, 35, 40, 45, 50,55, 40, 45, 50, 55, 60, 65, APG04807 (51.20% identity, 65.87%similarity) 60, 65, 70, 75, 80, 85, 70, 75, 80, 85, 90, 95,US_2013_0227743_A1-114 (25.48% identity, 36.30% 90, 95, 96, 97, 98, 9996, 97, 98, 99 similarity) AGP17988.1 (14.31% identity, 22.86%similarity) APG08350 292 293 Cry41 75, 80, 85, 90, 95, 96, 85, 90, 95,96, 97, 98, WP_001070417.1 (70.95% identity, 80.09% 97, 98, 99 99similarity) Cry41Ba2 (68.95% identity, 77.84% similarity) APG08396 294295, 296 Cry 30, 35, 40, 45, 50, 55, 45, 50, 55, 60, 65, 70, APG00189 -US_2016_0311864_A1-59 (97.77% 60, 65, 70, 75, 80, 85, 75, 80, 85, 90,95, 96, identity, 98.09% similarity) 90, 95, 96, 97, 98, 99 97, 98, 99APG00189.1 - US_2016_0311864_A1-60 (96.33% identity, 96.65% similarity)APG00189.2 - US_2016_0311864_A1-61 (96.01% identity, 96.33% similarity)APG00087 - US_2016_0177333_A1-47 (89.51% identity, 93.16% similarity)Cry1Ib4 (27.00% identity, 40.05% similarity) APG08461 297 Cyt 30, 35,40, 45, 50, 55, 50, 55, 60, 65, 70, 75, SFX65039.1 (26.45% identity,46.69% similarity) 60, 65, 70, 75, 80, 85, 80, 85, 90, 95, 96, 97,SFX65069.1 (25.58% identity, 43.80% similarity) 90, 95, 96, 97, 98, 9998, 99 SFX65053.1 (22.87% identity, 43.02% similarity) APG08525 298 299MTX 35, 40, 45, 50, 55, 60, 55, 60, 65, 70, 75, 80, APG04793 (85.39%identity, 92.51% similarity) 65, 70, 75, 80, 85, 90, 85, 90, 95, 96, 97,98, APG03686 (83.90% identity, 92.51% similarity) 95, 96, 97, 98, 99 99APG07676 (83.52% identity, 92.51% similarity) APG03747 (83.15% identity,92.13% similarity) APG00014 - US_2016_0304898_A1-24 (50.74% identity,67.41% similarity) US_2012_0278954_A1-22 (34.42% identity, 50.36%similarity) WP_033694890.1 (34.07% identity, 45.71% similarity) AF316145(31.25% identity, 48.96% similarity) APG08589 300 MTX 80, 85, 90, 95,96, 97, 90, 95, 96, 97, 98, 99 APG00930 - US_2016_0311864_A1-397 (87.16%98, 99 identity, 92.91% similarity) APG00107 - US_2016_0311864_A1-21(86.82% identity, 93.24% similarity) APG01245 - US_2016_0311864_A1-398(86.49% identity, 93.92% similarity) APG08241 (83.45% identity, 92.23%similarity) APG01506 (80.41% identity, 90.20% similarity) APG07655(79.05% identity, 88.18% similarity) J8YPM2_BACCE (78.72% identity,89.19% similarity) APG03379 (76.85% identity, 86.58% similarity)APG09682 (76.77% identity, 87.88% similarity) APG00847 -US_2016_0304898_A1-223 (76.32% identity, 85.53% similarity) APG08631 301302 Cyt 90, 95, 96, 97, 98, 99 95, 96, 97, 98, 99 APG00126 -US_2016_0304898_A1-153 (92.80% identity, 93.20% similarity) APG00437(91.20% identity, 94.00% similarity) APG00126.1 - US_2016_0304898_A1-154(90.40% identity, 90.80% similarity) WP_016110460.1 (88.00% identity,91.20% similarity) APG04725 (75.60% identity, 85.20% similarity)APG00121 - US_2016_0311864_A1-29 (55.40% identity, 68.71% similarity)APG07961 (54.37% identity, 67.30% similarity) APG00177 -US_2016_0311864_A1-52 (53.18% identity, 69.29% similarity) APG08230(52.81% identity, 68.91% similarity) APG00128 - US_2016_0304898_A1-157(52.69% identity, 67.69% similarity) Cyt2Ca1 (37.83% identity, 52.43%similarity) APG08657 303 304 Cry69 80, 85, 90, 95, 96, 97, 85, 90, 95,96, 97, 98, 99 K0G0L7_BACTU (77.37% identity, 84.36% 98, 99 similarity)WP_001075209.1 (76.92% identity, 83.90% similarity) Cry69Aa1 (74.11%identity, 81.02% similarity) APG00786 (65.49% identity, 76.17%similarity) APG02067 (63.83% identity, 74.05% similarity) APG00662(63.47% identity, 73.66% similarity) APG00079 - US_2016_0177333_A1-43(59.64% identity, 71.19% similarity) APG00059 - US_2016_0177333_A1-27(57.87% identity, 69.72% similarity) APG08665 305 306, 307 Cry 40, 45,50, 55, 60, 65, 55, 60, 65, 70, 75, 80, US_2013_0227743_A1-192 (36.69%identity, 50.63% 70, 75, 80, 85, 90, 95, 85, 90, 95, 96, 97, 98,similarity) 96, 97, 98, 99 99 AGA40064.1 (26.23% identity, 41.90%similarity) US_8461421_B2-100 (24.01% identity, 37.94% similarity)Cry70Ba1 (22.87% identity, 39.20% similarity) APG08693 308 309 MTX 45,50, 55, 60, 65, 70, 55, 60, 65, 70, 75, 80, AGA40042.1 (43.09% identity,53.59% similarity) 75, 80, 85, 90, 95, 96, 85, 90, 95, 96, 97, 98,AGA40043.1 (39.17% identity, 51.39% similarity) 97, 98, 99 99US_8829279_B2-11 (31.25% identity, 45.65% similarity) SDZ23421.1 (29.60%identity, 43.68% similarity) APG08817 310 311, 312 MTX 60, 65, 70, 75,80, 85, 70, 75, 80, 85, 90, 95, AGP18007.1 (56.62% identity, 69.61%similarity) 90, 95, 96, 97, 98, 99 96, 97, 98, 99 SDW56810.1 (35.75%identity, 51.81% similarity) SDW71198.1 (32.79% identity, 51.49%similarity) Cry55Aa1 (19.47% identity, 36.54% similarity) APG08898 313314 MTX 35, 40, 45, 50, 55, 60, 50, 55, 60, 65, 70, 75, AGC39300.1(34.04% identity, 48.46% similarity) 65, 70, 75, 80, 85, 90, 80, 85, 90,95, 96, 97, WP_043924590.1 (32.62% identity, 46.10% 95, 96, 97, 98, 9998, 99 similarity) AGP17979.1 (31.54% identity, 46.94% similarity)Cry55Aa1 (20.80% identity, 37.35% similarity) APG08919 315 316 MTX 45,50, 55, 60, 65, 70, 55, 60, 65, 70, 75, 80, EJL23000.1 (41.14% identity,54.27% similarity) 75, 80, 85, 90, 95, 96, 85, 90, 95, 96, 97, 98,WP_035088343.1 (35.12% identity, 50.00% 97, 98, 99 99 similarity)WP_035074455.1 (34.77% identity, 49.59% similarity) ABK64066.1 (29.31%identity, 43.87% similarity) APG08931 317 318 Cry 90, 95, 96, 97, 98, 9995, 96, 97, 98, 99 APG00206 - US_2016_0304898_A1-199 (95.58% identity,97.63% similarity) WP_048536348.1 (85.83% identity, 91.99% similarity)APG00206.1 - US_2016_0304898_A1-200 (66.43% identity, 67.66% similarity)Cry70Ba1 (19.49% identity, 31.19% similarity) APG09084 319 MTX 50, 55,60, 65, 70, 75, 65, 70, 75, 80, 85, 90, AGA40062.1 (47.81% identity,62.19% similarity) 80, 85, 90, 95, 96, 97, 95, 96, 97, 98, 99 AGA40061.1(42.24% identity, 56.03% similarity) 98, 99 Cry64Aa1 (32.54% identity,47.46% similarity) APG09232 320 321 MTX 35, 40, 45, 50, 55, 60, 55, 60,65, 70, 75, 80, APG02168 (76.88% identity, 85.84% similarity) 65, 70,75, 80, 85, 90, 85, 90, 95, 96, 97, 98, WP_065845806.1 (34.49% identity,49.57% 95, 96, 97, 98, 99 99 similarity) SDW46809.1 (30.70% identity,50.42% similarity) SDX17518.1 (28.02% identity, 45.33% similarity)WP_006918908.1 (23.38% identity, 42.25% similarity) APG09234 322 323VIP/MTX 20, 25, 30, 35, 40, 45, 35, 40, 45, 50, 55, 60, WP_069716196.1(18.74% identity, 30.87% 50, 55, 60, 65, 70, 75, 65, 70, 75, 80, 85, 90,similarity) 80, 85, 90, 95, 96, 97, 95, 96, 97, 98, 99 WP_069716192 1(17 69% identity 29 29% 98, 99 similarity) WP_068123920.1 (17.57%identity, 28.86% similarity) Vip1Aa3 (15.02% identity, 23.67%similarity) APG09296 324 325 Vip3 20, 25, 30, 35, 40, 45, 30, 35, 40,45, 50, 55, APG00074 - US_2016_0311864_A1-14 (56.98% 50, 55, 60, 65, 70,75, 60, 65, 70, 75, 80, 85, identity, 59.28% similarity) 80, 85, 90, 95,96, 97, 90, 95, 96, 97, 98, 99 APG000741 - US_2016_0311864_A1-228(56.32% 98, 99 identity, 58.62% similarity) APG00077 -US_2016_0304898_A1-99 (27.62% identity, 33.58% similarity) Vip3Bb3(16.26% identity, 26.21% similarity) APG09413 326 MTX 25, 30, 35, 40,45, 50, 30, 35, 40, 45, 50, 55, AGA40042.1 (20.29% identity, 29.12%similarity) 55, 60, 65, 70, 75, 80, 60, 65, 70, 75, 80, 85, AGA40043.1(20.29% identity, 28.82% similarity) 85, 90, 95, 96, 97, 98, 90, 95, 96,97, 98, 99 J7XTF7_BACCE (16.29% identity, 27.80% similarity) 99WP_065486138.1 (15.92% identity, 24.58% similarity) APG09484 327 328 MTX95, 96, 97, 98, 99 96, 97, 98, 99 WP_069927276.1 (91.95% identity,95.21% similarity) WP_069932919.1 (91.08% identity, 94.31% similarity)WP_030570374.1 (79.50% identity, 82.57% similarity) WP_058043677.1(72.78% identity, 83.36% similarity) APG09543 329 330 Cry 40, 45, 50,55, 60, 65, 55, 60, 65, 70, 75, 80, US_2013_0227743_A1-152 (36.69%identity, 52.22% 70, 75, 80, 85, 90, 95, 85, 90, 95, 96, 97, 98,similarity) 96, 97, 98, 99 99 WP_048536363.1 (27.09% identity, 41.74%similarity) US_2013_0227743_A1-202 (26.79% identity, 42.26% similarity)Cry3Ca1 (14.58% identity, 22.89% similarity) APG09545 331 332 Vip4 90,95, 96, 97, 98, 99 95, 96, 97, 98, 99 SCC38903.1 (87.01% identity,92.21% similarity) WP_048561796.1 (75.05% identity, 84.69% similarity)SCL96146.1 (74.95% identity, 84.29% similarity) APG04931 (59.26%identity, 70.37% similarity) Vip4Aa1 (53.92% identity, 69.8% similarity)APG09573 333 334, 335 Cry32 40, 45, 50, 55, 60, 65, 55, 60, 65, 70, 75,80, APG08174 (90.92% identity, 94.29% similarity) 70, 75, 80, 85, 90,95, 85, 90, 95, 96, 97, 98, AGP18058.1 (38.12% identity, 51.48%similarity) 96, 97, 98, 99 99 WP_029439076.1 (38.11% identity, 54.27%similarity) J8N566_BACCE (37.35% identity, 51.66% similarity) Cry32Aa1(34.45% identity, 50.41% similarity) APG09589 336 Bin 65, 70, 75, 80,85, 90, 80, 85, 90, 95, 96, 97, ELQ83808.1 (64.01% identity, 75.16%similarity) 95, 96, 97, 98, 99 98, 99 KOT92468.1 (64.01% identity,75.16% similarity) WP_030594506.1 (63.69% identity, 75.48% similarity)Cry34Ab1 (14.89% identity, 21.68% similarity) APG09630 337 338 Cry 50,55, 60, 65, 70, 75, 60, 65, 70, 75, 80, 85, APG00723 -US_2016_0311864_A1-209 (92.11% 80, 85, 90, 95, 96, 97, 90, 95, 96, 97,98, 99 identity, 94.27% similarity) 98, 99 APG00723.1 -US_2016_0311864_A1-375 (86.53% identity, 88.54% similarity)WP_061139970.1 (46.97% identity, 56.56% similarity) APG00232 -US_2016_0311864_A1-87 (36.06% identity, 46.76% similarity) APG09682 339MTX 80, 85, 90, 95, 96, 97, 90, 95, 96, 97, 98, 99 APG00201.1 -US_2016_0304898_A1-194 (83.84% 98, 99 identity, 89.90% similarity)APG00201 - US_2016_0304898_A1-193 (81.91% identity, 87.83% similarity)APG00847 - US_2016_0304898_A1-223 (81.58% identity, 87.83% similarity)APG03379 (81.54% identity, 88.26% similarity) APG00749 (79.93% identity,85.86% similarity) APG07655 (79.19% identity, 86.91% similarity)APG02279 (78.76% identity, 87.58% similarity) J8YPM2_BACCE (78.45%identity, 89.23% similarity) APG08088 (77.67% identity, 88.00%similarity) APG00006 - US_2016_0304898_A1-9 (77.45% identity, 87.25%similarity) APG08589 (76.77% identity, 87.88% similarity) APG09864 340341 MTX 25, 30, 35, 40, 45, 50, 40, 45, 50, 55, 60, 65, APG04807 (31.78%identity, 49.07% similarity) 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90,95, APG07058 (30.31% identity, 46.46% similarity) 85, 90, 95, 96, 97,98, 96, 97, 98, 99 APG00940 (28.61% identity, 46.81% similarity) 99APG08307 (26.61% identity, 45.87% similarity) APG01044 (25.18% identity,39.43% similarity) APG00156 (24.09% identity, 37.42% similarity)APG02531 (22.97% identity, 36.43% similarity) US_2013_0227743_A1-114(22.85% identity, 38.46% similarity) APG09877 342 Cry44 60, 65, 70, 75,80, 85, 70, 75, 80, 85, 90, 95, Cry44Aa (55.40% identity, 69.49%similarity) 90, 95, 96, 97, 98, 99 96, 97, 98, 99 APG09936 343 344 Cry95, 96, 97, 98, 99 97, 98, 99 WP_049738340.1 (94.64% identity, 96.50%similarity) APG05978 (93.61% identity, 94.52% similarity) SEE67477.1(36.79% identity, 58.24% similarity) WP_036155538.1 (31.08% identity,50.53% similarity) Cry19Ba1 (15.76% identity, 25.82% similarity)APG09947 345 346 Cry 30, 35, 40, 45, 50, 55, 45, 50, 55, 60, 65, 70,AGA40057.1 (26.26% identity, 40.36% similarity) 60, 65, 70, 75, 80, 85,75, 80, 85, 90, 95, 96, AGA40058.1 (25.86% identity, 42.59% similarity)90, 95, 96, 97, 98, 99 97, 98, 99 US20130227743A1_202 (25.07% identity,40.36% similarity) WP_048536362.1 (24.66% identity, 37.14% similarity)APG09984 347 Vip4 70, 75, 80, 85, 90, 95, 80, 85, 90, 95, 96, 97,APG00106 - US_2016_0304898_A1-134 (73.45% 96, 97, 98, 99 98, 99identity, 82.55% similarity) WP_002193627.1 (69.93% identity, 79.07%similarity) SCC34829.1 (67.35% identity, 76.31% similarity) APG04931(55.58% identity, 69.35% similarity) Vip4Aa1 (51.91% identity, 66.97%similarity) APG07790 (51.82% identity, 65.92% similarity) APG00905.0 348349 Bin 91, 92, 93, 94, 95, 96, 94, 95, 96, 97, 98, 99, APG07818.0(92.31% identity, 95.04% similarity) 97, 98, 99, 100 100 APG00619.0US_2016_0311864_A1-199 (90.82% identity, 93.8% similarity) APG00798.0US_2016_0311864_A1-219 (89.81% identity, 93.45% similarity) APG00212.0US_2016_0311864_A1-75 (84.71% identity, 91.26% similarity) APG00306.0US_2016_0366881_A1-3 (83.37% identity, 90.57% similarity) APG00592.0US_2016_0311864_A1-195 (83.25% identity, 88.35% similarity) APG00600.0US_2016_0311864_A1-197 (82.38% identity, 89.58% similarity) APG04176.0(82.18% identity, 89.6% similarity) WP_070144216.1 (81.68% identity,88.86% similarity) APG05715.0 (81.17% identity, 88.26% similarity)APG00806.0 (80.69% identity, 87.62% similarity) APG02445.0 (80.2%identity, 87.53% similarity) APG02585.0 350 351 Vip4 55, 60, 65, 70, 75,80, 70, 75, 80, 85, 90, 91, APG01474.0 (96.62% identity, 98.05%similarity) 85, 90, 91, 92, 93, 94, 92, 93, 94, 95, 96, 97,CA_2844913-129 (52.92% identity, 68.25% 95, 96, 97, 98, 99, 100 98, 99,100 similarity) CA_2844913-130 (52.92% identity, 68.25% similarity)WP_016123960.1 (52.79% identity, 66.87% similarity) AGT29561.1 (50.45%identity, 67.77% similarity) US_2016_0339078_A1-29674 (48.9% identity,68.53% similarity) APG04931.0 (48.85% identity, 66.3% similarity)SCC34829.1 (48.74% identity, 65.39% similarity) APG09984.0 (48.64%identity, 64.82% similarity) APG00106.0 US_2016_0304898_A1-134 (47.73%identity, 65.09% similarity) APG07790.0 (47.56% identity, 61.64%similarity) APG07577.0 (47.34% identity, 61.7% similarity)US_2016_0339078_A1-28464 (46.76% identity, 62.77% similarity)WP_048561796.1 (46.37% identity, 63.99% similarity) WP_053512697.1(46.18% identity, 63.89% similarity) SCL96146.1 (46.08% identity, 63.79%similarity) SCC38903.1 (45.2% identity, 62.35% similarity) APG03000.0352 Cry 98, 99, 100 99, 100 APG00189.0 US_2016_0311864_A1-59 (97.29%identity, 98.25% similarity) APG08396.0 (96.33% identity, 97.61%similarity) APG00087.0 US_2016_0177333_A1-47 (89.51% identity, 93.64%similarity) APG00179.0 US_2016_0304898_A1-186 (35.11% identity, 53.24%similarity) US_2016_0017363_A1-27 (35.11% identity, 53.24% similarity)US_2016_0017363_A1-28 (33.78% identity, 49.18% similarity)US_2016_0017363_A1-29 (33.63% identity, 49.03% similarity)US_2016_0017363_A1-30 (33.48% identity, 48.88% similarity)US_2016_0017363_A1-31 (33.03% identity, 48.13% similarity) APG06630.0(30.19% identity, 47.35% similarity) APG03995.0 353 354 MTX 95, 96, 97,98, 99, 100 98, 99, 100 APG00268.0 US_2016_0355842_A1-45 (94.68%identity, 97.34% similarity) APG00646.0 US_2016_0355842_A1-201 (94.68%identity, 97.34% similarity) APG00481.0 US_2016_0355842_A1-103 (92.57%identity, 95.49% similarity) APG08278.0 (91.22% identity, 95.21%similarity) APG00322.0 US_2016_0355842_A1-59 (90.43% identity, 93.35%similarity) APG00393.0 US_2016_0355842_A1-76 (89.1% identity, 93.35%similarity) APG00764.0 US_2016_0355842_A1-168 (88.83% identity, 93.09%similarity) APG00959.0 US_2016_0355842_A1-194 (87.5% identity, 92.02%similarity) APG00635.0 US_2016_0311864_A1-201 (85.9% identity, 90.16%similarity) APG00597.0 US_2016_0355842_A1-139 (85.49% identity, 90.5%similarity) APG04196.0 355 356 MTX 40, 45, 50, 55, 60, 65, 60, 65, 70,75, 80, 85, APG06784.0 (93.96% identity, 96.68% similarity) 70, 75, 80,85, 90, 91, 90, 91, 92, 93, 94, 95, APG07682.0 (37.13% identity, 56.43%similarity) 92, 93, 94, 95, 96, 97, 96, 97, 98, 99, 100 APG00444.0US_2016_0355842_A1-93 (35.99% 98, 99, 100 identity, 57.82% similarity)APG00441.0 US_2016_0355842_A1-91 (35.99% identity, 57.52% similarity)APG00833.0 US_2016_0355842_A1-175 (35.84% identity, 56.36% similarity)APG00143.0 US_2016_0355842_A1-4 (34.99% identity, 56.56% similarity)WP_065845806.1 (32.94% identity, 52.48% similarity) APG03227.0 (32.26%identity, 53.67% similarity) APG03867.0 (30.61% identity, 52.77%similarity) APG01087.0 (29.08% identity, 50.74% similarity) SDX17518.1(28.93% identity, 48.6% similarity) APG06001.0 (28.2% identity, 44.65%similarity) SDW46809.1 (27.81% identity, 46.35% similarity)WP_006918908.1 (27.65% identity, 47.94% similarity) APG05497.0 357 358MTX 96, 97, 98, 99, 100 99, 100 APG00049.0 US_2016_0304898_A1-78 (95.25%identity, 98.64% similarity) WP_000730625.1 (94.24% identity, 96.95%similarity) APG03746.0 (93.9% identity, 97.29% similarity) EEM56710.1(90.88% identity, 93.81% similarity) WP_008180054.1 (54.67% identity,69.67% similarity) WP_016099228.1 (52.61% identity, 69.61% similarity)WP_000790613.1 (52.1% identity, 66.34% similarity) WP_000163136.1(50.94% identity, 66.04% similarity) WP_001036192.1 (49.53% identity,65.3% similarity) US_8461415_B2-31 (48.93% identity, 65.44% similarity)APG00495.0 (48.93% identity, 62.69% similarity) WP_065212007.1 (48.9%identity, 65.93% similarity) WP_050822474.1 (48.9% identity, 65.62%similarity) APG00013.0 US_2016_0304898_A1-22 (48.58% identity, 65.62%similarity) APG00693.0 (48.33% identity, 65.05% similarity) SFM27417.1(48.14% identity, 64.29% similarity) APG00484.0 US_2016_0355842_A1-105(46.86% identity, 61.64% similarity) APG00719.0 US_2016_0355842_A1-164(46.23% identity, 64.78% similarity) APG00234.0 US_2016_0304898_A1-207(45.99% identity, 61.72% similarity) US_8829279_B2-38 (45.28% identity,60.91% similarity) APG06291.0 359 360 MTX 96, 97, 98, 99, 100 97, 98,99, 100 WP_071770709.1 (96.13% identity, 97.51% similarity) APG00704.0US_2016_0355842_A1-161 (95.32% identity, 96.69% similarity) APG01231.0(93.94% identity, 95.59% similarity) APG00020.0 US_2016_0304898_A1-33(83.47% identity, 89.53% similarity) APG00253.0 US_2016_0355842_A1-39(82.92% identity, 88.71% similarity) APG01508.0 (82.37% identity, 89.53%similarity) APG00764.0 US_2016_0355842_A1-168 (81.36% identity, 86.61%similarity) APG03995.0 (80.58% identity, 86.88% similarity) APG00959.0US_2016_0355842_A1-194 (80.31% identity, 84.78% similarity) APG00418.0US_2016_0355842_A1-84 (79.89% identity, 86.23% similarity) APG00268.0US_2016_0355842_A1-45 (79.79% identity, 86.09% similarity) APG00393.0US_2016_0355842_A1-76 (79.47% identity, 85.0% similarity) APG08278.0(79.27% identity, 86.09% similarity) APG00646.0 US_2016_0355842_A1-201(79.27% identity, 85.83% similarity) APG00322.0 US_2016_0355842_A1-59(79.0% identity, 84.51% similarity) APG00481.0 US_2016_0355842_A1-103(78.27% identity, 84.03% similarity) APG00635.0 US_2016_0311864_A1-201(77.95% identity, 84.78% similarity) APG00448.0 US_2016_0366881_A1-70(77.87% identity, 84.97% similarity) APG00597.0 US_2016_0355842_A1-139(77.08% identity, 82.81% similarity) WP_002166885.1 (75.26% identity,80.53% similarity) APG06371.0 361 362 MTX 92, 93, 94, 95, 96, 97, 95,96, 97, 98, 99, 100 APG04477.0 (94.94% identity, 96.73% similarity) 98,99, 100 APG00569.0 US_2016_0355842_A1-129 (91.37% identity, 93.75%similarity) APG00938.0 US_2016_0355842_A1-190 (88.39% identity, 94.35%similarity) APG00563.0 US_2016_0355842_A1-127 (82.74% identity, 88.39%similarity) APG09055.0 (82.14% identity, 90.18% similarity) APG00794.0US_2016_0355842_A1-171 (79.53% identity, 87.54% similarity) APG00387.0US_2016_0366881_A1-45 (62.06% identity, 75.59% similarity) APG00590.0US_2016_0311864_A1-193 (59.18% identity, 74.93% similarity) APG00146.0US_2016_0304898_A1-166 (58.28% identity, 71.89% similarity)WP_000794514.1 (57.52% identity, 71.39% similarity) US_8318900_B2-78(56.98% identity, 70.64% similarity) ANN35530.1 (55.59% identity, 70.29%similarity) ANN35739.1 (55.33% identity, 71.6% similarity) APG07577.0363 364 Vip4 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 91, APG07790.0(97.92% identity, 98.86% similarity) 85, 90, 91, 92, 93, 94, 92, 93, 94,95, 96, 97, US_2016_0339078_A1-28464 (52.43% identity, 95, 96, 97, 98,99, 100 98, 99, 100 66.76% similarity) APG09984.0 (51.73% identity,65.64% similarity) APG04931.0 (51.38% identity, 65.75% similarity)APG00106.0 US_2016_0304898_A1-134 (51.36% identity, 65.39% similarity)CA_2844913-129 (49.81% identity, 64.35% similarity) CA_2844913-130(49.81% identity, 64.35% similarity) SCC34829.1 (49.58% identity, 62.7%similarity) US_2016_0339078_A1-29674 (49.5% identity, 64.68% similarity)AGT29561.1 (48.5% identity, 62.19% similarity) WP_016123960.1 (48.48%identity, 61.8% similarity) APG01474.0 (48.19% identity, 62.25%similarity) WP_048561796.1 (48.09% identity, 63.61% similarity)WP_053512697.1 (48.09% identity, 63.52% similarity)US_2016_0339078_A1-28124 (48.07% identity, 60.39% similarity) SCL96146.1(48.0% identity, 63.43% similarity) APG02585.0 (47.34% identity, 61.7%similarity) SCC38903.1 (46.53% identity, 62.04% similarity) APG09545.0(45.07% identity, 60.04% similarity) APG07648.0 365 366 MTX 5, 10, 15,20, 25, 30, 5, 10, 15, 20, 25, 30, APG06161.0 (92.02% identity, 97.55%similarity) 35, 40, 45, 50, 55, 60, 35, 40, 45, 50, 55, 60, APG04640.0(90.8% identity, 96.32% similarity) 65, 70, 75, 80, 85, 90, 65, 70, 75,80, 85, 90, WP_016099386.1 (86.2% identity, 92.02% similarity) 91, 92,93, 94, 95, 96, 91, 92, 93, 94, 95, 96, APG04804.0 (69.94% identity,84.66% similarity) 97, 98, 99, 100 97, 98, 99, 100 WP_016084062.1 (68.4%identity, 82.21% similarity) WP_016085044.1 (68.4% identity, 82.21%similarity) WP_016084449.1 (68.1% identity, 81.9% similarity)WP_050845726.1 (67.79% identity, 81.6% similarity) APG02293.0 (67.79%identity, 81.29% similarity) WP_000823322.1 (67.48% identity, 81.6%similarity) WP_016083901.1 (66.56% identity, 80.06% similarity)WP_016085279.1 (66.56% identity, 79.75% similarity) WP_016084436.1(65.95% identity, 79.45% similarity) APG07036.0 (65.64% identity, 77.91%similarity) APG09231.0 367 MTX 90, 91, 92, 93, 94, 95, 93, 94, 95, 96,97, 98, APG08589.0 (96.28% identity, 97.64% similarity) 96, 97, 98, 99,100 99, 100 APG00107.0 US_2016_0311864_A1-21 (86.82% identity, 92.91%similarity) APG00930.0 US_2016_0311864_A1-397 (86.15% identity, 92.23%similarity) APG01245.0 US_2016_0311864_A1-398 (85.81% identity, 92.57%similarity) APG08241.0 (81.82% identity, 91.58% similarity) APG07655.0(79.39% identity, 87.84% similarity) APG00155.0 US_2016_0311864_A1-40(79.05% identity, 87.5% similarity) APG07639.0 (78.79% identity, 88.89%similarity) APG01506.0 (78.72% identity, 88.85% similarity) APG08718.0(78.45% identity, 89.56% similarity) WP_000963933.1 (78.45% identity,89.56% similarity) CA_2844913-100 (78.04% identity, 89.53% similarity)APG02638.0 (77.26% identity, 87.29% similarity) APG00749.0 (77.0%identity, 86.0% similarity) APG03379.0 (76.85% identity, 86.24%similarity) APG08088.0 (75.92% identity, 85.62% similarity) APG02279.0(75.82% identity, 86.6% similarity) APG09682.0 (75.76% identity, 86.87%similarity) APG00847.0 US_2016_0304898_A1-223 (75.66% identity, 85.53%similarity) APG00201.0 US_2016_0304898_A1-193 (75.0% identity, 85.2%similarity) APG00923.0 368 369, 370 MTX 97, 98, 99, 100 99, 100WP_078185377.1 (98.26% identity, 99.01% similarity) APG06372.0 (98.01%identity, 98.76% similarity) KXY21848.1 (97.52% identity, 98.76%similarity) CDN39409.1 (97.27% identity, 98.51% similarity) APG02362.0(96.77% identity, 98.01% similarity) APG00129.0 US_2016_0304898_A1-158(96.53% identity, 98.51% similarity) APG02421.0 (95.86% identity, 96.84%similarity) APG02686.0 (95.86% identity, 96.84% similarity)WP_080448724.1 (95.86% identity, 96.84% similarity) APG04650.0 (95.78%identity, 98.26% similarity) APG02194.0 (95.78% identity, 98.01%similarity) WP_080685552.1 (95.62% identity, 96.59% similarity)APG08381.0 (93.43% identity, 95.62% similarity) OQR53340.1 (91.56%identity, 96.03% similarity) APG03337.0 (91.32% identity, 95.53%similarity) APG00941.0 371 372 Cry 90, 91, 92, 93, 94, 95, 92, 93, 94,95, 96, 97, APG05930.0 (95.2% identity, 96.38% similarity) 96, 97, 98,99, 100 98, 99, 100 APG00673.0 (89.69% identity, 91.83% similarity)ADK66923.1 (67.99% identity, 77.99% similarity) US_8318900_B2-82 (67.78%identity, 77.59% similarity) US_8461421_B2-91 (67.34% identity, 76.77%similarity) US_8461421_B2-98 (65.87% identity, 75.71% similarity)US_8759619_B2-19 (64.35% identity, 74.7% similarity) APG00974.0 (62.8%identity, 73.56% similarity) APG05370.0 (60.79% identity, 71.21%similarity) APG06739.0 (60.72% identity, 70.75% similarity) APG01202.0373 374 MTX 85, 90, 91, 92, 93, 94, 92, 93, 94, 95, 96, 97, APG08525.0(92.13% identity, 95.88% similarity) 95, 96, 97, 98, 99, 100 98, 99, 100APG04793.0 (83.52% identity, 91.39% similarity) APG03686.0 (82.77%identity, 91.39% similarity) APG04375.0 (82.29% identity, 89.67%similarity) OTX35276.1 (82.26% identity, 91.32% similarity) APG07676.0(82.02% identity, 91.01% similarity) OUB46342.1 (81.89% identity, 90.94%similarity) APG03747.0 (80.52% identity, 90.64% similarity) APG00014.0US_2016_0304898_A1-24 (50.75% identity, 66.79% similarity) APG04013.0(47.74% identity, 62.37% similarity) APG01809.0 375 376 Bin 60, 65, 70,75, 80, 85, 70, 75, 80, 85, 90, 91, APG06051.0 (91.67% identity, 93.27%similarity) 90, 91, 92, 93, 94, 95, 92, 93, 94, 95, 96, 97,WP_016093722.1 (79.67% identity, 81.06% 96, 97, 98, 99, 100 98, 99, 100similarity) APG00215.0 US_2016_0311864_A1-78 (59.33% identity, 66.57%similarity) OTW86911.1 (57.46% identity, 66.3% similarity) APG00880.0US_2016_0366881_A1-134 (54.57% identity, 63.99% similarity) APG00386.0US_2016_0311864_A1-158 (50.97% identity, 61.77% similarity)WP_002167240.1 (50.94% identity, 61.19% similarity) WP_048517129.1(46.41% identity, 57.18% similarity) WP_002090518.1 (46.13% identity,57.18% similarity) US_8829279_B2-4 (36.39% identity, 57.28% similarity)APG02194.0 377 378, 379 MTX 96, 97, 98, 99, 100 99, 100 APG06372.0(96.28% identity, 98.76% similarity) WP_078185377.1 (96.03% identity,98.51% similarity) APG00129.0 US_2016_0304898_A1-158 (95.78% identity,98.01% similarity) APG00923.0 (95.78% identity, 98.01% similarity)APG04650.0 (95.53% identity, 97.77% similarity) KXY21848.1 (95.29%identity, 98.26% similarity) CDN39409.1 (95.04% identity, 98.01%similarity) APG02362.0 (95.04% identity, 97.77% similarity) APG02421.0(94.16% identity, 96.35% similarity) APG02686.0 (93.67% identity, 96.35%similarity) WP_080448724.1 (93.67% identity, 96.35% similarity)WP_080685552.1 (93.43% identity, 96.11% similarity) OQR53340.1 (91.32%identity, 96.03% similarity) APG08381.0 (91.24% identity, 95.38%similarity) APG03337.0 (91.07% identity, 95.53% similarity) APG02362.0380 382, 381 MTX 96, 97, 98, 99, 100 99, 100 APG06372.0 (96.77%identity, 98.51% similarity) WP_078185377.1 (96.77% identity, 98.26%similarity) APG00923.0 (96.77% identity, 98.01% similarity) KXY21848.1(96.28% identity, 98.26% similarity) CDN39409.1 (96.03% identity, 98.01%similarity) APG00129.0 US_2016_0304898_A1-158 (95.78% identity, 98.01%similarity) APG02421.0 (95.38% identity, 96.11% similarity) APG04650.0(95.29% identity, 98.26% similarity) APG02194.0 (95.04% identity, 97.77%similarity) APG02686.0 (94.65% identity, 96.35% similarity)WP_080448724.1 (94.65% identity, 96.35% similarity) WP_080685552.1(94.4% identity, 96.11% similarity) APG08381.0 (94.15% identity, 96.1%similarity) OQR53340.1 (92.06% identity, 96.53% similarity) APG03337.0(91.81% identity, 96.03% similarity) APG02421.0 383 384, 385 MTX 95, 96,97, 98, 99, 100 97, 98, 99, 100 APG02686.0 (98.3% identity, 99.27%similarity) WP_080448724.1 (98.3% identity, 99.27% similarity)WP_080685552.1 (98.05% identity, 99.03% similarity) APG08381.0 (96.35%identity, 98.05% similarity) KXY21848.1 (96.11% identity, 97.32%similarity) WP_078185377.1 (96.11% identity, 97.32% similarity)APG06372.0 (95.86% identity, 97.08% similarity) CDN39409.1 (95.86%identity, 97.08% similarity) APG00923.0 (95.86% identity, 96.84%similarity) APG02362.0 (95.38% identity, 96.11% similarity) APG00129.0US_2016_0304898_A1-158 (94.4% identity, 96.35% similarity) APG04650.0(94.16% identity, 96.59% similarity) APG02194.0 (94.16% identity, 96.35%similarity) OQR53340.1 (90.02% identity, 94.4% similarity) APG02686.0386 387, 388 MTX 95, 96, 97, 98, 99, 100 97, 98, 99, 100 WP_080448724.1(100.0% identity, 100.0% similarity) WP_080685552.1 (99.76% identity,99.76% similarity) APG02421.0 (98.3% identity, 99.27% similarity)KXY21848.1 (97.81% identity, 98.05% similarity) CDN39409.1 (97.57%identity, 97.81% similarity) WP_078185377.1 (96.11% identity, 97.32%similarity) APG06372.0 (95.86% identity, 97.08% similarity) APG00923.0(95.86% identity, 96.84% similarity) APG08381.0 (95.62% identity, 97.81%similarity) APG02362.0 (94.65% identity, 96.35% similarity) APG00129.0US_2016_0304898_A1-158 (94.16% identity, 96.59% similarity) APG04650.0(93.92% identity, 96.84% similarity) APG02194.0 (93.67% identity, 96.35%similarity) APG03297.0 389 390, 391 MTX 92, 93, 94, 95, 96, 97, 94, 95,96, 97, 98, 99, WP_003290257.1 (99.67% identity, 100.0% 98, 99, 100 100similarity) APG03746.0 (91.86% identity, 94.14% similarity) APG00049.0US_2016_0304898_A1-78 (91.53% identity, 93.81% similarity) APG05497.0(90.88% identity, 93.81% similarity) WP_008180054.1 (52.41% identity,66.88% similarity) WP_016099228.1 (51.57% identity, 66.67% similarity)WP_000790613.1 (51.42% identity, 65.62% similarity) OUB62697.1 (51.1%identity, 67.19% similarity) OUB77698.1 (50.62% identity, 63.89%similarity) APG00495.0 (50.46% identity, 64.74% similarity) APG03337.0392 393 MTX 92, 93, 94, 95, 96, 97, 96, 97, 98, 99, 100 OQR53340.1(99.26% identity, 99.5% similarity) 98, 99, 100 APG04650.0 (91.81%identity, 96.28% similarity) APG06372.0 (91.81% identity, 96.28%similarity) APG02362.0 (91.81% identity, 96.03% similarity)WP_078185377.1 (91.56% identity, 96.03% similarity) APG00129.0US_2016_0304898_A1-158 (91.32% identity, 95.53% similarity) APG00923.0(91.32% identity, 95.53% similarity) KXY21848.1 (91.07% identity, 96.28%similarity) CDN39409.1 (91.07% identity, 96.03% similarity) APG02194.0(91.07% identity, 95.53% similarity) APG03352.0 394 395 MTX 93, 94, 95,96, 97, 98, 95, 96, 97, 98, 99, 100 APG00597.0 US_2016_0355842_A1-139(92.39% 99, 100 identity, 94.49% similarity) APG08278.0 (90.74%identity, 93.12% similarity) APG00764.0 US_2016_0355842_A1-168 (89.95%identity, 93.12% similarity) APG00393.0 US_2016_0355842_A1-76 (88.59%identity, 92.31% similarity) APG00481.0 US_2016_0355842_A1-103 (88.13%identity, 92.61% similarity) APG00268.0 US_2016_0355842_A1-45 (87.83%identity, 92.06% similarity) APG00322.0 US_2016_0355842_A1-59 (87.8%identity, 92.31% similarity) APG00635.0 US_2016_0311864_A1-201 (86.77%identity, 91.8% similarity) APG03995.0 (86.51% identity, 91.01%similarity) APG00959.0 US_2016_0355842_A1-194 (86.47% identity, 91.25%similarity) APG03422.0 396 MTX 90, 91, 92, 93, 94, 95, 91, 92, 93, 94,95, 96, APG06372.0 (90.41% identity, 91.55% similarity) 96, 97, 98, 99,100 97, 98, 99, 100 WP_078185377.1 (90.18% identity, 91.32% similarity)APG02421.0 (89.5% identity, 91.1% similarity) APG02686.0 (89.5%identity, 91.1% similarity) WP_080448724.1 (89.5% identity, 91.1%similarity) APG00923.0 (89.5% identity, 90.41% similarity)WP_080685552.1 (89.27% identity, 90.87% similarity) KXY21848.1 (89.04%identity, 90.64% similarity) CDN39409.1 (88.81% identity, 90.41%similarity) APG04650.0 (88.36% identity, 90.64% similarity) APG02194.0(88.36% identity, 90.41% similarity) APG02362.0 (88.36% identity, 90.18%similarity) APG03438.0 397 398 Cry 5, 10, 15, 20, 25, 30, 5, 10, 15, 20,25, 30, APG09824.0 (98.37% identity, 98.84% similarity) 35, 40, 45, 50,55, 60, 35, 40, 45, 50, 55, 60, APG05978.0 (96.12% identity, 96.8%similarity) 65, 70, 75, 80, 85, 90, 65, 70, 75, 80, 85, 90, APG04458.0(95.81% identity, 97.67% similarity) 91, 92, 93, 94, 95, 96, 91, 92, 93,94, 95, 96, KNB72291.1 (95.81% identity, 97.67% similarity) 97, 98, 99,100 97, 98, 99, 100 APG09936.0 (95.35% identity, 96.98% similarity)WP_084765780.1 (94.08% identity, 95.67% similarity) SEE67477.1 (37.75%identity, 58.65% similarity) WP_036155538.1 (30.51% identity, 50.21%similarity) EXX63903.1 (29.78% identity, 50.0% similarity) APG03891.0399 MTX 96, 97, 98, 99, 100 98, 99, 100 APG00601.0US_2016_0355842_A1-141 (95.36% identity, 96.43% similarity) APG00021.0US_2016_0304898_A1-35 (95.0% identity, 97.5% similarity) APG03249.0(95.0% identity, 96.07% similarity) APG00091.0 US_2016_0304898_A1-120(92.86% identity, 94.64% similarity) US_8461421_B2-74 (90.71% identity,95.36% similarity) APG00850.0 US_2016_0355842_A1-178 (77.82% identity,89.82% similarity) US_9567381_B2-384 (32.06% identity, 47.74%similarity) ANN35810.1 (31.72% identity, 46.55% similarity)US_8461421_B2-102 (31.4% identity, 46.08% similarity)US_2015_0047076_A1-10 (31.14% identity, 46.02% similarity) APG04251.0400 401 MTX 5, 10, 15, 20, 25, 30, 5, 10, 15, 20, 25, 30, WP_016085044.1(100.0% identity, 100.0% 35, 40, 45, 50, 55, 60, 35, 40, 45, 50, 55, 60,similarity) 65, 70, 75, 80, 85, 90, 65, 70, 75, 80, 85, 90,WP_016084062.1 (99.69% identity, 99.69% 91, 92, 93, 94, 95, 96, 91, 92,93, 94, 95, 96, similarity) 97, 98, 99, 100 97, 98, 99, 100WP_016084449.1 (99.39% identity, 99.69% similarity) APG05337.0 (97.24%identity, 98.47% similarity) WP_050845726.1 (97.24% identity, 98.47%similarity) OUB25269.1 (96.93% identity, 98.16% similarity) APG02293.0(96.32% identity, 98.16% similarity) WP_000823322.1 (96.32% identity,97.55% similarity) APG04806.0 (94.79% identity, 96.63% similarity)APG07936.0 (94.79% identity, 96.63% similarity) WP_016083901.1 (94.17%identity, 96.01% similarity) WP_016085279.1 (93.25% identity, 95.4%similarity) WP_016084436.1 (92.64% identity, 95.09% similarity)APG07036.0 (90.8% identity, 93.25% similarity) APG04458.0 402 403 Cry 5,10, 15, 20, 25, 30, 5, 10, 15, 20, 25, 30, KNB72291.1 (98.83% identity,99.3% similarity) 35, 40, 45, 50, 55, 60, 35, 40, 45, 50, 55, 60,WP_084765780.1 (97.03% identity, 97.26% 65, 70, 75, 80, 85, 90, 65, 70,75, 80, 85, 90, similarity) 91, 92, 93, 94, 95, 96, 91, 92, 93, 94, 95,96, APG03438.0 (95.81% identity, 97.67% similarity) 97, 98, 99, 100 97,98, 99, 100 APG09824.0 (95.35% identity, 97.21% similarity) APG09936.0(94.64% identity, 96.5% similarity) APG05978.0 (93.38% identity, 95.21%similarity) SEE67477.1 (37.16% identity, 58.33% similarity)WP_036155538.1 (30.23% identity, 50.11% similarity) EXX63903.1 (29.17%identity, 50.22% similarity) APG04650.0 404 405, 406 MTX 98, 99, 100 99,100 APG00129.0 US_2016_0304898_A1-158 (97.02% identity, 98.26%similarity) WP_078185377.1 (96.03% identity, 98.76% similarity)APG06372.0 (95.78% identity, 98.51% similarity) APG00923.0 (95.78%identity, 98.26% similarity) KXY21848.1 (95.53% identity, 98.76%similarity) CDN39409.1 (95.53% identity, 98.51% similarity) APG02194.0(95.53% identity, 97.77% similarity) APG02362.0 (95.29% identity, 98.26%similarity) APG02421.0 (94.16% identity, 96.59% similarity) APG02686.0(93.92% identity, 96.84% similarity) WP_080448724.1 (93.92% identity,96.84% similarity) WP_080685552.1 (93.92% identity, 96.59% similarity)OQR53340.1 (92.06% identity, 96.77% similarity) APG03337.0 (91.81%identity, 96.28% similarity) APG08381.0 (91.0% identity, 95.13%similarity) APG04806.0 407 408 MTX 5, 10, 15, 20, 25, 30, 5, 10, 15, 20,25, 30, APG05337.0 (96.93% identity, 98.16% similarity) 35, 40, 45, 50,55, 60, 35, 40, 45, 50, 55, 60, WP_050845726.1 (96.93% identity, 98.16%65, 70, 75, 80, 85, 90, 65, 70, 75, 80, 85, 90, similarity) 91, 92, 93,94, 95, 96, 91, 92, 93, 94, 95, 96, OUB25269.1 (96.63% identity, 97.85%similarity) 97, 98, 99, 100 97, 98, 99, 100 APG02293.0 (96.01% identity,97.24% similarity) WP_000823322.1 (96.01% identity, 97.24% similarity)WP_016084062.1 (95.09% identity, 96.93% similarity) WP_016084449.1(95.09% identity, 96.93% similarity) APG04251.0 (94.79% identity, 96.63%similarity) APG07936.0 (94.79% identity, 96.63% similarity)WP_016085044.1 (94.79% identity, 96.63% similarity) WP_016084436.1(94.79% identity, 96.01% similarity) WP_016083901.1 (93.87% identity,95.71% similarity) WP_016085279.1 (93.87% identity, 95.71% similarity)APG07036.0 (91.1% identity, 92.33% similarity) APG05226.0 409 410 MTX90, 91, 92, 93, 94, 95, 95, 96, 97, 98, 99, 100 WP_062673074.1 (98.79%identity, 99.09% 96, 97, 98, 99, 100 similarity) WP_079243808.1 (91.82%identity, 96.97% similarity) WP_047426867.1 (91.82% identity, 96.36%similarity) APG03072.0 (91.52% identity, 96.06% similarity)WP_076596488.1 (90.61% identity, 94.24% similarity) APG07574.0 (88.18%identity, 93.33% similarity) APG03185.0 (87.88% identity, 94.24%similarity) WP_077413348.1 (86.71% identity, 93.05% similarity)APG02518.0 (86.1% identity, 92.75% similarity) APG03114.0 (78.25%identity, 90.03% similarity) APG05337.0 411 412 MTX 5, 10, 15, 20, 25,30, 5, 10, 15, 20, 25, 30, WP_050845726.1 (99.39% identity, 100.0% 35,40, 45, 50, 55, 60, 35, 40, 45, 50, 55, 60, similarity) 65, 70, 75, 80,85, 90, 65, 70, 75, 80, 85, 90, OUB25269.1 (98.47% identity, 99.69%similarity) 91, 92, 93, 94, 95, 96, 91, 92, 93, 94, 95, 96,WP_000823322.1 (98.47% identity, 99.08% 97, 98, 99, 100 97, 98, 99, 100similarity) APG02293.0 (97.55% identity, 99.39% similarity)WP_016084062.1 (97.55% identity, 98.77% similarity) APG04251.0 (97.24%identity, 98.47% similarity) WP_016085044.1 (97.24% identity, 98.47%similarity) APG04806.0 (96.93% identity, 98.16% similarity) APG07936.0(96.93% identity, 98.16% similarity) WP_016084449.1 (96.63% identity,98.16% similarity) WP_016085279.1 (95.4% identity, 96.93% similarity)WP_016083901.1 (95.09% identity, 96.93% similarity) WP_016084436.1(94.79% identity, 96.63% similarity) APG07036.0 (92.94% identity, 94.17%similarity) APG06330.0 413 414, 415 Bin 95, 96, 97, 98, 99, 100 97, 98,99, 100 APG00118.0 US_2016_0311864_A1-28 (94.47% identity, 96.54%similarity) APG01790.0 (94.47% identity, 96.54% similarity) APG00454.0US_2016_0311864_A1-179 (90.78% identity, 95.62% similarity) APG00335.0US_2016_0366881_A1-18 (90.55% identity, 95.62% similarity) APG00223.0US_2016_0311864_A1-82 (90.55% identity, 95.39% similarity) APG00242.0US_2016_0311864_A1-93 (90.32% identity, 95.39% similarity) APG00913.0US_2016_0311864_A1-226 (88.25% identity, 91.57% similarity)WP_002187944.1 (87.1% identity, 88.48% similarity) WP_078212086.1(81.34% identity, 85.94% similarity) CA_2844913-10 (81.11% identity,86.64% similarity) APG06841.0 416 417 MTX 5, 10, 15, 20, 25, 30, 5, 10,15, 20, 25, 30, OUB72081.1 (97.86% identity, 99.08% similarity) 35, 40,45, 50, 55, 60, 35, 40, 45, 50, 55, 60, OUB84419.1 (95.41% identity,97.25% similarity) 65, 70, 75, 80, 85, 90, 65, 70, 75, 80, 85, 90,APG04640.0 (94.19% identity, 96.64% similarity) 91, 92, 93, 94, 95, 96,91, 92, 93, 94, 95, 96, APG06161.0 (88.07% identity, 93.27% similarity)97, 98, 99, 100 97, 98, 99, 100 APG07648.0 (85.32% identity, 92.97%similarity) OUA87853.1 (85.02% identity, 92.05% similarity) EOP79330.1(80.73% identity, 87.16% similarity) OUB80918.1 (79.82% identity, 90.52%similarity) APG04804.0 (70.03% identity, 83.18% similarity) APG04251.0(67.89% identity, 81.65% similarity) WP_016084062.1 (67.89% identity,81.65% similarity) WP_016085044.1 (67.89% identity, 81.65% similarity)APG07160.0 418 419, 420 MTX 5, 10, 15, 20, 25, 30, 5, 10, 15, 20, 25,30, WP_006675125.1 (91.67% identity, 91.67% 35, 40, 45, 50, 55, 60, 35,40, 45, 50, 55, 60, similarity) 65, 70, 75, 80, 85, 90, 65, 70, 75, 80,85, 90, APG02408.0 (91.05% identity, 95.06% similarity) 91, 92, 93, 94,95, 96, 91, 92, 93, 94, 95, 96, WP_019421057.1 (74.62% identity, 85.63%97, 98, 99, 100 97, 98, 99, 100 similarity) WP_006285537.1 (72.75%identity, 82.34% similarity) WP_016083901.1 (47.79% identity, 64.31%similarity) APG04804.0 (47.48% identity, 65.58% similarity) APG05337.0(47.2% identity, 64.31% similarity) WP_050845726.1 (47.2% identity,64.31% similarity) OUB25269.1 (47.2% identity, 64.01% similarity)APG02293.0 (47.2% identity, 63.72% similarity) APG07724.0 421 422 Cry75, 80, 85, 90, 91, 92, 85, 90, 91, 92, 93, 94, APG01780.0 (97.09%identity, 97.95% similarity) 93, 94, 95, 96, 97, 98, 95, 96, 97, 98, 99,100 APG07803.0 (73.0% identity, 81.24% similarity) 99, 100US_8461421_B2-68 (71.22% identity, 82.23% similarity) APG00007.0US_2016_0304898_A1-11 (71.14% identity, 82.31% similarity) APG06253.0(69.73% identity, 79.85% similarity) APG00188.0 US_2016_0311864_A1-57(69.44% identity, 79.48% similarity) APG01915.0 (64.5% identity, 75.34%similarity) APG00912.0 (45.51% identity, 58.6% similarity) AEH76820.1(45.11% identity, 58.72% similarity) CA_2844913-48 (44.83% identity,57.22% similarity) APG07936.0 423 424 MTX 5, 10, 15, 20, 25, 30, 5, 10,15, 20, 25, 30, WP_050845726.1 (97.24% identity, 98.47% 35, 40, 45, 50,55, 60, 35, 40, 45, 50, 55, 60, similarity) 65, 70, 75, 80, 85, 90, 65,70, 75, 80, 85, 90, APG05337.0 (96.93% identity, 98.16% similarity) 91,92, 93, 94, 95, 96, 91, 92, 93, 94, 95, 96, OUB25269.1 (96.32% identity,98.16% similarity) 97, 98, 99, 100 97, 98, 99, 100 WP_000823322.1(96.32% identity, 97.55% similarity) APG02293.0 (96.01% identity, 97.85%similarity) WP_016084062.1 (95.09% identity, 96.93% similarity)APG04251.0 (94.79% identity, 96.63% similarity) APG04806.0 (94.79%identity, 96.63% similarity) WP_016085044.1 (94.79% identity, 96.63%similarity) WP_016084449.1 (94.79% identity, 96.32% similarity)WP_016085279.1 (93.56% identity, 95.71% similarity) WP_016083901.1(92.94% identity, 96.01% similarity) WP_016084436.1 (92.94% identity,95.4% similarity) APG07036.0 (91.72% identity, 93.56% similarity)APG08381.0 425 426 MTX 92, 93, 94, 95, 96, 97, 95, 96, 97, 98, 99, 100APG02421.0 (96.35% identity, 98.05% similarity) 98, 99, 100 APG02686.0(95.62% identity, 97.81% similarity) WP_080448724.1 (95.62% identity,97.81% similarity) WP_080685552.1 (95.38% identity, 97.57% similarity)APG02362.0 (94.15% identity, 96.1% similarity) APG06372.0 (93.43%identity, 96.11% similarity) KXY21848.1 (93.43% identity, 95.86%similarity) WP_078185377.1 (93.43% identity, 95.86% similarity)APG00923.0 (93.43% identity, 95.62% similarity) CDN39409.1 (93.19%identity, 95.62% similarity) APG02194.0 (91.24% identity, 95.38%similarity) APG00129.0 US_2016_0304898_A1-158 (91.24% identity, 94.89%similarity) APG04650.0 (91.0% identity, 95.13% similarity) APG09755.0427 428 MTX 97, 98, 99, 100 99, 100 APG00704.0 US_2016_0355842_A1-161(96.42% identity, 98.62% similarity) APG06291.0 (93.66% identity, 96.69%similarity) APG01231.0 (93.39% identity, 96.14% similarity)WP_071770709.1 (93.11% identity, 95.32% similarity) APG00020.0US_2016_0304898_A1-33 (81.59% identity, 88.46% similarity) APG00253.0US_2016_0355842_A1-39 (80.77% identity, 87.09% similarity) APG01508.0(80.49% identity, 87.91% similarity) APG00764.0 US_2016_0355842_A1-168(78.8% identity, 84.03% similarity) APG03995.0 (78.27% identity, 85.34%similarity) APG00418.0 US_2016_0355842_A1-84 (77.75% identity, 84.62%similarity) APG09824.0 429 430 Cry 5, 10, 15, 20, 25, 30, 5, 10, 15, 20,25, 30, APG03438.0 (98.37% identity, 98.84% similarity) 35, 40, 45, 50,55, 60, 35, 40, 45, 50, 55, 60, APG05978.0 (96.35% identity, 97.03%similarity) 65, 70, 75, 80, 85, 90, 65, 70, 75, 80, 85, 90, APG04458.0(95.35% identity, 97.21% similarity) 91, 92, 93, 94, 95, 96, 91, 92, 93,94, 95, 96, KNB72291.1 (95.35% identity, 97.21% similarity) 97, 98, 99,100 97, 98, 99, 100 APG09936.0 (95.35% identity, 96.98% similarity)WP_084765780.1 (93.62% identity, 95.22% similarity) SEE67477.1 (37.75%identity, 58.65% similarity) WP_036155538.1 (31.57% identity, 50.42%similarity) EXX63903.1 (29.26% identity, 49.56% similarity) APG01515.0431 432 433 Cry 75, 80, 85, 90, 91, 92, 85, 90, 91, 92, 93, 94,APG07724.0 (93.39% identity, 95.51% similarity) 93, 94, 95, 96, 97, 98,95, 96, 97, 98, 99, 100 APG01780.0 (92.20% identity, 95.04% similarity)99, 100 APG07803.0 (75.97% identity, 82.66% similarity) APG00007.0US_2016_0304898_A1-11 (72.15% identity, 83.09% similarity) APG00188.0US_2016_0311864_A1-57 (71.72% identity, 80.83% similarity) APG06253.0(70.97% identity, 80.14% similarity) US_8461421_B2-68 (69.36% identity,81.38% similarity) APG01915.0 (63.28% identity, 74.81% similarity)WP_087949412.1 (45.93% identity, 59.19% similarity) APG00912.0US_2017_0175134_A1-175 (45.66% identity, 58.54% similarity) APG01949.0434 MTX 5, 10, 15, 20, 25, 30, 5, 10, 15, 20, 25, 30, APG05337.0 (98.16%identity, 99.39% similarity) 35, 40, 45, 50, 55, 60, 35, 40, 45, 50, 55,60, WP_050845726.1 (98.16% identity, 99.39% 65, 70, 75, 80, 85, 90, 65,70, 75, 80, 85, 90, similarity) 91, 92, 93, 94, 95, 96, 91, 92, 93, 94,95, 96, WP_086397429.1 (97.24% identity, 99.08% 97, 98, 99, 100 97, 98,99, 100 similarity) WP_000823322.1 (97.24% identity, 98.47% similarity)APG02293.0 (96.32% identity, 98.77% similarity) APG07936.0 (96.32%identity, 97.55% similarity) WP_016084062.1 (95.71% identity, 98.16%similarity) APG04806.0 (95.71% identity, 97.55% similarity) APG04251.0(95.40% identity, 97.85% similarity) WP_016085044.1 (95.40% identity,97.85% similarity) WP_016084449.1 (95.40% identity, 97.55% similarity)WP_016085279.1 (94.17% identity, 96.32% similarity) WP_016084436.1(93.56% identity, 96.01% similarity) WP_016083901.1 (93.25% identity,96.32% similarity) APG07036.0 (91.72% identity, 93.56% similarity)APG02436.0 435 436 MTX 96, 97, 98, 99, 100 97, 98, 99, 100 APG00322.0US_2016_0355842_A1-59 (95.20% identity, 96.27% similarity) APG00481.0US_2016_0355842_A1-103 (94.68% identity, 95.74% similarity) APG00268.0US_2016_0355842_A1-45 (94.41% identity, 96.01% similarity) APG03995.0(91.49% identity, 94.41% similarity) APG08278.0 (90.16% identity, 94.15%similarity) APG00646.0 US_2016_0355842_A1-201 (90.16% identity, 93.88%similarity) APG00635.0 US_2016_0311864_A1-201 (88.56% identity, 91.49%similarity) APG00393.0 US_2016_0355842_A1-76 (87.73% identity, 92.00%similarity) APG03352.0 (87.53% identity, 91.25% similarity) APG00764.0US_2016_0355842_A1-168 (86.97% identity, 91.49% similarity) APG02554.0437 MTX 85, 90, 91, 92, 93, 94, 90, 91, 92, 93, 94, 95, APG09682.0(95.62% identity, 98.65% similarity) 95, 96, 97, 98, 99, 100 96, 97, 98,99, 100 APG00201.0 US_2016_0304898_A1-193 (80.92% identity, 87.17%similarity) APG00847.0 US_2016_0304898_A1-223 (80.59% identity, 87.17%similarity) APG03379.0 (79.87% identity, 87.58% similarity) APG08241.0(79.80% identity, 88.55% similarity) APG07639.0 (79.80% identity, 88.22%similarity) APG02279.0 (79.41% identity, 87.25% similarity) APG08718.0(79.12% identity, 88.89% similarity) WP_000963933.1 (79.12% identity,88.89% similarity) CA_2844913-100 (79.12% identity, 88.55% similarity)APG02886.0 438 439 Cyt 85, 90, 91, 92, 93, 94, 90, 91, 92, 93, 94, 95,APG04725.0 (90.17% identity, 94.02% similarity) 95, 96, 97, 98, 99, 10096, 97, 98, 99, 100 APG00126.0 US_2016_0304898_A1-153 (82.08% identity,89.17% similarity) WP_016110460.1 (80.50% identity, 87.97% similarity)APG08631.0 (77.20% identity, 84.00% similarity) APG00437.0US_2016_0366881_A1-62 (77.20% identity, 82.80% similarity) APG07961.0(59.75% identity, 76.76% similarity) APG00177.0 US_2016_0311864_A1-52(55.95% identity, 73.81% similarity) APG08230.0 (55.95% identity, 73.81%similarity) WP_016110459.1 (55.56% identity, 71.60% similarity)APG00128.0 US_2016_0304898_A1-157 (55.56% identity, 71.19% similarity)APG03861.0 440 441, 442 MTX 35, 40, 45, 50, 55, 60, 50, 55, 60, 65, 70,75, APG07783.0 (97.59% identity, 98.49% similarity) 65, 70, 75, 80, 85,90, 80, 85, 90, 91, 92, 93, APG00276.0 US_2016_0355842_A1-47 (33.43% 91,92, 93, 94, 95, 96, 94, 95, 96, 97, 98, 99, identity, 47.67% similarity)97, 98, 99, 100 100 APG00545.0 US_2016_0355842_A1-122 (31.81% identity,46.42% similarity) APG00671.0 US_2016_0355842_A1-157 (29.39% identity,48.41% similarity) APG00362.0 US_2016_0366881_A1-33 (29.13% identity,43.98% similarity) WP_061885189.1 (28.12% identity, 44.03% similarity)APG00552.0 US_2016_0366881_A1-110 (27.87% identity, 41.26% similarity)WP_088118891.1 (27.84% identity, 44.03% similarity) APG04374.0 443 444,445, 446 Cry 90, 91, 92, 93, 94, 95, 95, 96, 97, 98, 99, 100 APG01644.0(91.05% identity, 94.30% similarity) 96, 97, 98, 99, 100 APG00110.0US_2016_0177333_A1-60 (89.77% identity, 94.65% similarity)US_8318900_B2-205 (62.10% identity, 74.32% similarity) APG00672.0US_2017_0175134_A1-91 (61.57% identity, 73.15% similarity) APG00045.0US_2016_0304898_A1-73 (61.09% identity, 71.09% similarity) APG01742.0(60.86% identity, 73.87% similarity) US_8318900_B2-69 (56.04% identity,68.00% similarity) EOO24729.1 (48.35% identity, 62.80% similarity)WP_082186915.1 (46.81% identity, 60.79% similarity) US_8318900_B2-207(41.80% identity, 51.66% similarity) APG04547.0 447 448 MTX 5, 10, 15,20, 25, 30, 5, 10, 15, 20, 25, 30, APG07648.0 (96.32% identity, 98.77%similarity) 35, 40, 45, 50, 55, 60, 35, 40, 45, 50, 55, 60, OUA87853.1(95.71% identity, 98.16% similarity) 65, 70, 75, 80, 85, 90, 65, 70, 75,80, 85, 90, APG05852.0 (94.17% identity, 97.55% similarity) 91, 92, 93,94, 95, 96, 91, 92, 93, 94, 95, 96, APG06161.0 (92.02% identity, 96.32%similarity) 97, 98, 99, 100 97, 98, 99, 100 APG04640.0 (90.80% identity,95.09% similarity) OUB84419.1 (89.26% identity, 94.48% similarity)OUB72081.1 (85.63% identity, 91.44% similarity) EOP79330.1 (85.58%identity, 91.10% similarity) APG06841.0 (85.32% identity, 91.74%similarity) WP_088048270.1 (80.37% identity, 91.41% similarity)APG05852.0 449 450 MTX 5, 10, 15, 20, 25, 30, 5, 10, 15, 20, 25, 30,OUA87853.1 (95.40% identity, 97.55% similarity) 35, 40, 45, 50, 55, 60,35, 40, 45, 50, 55, 60, APG04547.0 (94.17% identity, 97.55% similarity)65, 70, 75, 80, 85, 90, 65, 70, 75, 80, 85, 90, APG07648.0 (93.87%identity, 98.47% similarity) 91, 92, 93, 94, 95, 96, 91, 92, 93, 94, 95,96, APG06161.0 (92.94% identity, 97.55% similarity) 97, 98, 99, 100 97,98, 99, 100 APG04640.0 (90.49% identity, 96.32% similarity) OUB84419.1(89.57% identity, 96.32% similarity) OUB72081.1 (85.93% identity, 93.27%similarity) APG06841.0 (85.63% identity, 93.58% similarity) EOP79330.1(85.28% identity, 91.41% similarity) WP_088048270.1 (80.37% identity,92.33% similarity) APG06492.0 451 MTX 85, 90, 91, 92, 93, 94, 90, 91,92, 93, 94, 95, APG05804.0 (96.36% identity, 97.68% similarity) 95, 96,97, 98, 99, 100 96, 97, 98, 99, 100 US_9567381_B2-446 (84.26% identity,89.18% similarity) APG00450.0 US_2016_0366881_A1-72 (82.95% identity,86.23% similarity) APG00501.0 US_2016_0366881_A1-96 (72.46% identity,74.43% similarity) US_8461415_B2-57 (42.62% identity, 60.66% similarity)APG00987.0 US_2016_0366881_A1-223 (41.88% identity, 61.04% similarity)APG00346.0 US_2016_0366881_A1-24 (41.03% identity, 56.41% similarity)US_8461415_B2-56 (40.92% identity, 57.10% similarity) APG00697.0US_2016_0366881_A1-222 (40.06% identity, 55.45% similarity) APG00506.0US_2016_0366881_A1-102 (39.81% identity, 60.19% similarity) APG06587.0452 453 Cry 60, 65, 70, 75, 80, 85, 70, 75, 80, 85, 90, 91,WP_017762619.1 (91.09% identity, 95.29% 90, 91, 92, 93, 94, 95, 92, 93,94, 95, 96, 97, similarity) 96, 97, 98, 99, 100 98, 99, 100 APG01399.0(90.92% identity, 95.46% similarity) CA_2844913-205 (55.81% identity,67.94% similarity) CA_2844913-206 (55.65% identity, 67.77% similarity)WP_078205743.1 (51.61% identity, 64.47% similarity) APG00460.0US_2016_0366881_A1-75 (51.45% identity, 64.31% similarity) APG00626.0US_2016_0366881_A1-124 (37.09% identity, 50.15% similarity)WP_017762581.1 (35.48% identity, 49.76% similarity) APG00329.0US_2016_0355842_A1-62 (35.05% identity, 48.44% similarity) APG06997.0(34.47% identity, 50.93% similarity) APG07911.0 454 455 Bin 90, 91, 92,93, 94, 95, 93, 94, 95, 96, 97, 98, APG04176.0 (90.55% identity, 93.03%similarity) 96, 97, 98, 99, 100 99, 100 CA_2844913-146 (88.45% identity,92.38% similarity) APG05715.0 (88.31% identity, 92.04% similarity)APG02445.0 (88.31% identity, 91.54% similarity) APG00806.0US_2017_0175134_A1-167 (84.33% identity, 90.05% similarity)WP_070144216.1 (84.33% identity, 89.55% similarity) WP_074651503.1(83.66% identity, 90.35% similarity) APG00212.0 US_2016_0311864_A1-75(81.73% identity, 87.02% similarity) APG00905.0 (79.17% identity, 86.03%similarity) APG07818.0 (78.62% identity, 85.75% similarity) APG08051.0456 457, 458 MTX 90, 91, 92, 93, 94, 95, 95, 96, 97, 98, 99, 100APG02782.0 (96.32% identity, 98.16% similarity) 96, 97, 98, 99, 100APG02960.0 (86.81% identity, 94.17% similarity) APG04643.0 (86.50%identity, 94.48% similarity) APG08085.0 (83.13% identity, 88.96%similarity) APG00743.0 (81.29% identity, 88.34% similarity)WP_044444098.1 (81.29% identity, 88.34% similarity) APG02555.0 (81.29%identity, 88.04% similarity) WP_090995413.1 (75.84% identity, 84.10%similarity) WP_060749709.1 (75.54% identity, 85.32% similarity)APG04485.0 (51.78% identity, 65.38% similarity)

i. Classes of Pesticidal Proteins

The pesticidal proteins provided herein and the nucleotide sequencesencoding them are useful in methods for impacting pests. That is, thecompositions and methods of the invention find use in agriculture forcontrolling or killing pests, including pests of many crop plants. Thepesticidal proteins provided herein are toxin proteins from bacteria andexhibit activity against certain pests. The pesticidal proteins are fromseveral classes of toxins including Cry, Cyt, BIN, Mtx toxins. See, forexample, Table 1 for the specific protein classifications of the variousSEQ ID NOS provided herein. In addition, reference is made throughoutthis disclosure to Pfam database entries. The Pfam database is adatabase of protein families, each represented by multiple sequencealignments and a profile hidden Markov model. Finn et al. (2014) Nucl.Acid Res. Database Issue 42.D222-D230.

Bacillus thuringiensis (Bt) is a gram-positive bacterium that producesinsecticidal proteins as crystal inclusions during its sporulation phaseof growth. The proteinaceous inclusions of Bacillus thuringiensis (Bt)are called crystal proteins or S-endotoxins (or Cry proteins), which aretoxic to members of the class Insecta and other invertebrates.Similarly, Cyt proteins are parasporal inclusion proteins from Bt thatexhibits hemolytic (Cytolitic) activity or has obvious sequencesimilarity to a known Cyt protein. These toxins are highly specific totheir target organism, are innocuous to humans, vertebrates, and plants.

The structure of the Cry toxins reveals five conserved amino acidblocks, concentrated mainly in the center of the domain or at thejunction between the domains. The Cry toxin consists of three domains,each with a specific function. Domain I is a seven α-helix bundle inwhich a central helix is completely surrounded by six outer helices.This domain is implicated in channel formation in the membrane. DomainII appears as a triangular column of three anti-parallel β-sheets, whichare similar to antigen-binding regions of immunoglobulins. Domain IIIcontains anti-parallel β-strands in a β sandwich form. The N-terminalpart of the toxin protein is responsible for its toxicity andspecificity and contains five conserved regions. The C-terminal part isusually highly conserved and probably responsible for crystal formation.See, for example, U.S. Pat. No. 8,878,007.

Strains of B. thuringiensis show a wide range of specificity againstdifferent insect orders (Lepidoptera, Diptera, Coleoptera, Hymenoptera,Homoptera, Phthiraptera or Mallophaga, and Acari) and otherinvertebrates (Nemathelminthes, Platyhelminthes, and Sarocomastebrates).The cry proteins have been classified into groups based on toxicity tovarious insect and invertebrate groups. Generally, Cry I demonstratestoxicity to lepidopterans, Cry II to lepidopterans and dipterans, CryIIIto coleopterans, Cry IV to dipterans, and Cry V and Cry VI to nematodes.New Cry proteins can be identified and assigned to a Cry group based onamino acid identity. See, for example, Bravo, A. (1997) J. of Bacteriol.179:2793-2801; Bravo et al. (2013) Microb. Biolechnol. 6:17-26, hereinincorporated by reference.

Over 750 different cry gene sequences have been classified into 73groups (Cry1-Cry73), with new members of this gene family continuing tobe discovered (Crickmore el al. (2014); found on the world wide web atbtnomenclature.info/). The cry gene family consists of severalphylogenetically non-related protein families that may have differentmodes of action: the family of three-domain Cry toxins, the family ofmosquitocidal Cry toxins, the family of the binary-like toxins, and theCyt family of toxins (Bravo et al., 2005). Some Bt strains produceadditional insecticidal toxins, the VIP toxins. See, also, Cohen et al.(2011) J. Mol. Biol. 413:4-814; Crickmore et al. (2014) Bacillusthuringiensis toxin nomenclature, found on the world wide web atlifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/; Crickmore et al. (1988)Microbiol. Mol. Biol. Rev. 62: 807-813; Gill et al. (1992) Ann. Rev.Entomol. 37: 807-636; Goldbert et al. (1997) Appl. Environ. Microbiol.63:2716-2712; Knowles et al. (1992) Proc. R. Soc. Ser. B. 248: 1-7; Koniet al. (1994) Microbiology 140: 1869-1880; Lailak et al. (2013) Biochem.Biophys. Res. Commun. 435: 216-221; Lopez-Diaz el al. (2013) Environ.Microbiol. 15: 3030-3039; Perez et al. (2007) Cell. Microbiol. 9:2931-2937; Promdonkoy et al. (2003) Biochem. J. 374: 255-259; Rigden(2009) FEBS Lett. 583: 1555-1560; Schnepf et al. (1998) Microbiol. Mol.Biol. Rev. 62: 775-806; Soberon et al. (2013) Peptides 41: 87-93; Thieryel al. (1998) J. Am. Mosq. Control Assoc. 14: 472-476; Thomas et al.(1983) FEBS Lett. 154: 362-368; Wirth et al. (1997) Proc. Natl. Acad.Sci. U.S.A. 94: 10536-10540; Wirth et al (2005) Appl. Environ.Microbiol. 71: 185-189; and, Zhang et al. (2006) Biosci. Biotechnol.Biochem. 70: 2199-2204; each of which is herein incorporated byreference in their entirety.

Cyt designates a parasporal crystal inclusion protein from Bacillusthuringiensis with cytolytic activity, or a protein with sequencesimilarity to a known Cyt protein. (Crickmore et al. (1998) Microbiol.Mol. Biol. Rev. 62: 807-813). The gene is denoted by cyt. These proteinsare different in structure and activity from Cry proteins (Gill et al.(1992) Annu. Rev. Entomol. 37: 615-636). The Cyt toxins were firstdiscovered in B. thuringiensis subspecies israelensis (Goldberg et al.(1977) Mosq. News. 37: 355-358). There are 3 Cyt toxin familiesincluding 11 holotype toxins in the current nomenclature (Crickmore etal. (2014) Bacillus thuringiensis toxin nomenclature found on the worldwide web at lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/). The majorityof the B. thuringiensis isolates with cyt genes show activity againstdipteran insects (particularly mosquitoes and black flies), but thereare also cyt genes that have been described in B. thuringiensis strainstargeting lepidopteran or coleopteran insects (Guerchicoff et al. (1997)Appl. Environ. Microbiol. 63: 2716-2721).

The structure of Cyt2A, solved by X-ray crystallography, shows a singledomain where two outer layers of α-helix wrap around a mixed β-sheet.Further available crystal structures of Cyt toxins support a conservedα-β structural model with two α-helix hairpins flanking a β-sheet corecontaining seven to eight β-strands. (Cohen et al. (2011) J. Mol. Biot.413: 80 4-814) Mutagenic studies identified β-sheet residues as criticalfor toxicity, while mutations in the helical domains did not affecttoxicity (Adang et al.; Diversity of Bacillus thuringiensis CrystalToxins and Mechanism of Action. In: T. S. Dhadialla and S. S. Gill, eds,Advances in Insect Physiology, Vol. 47, Oxford: Academic Press, 2014,pp. 39-87.) The representative domain of the Cyt toxin is a δ-endotoxin,Bac_thur_toxin (Pfam PF01338).

There are multiple proposed models for the mode of action of Cyt toxins,and it is still an area of active investigation. Some Cyt proteins(Cyt1A) have been shown to require the presence of accessory proteinsfor crystallization. Cyt1A and Cyt2A protoxins are processed bydigestive proteases at the same sites in the N- and C-termini to astable toxin core. Cyt toxins then interact with non-saturated membranelipids, such as phosphatidylcholine, phosphatidylethanolamine, andsphingomyelin. For Cyt toxins, pore-formation and detergent-likemembrane disruption have been proposed as non-exclusive mechanisms; andit is generally accepted that both may occur depending on toxinconcentration, with lower concentrations favoring oligomeric pores andhigher concentrations leading to membrane breaks. (Butko (2003) Appl.Environ. Microbiol. 69: 2415-2422) In the pore-formation model, the Cyttoxin binds to the cell membrane, inducing the formation ofcation-selective channels in the membrane vesicles leading tocolloid-osmotic lysis of the cell. (Knowles et al. (1989) FEBS Lett.244: 259-262; Knowles et al. (1992) Proc. R. Soc. Ser. B. 248: 1-7 andPromdonkoy et al. (2003) Biochem. J. 374: 255-259). In the detergentmodel, there is a nonspecific aggregation of the toxin on the surface ofthe lipid bilayer leading to membrane disassembly and cell death. (Butko(2003) supra; Manceva et al. (2005) Biochem. 44: 589-597).

Multiple studies have shown synergistic activity between Cyt toxins andother B. thuringiensis toxins, particularly the Cry, Bin, and Mtxtoxins. This synergism has even been shown to overcome an insect'sresistance to the other toxin. (Wirth 1997, Wirth 2005, Thiery 1998,Zhang 2006) The Cyt synergistic effect for Cry toxins is proposed toinvolve Cyt1A binding to domain II of Cry toxins in solution or on themembrane plane to promote formation of a Cry toxin pre-pore oligomer.Formation of this oligomer is independent of the Cyt oligomerization,binding or insertion. (Lailak 2013, Perez 2007, Lopez-Diaz 2013)

A number of pesticidal proteins unrelated to the Cry proteins areproduced by some strains of B. thuringiensis and B. cereus duringvegetative growth (Estruch et al. (1996) Proc Natl Acad Sci USA93:5389-5394; Warren et al. (1994) WO 94/21795). These vegetativeinsecticidal proteins, or Vips, do not form parasporal crystal proteinsand are apparently secreted from the cell. The Vips are presentlyexcluded from the Cry protein nomenclature because they are notcrystal-forming proteins. The term VIP is a misnomer in the sense thatsome B. thuringiensis Cry proteins are also produced during vegetativegrowth as well as during the stationary and sporulation phases, mostnotably Cry3Aa. The location of the Vip genes in the B. thuringiensisgenome has been reported to reside on large plasmids that also encodecry genes (Mesrati et al. (2005) FEMS Microbiol. Lett. 244(2):353-8). Aweb-site for the nomenclature of Bt toxins can be found on the worldwide web at lifesci.sussex.ac.uk with the path“/home/Neil_Crickmore/Bt/” and at: “btnomenclature.info/”. See also,Schnepf et al. (1998) Microbiol. Mol. Biol. Rev. 62(3):775-806. Suchreferences are herein incorporated by reference.

To date four categories of Vips have been identified. Some Vip genesform binary two-component protein complexes; an “A” component is usuallythe “active” portion, and a “B” component is usually the “binding”portion. (Pfam pfam.xfam.org/family/PF03495). The Vip1 and Vip4 proteinsgenerally contain binary toxin B protein domains. Vip2 proteinsgenerally contain binary toxin A protein domains.

The Vip1 and Vip2 proteins are the two components of a binary toxin thatexhibits toxicity to coleopterans. Vip1Aa1 and Vip2Aa1 are very activeagainst corn rootworms, particularly Diabrotica virgifera and Diabroticalongicornis (Han et al. (1999) Nat. Struct. Biol. 6:932-936; Warren G W(1997) “Vegetative insecticidal proteins: novel proteins for control ofcorn pests” In: Carozzi N B, Koziel M (eds) Advances in insect control,the role of transgenic plants; Taylor & Francis Ltd, London, pp 109-21).The membrane-binding 95 kDa Vip1 multimer provides a pathway for the 52kDa vip2 ADP-ribosylase to enter the cytoplasm of target western cornrootworm cells (Warren (1997) supra). The NAD-dependentADP-ribosyltransferase Vip2 likely modifies monomeric actin at Arg177 toblock polymerization, leading to loss of the actin cytoskeleton andeventual cell death due to the rapid subunit ex-change within actinfilaments in vivo (Carlier M. F. (1990) Adv. Biophys. 26:51-73).

Like Cry toxins, activated Vip3A toxins are pore-forming proteinscapable of making stable ion channels in the membrane (Lee et al. (2003)Appl. Environ. Microbiol. 69:4648-4657). Vip3 proteins are activeagainst several major lepidopteran pests (Rang et al. (2005) Appl.Environ. Microbiol. 71(10):6276-6281; Bhalla et al. (2005) FEMSMicrobiol. Lett. 243:467-472; Estruch et al. (1998) WO 9844137; Estruchet al. (1996) Proc Natl Acad Sci USA 93:5389-5394; Selvapandiyan et al.(2001) Appl. Environ Microbiol. 67:5855-5858; Yu et al. (1997) Appl.Environ Microbiol. 63:532-536). Vip3A is active against Agrotis ipsilon,Spodoptera frugiperda, Spodoptera exigua, Heliothis virescens, andHelicoverpa zea (Warren et al. (1996) WO 96/10083; Estruch et al. (1996)Proc Natl Acad Sci USA 93:5389-5394). Like Cry toxins, Vip3A proteinsmust be activated by proteases prior to recognition at the surface ofthe midgut epithelium of specific membrane proteins different from thoserecognized by Cry toxins.

The MTX family of toxin proteins is characterized by the presence of aconserved domain, ETX_MTX2 (pfam 03318). Members of this family sharesequence homology with the mosquitocidal toxins Mtx2 and Mtx3 fromBacillus sphaericus, as well as with the epsilon toxin ETX fromClostridium perfringens (Cole et al. (2004) Nat. Struct. Mol. Biol. 11:797-8; Thanabalu et al. (1996) Gene 170:85-9). The MTX-like proteins arestructurally distinct from the three-domain Cry toxins, as they have anelongated and predominately β-sheet-based structure. However, similar tothe three-domain toxins, the MTX-like proteins are thought to form poresin the membranes of target cells (Adang et al. (2014) supra). Unlike thethree-domain Cry proteins, the MTX-like proteins are much smaller inlength, ranging from 267 amino acids (Cry23) to 340 amino acids (Cry15A.

To date, only 15 proteins belonging to the family of MTX-like toxinshave been assigned Cry names, making this a relatively small classcompared to the three-domain Cry family (Crickmore et al. (2014) supra;Adang et al. (2014) supra). The members of the MTX-like toxin familyinclude Cry15, Cry23, Cry33, Cry38, Cry45, Cry46, Cry51, Cry60A, Cry60B,and Cry64. This family exhibits a range of insecticidal activity,including activity against insect pests of the Lepidopteran andColeopteran orders. Some members of this family may form binarypartnerships with other proteins, which may or may not be required forinsecticidal activity.

Cry15 is a 34 kDA protein that was identified in Bacillus thuringiensisserovar thompsoni HD542; it occurs naturally in a crystal together withan unrelated protein of approximately 40 kDa. The gene encoding Cry15and its partner protein are arranged together in an operon. Cry15 alonehas been shown to have activity against lepidopteran insect pestsincluding Manduca sexta, Cydia pomonella, and Pieris rapae, with thepresence of the 40 kDA protein having been shown to increase activity ofCry15 only against C. pomonella (Brown K. and Whiteley H. (1992) J.Bacteriol. 174:549-557; Naimov et al. (2008) Appl. Environ. Microbiol.74:7145-7151). Further studies are needed to elucidate the function ofthe partner protein of Cry15. Similarly, Cry23 is a 29 kDA protein thathas been shown to have activity against the coleopteran pests Triboliumcastaneum and Popillia japonica together with its partner protein Cry37(Donovan et al. (2000) U.S. Pat. No. 6,063,756).

New members of the MTX-like family are continuing to be identified. AnETX_MTX toxin gene was recently identified in the genome of Bacillusthuringiensis serovar tolworthi strain Na205-3. This strain was found tobe toxic against the lepidpoteran pest Helicoverpa armigera, and it alsocontained homologs of Cry1, Cry11, Vip1, Vip2, and Vip3 (Palma et al.(2014) Genome Announc. 2(2): e00187-14. Published online Mar. 13, 2014at doi: 10.1128/genomeA.00187-14; PMCID: PMC3953196). Because theMTX-like proteins have a unique domain structure relative to thethree-domain Cry proteins, they are believed to possess a unique mode ofaction, thereby making them a valuable tool in insect control and thefight against insect resistance.

Bacterial cells produce large numbers of toxins with diverse specificityagainst host and non-host organisms. Large families of binary toxinshave been identified in numerous bacterial families, including toxinsthat have activity against insect pests. (Poopathi and Abidha (2010) J.Physiol. Path. 1(3): 22-38). Lysinibacillus sphaericus (Ls), formerlyBacillus sphaericus, (Ahmed et al. (2007) Int. J. Syst. Evol. Microbiol.57:1117-1125) is well-known as an insect biocontrol strain. Ls producesseveral insecticidal proteins, including the highly potent binarycomplex BinA/BinB. This binary complex forms a parasporal crystal in Lscells and has strong and specific activity against dipteran insects,specifically mosquitos. In some areas, insect resistance to existing Lsmosquitocidal strains has been reported. The discovery of new binarytoxins with different target specificity or the ability to overcomeinsect resistance is of significant interest.

The Ls binary insecticidal protein complex contains two majorpolypeptides, a 42 kDa polypeptide and a 51 kDa polypeptide, designatedBinA and BinB, respectively (Ahmed et al. (2007) supra). The twopolypeptides act synergistically to confer toxicity to their targets.Mode of action involves binding of the proteins to receptors in thelarval midgut. In some cases, the proteins are modified by proteasedigestion in the larval gut to produce activated forms. The BinBcomponent is thought to be involved in binding, while the BinA componentconfers toxicity (Nielsen-LeRoux et al. (2001) Appl. Environ. Microbiol.67(11):5049-5054). When cloned and expressed separately, the BinAcomponent is toxic to mosquito larvae, while the BinB component is not.However, co-administration of the proteins markedly increases toxicity(Nielsen-LeRoux et al. (2001) supra).

A small number of Bin protein homologs have been described frombacterial sources. Priest el al. (1997) Appl. Environ. Microbiol.63(4):1195-1198 describe a hybridization effort to identify new Lsstrains, although most of the genes they identified encoded proteinsidentical to the known BinA/BinB proteins. The BinA protein contains adefined conserved domain known as the Toxin 10 superfamily domain. Thistoxin domain was originally defined by its presence in BinA and BinB.The two proteins both have the domain, although the sequence similaritybetween BinA and BinB is limited in this region (<40%). The Cry49Aaprotein, which also has insecticidal activity, also has this domain(described below).

The Cry48Aa/Cry49Aa binary toxin of Ls has the ability to kill Culexquinquefasciatus mosquito larvae. These proteins are in a proteinstructural class that has some similarity to the Cry protein complex ofBacillus thuringiensis (Bt), a well-known insecticidal protein family.The Cry34/Cry35 binary toxin of Bt is also known to kill insects,including Western corn rootworm, a significant pest of corn. Cry34, ofwhich several variants have been identified, is a small (14 kDa)polypeptide, while Cry35 (also encoded by several variants) is a 44 kDapolypeptide. These proteins have some sequence homology with theBinA/BinB protein group and are thought to be evolutionarily related(Ellis et al. (2002) Appl. Environ. Microbiol. 68(3):1137-1145).

Phosphoinositide phospholipase C proteins (PI-PLC; alsophosphotidylinositol phospholipase C) are members of the broader groupof phospholipase C proteins. Many of these proteins play important rolesin signal transduction as part of normal cell physiology. Severalimportant bacterial toxins also contain domains with similarity to theseproteins (Titball, R. W. (1993) Microbiological Reviews. 57(2):347-366).Importantly, these proteins are implicated in signal amplificationduring intoxication of insect cells by Bt Cry proteins (Valaitis, A. P.(2008) Insect Biochemistry and Molecular Biology. 38: 611-618).

The PI-PLC toxin class occurs in Bacillus isolates, commonly seen inco-occurrence with homologs to other described toxin classes, such asBinary Toxins. This class of sequences has homology tophosphatidylinositol phosphodiesterases (also referred to asphosphatidylinositol-specific phospholipase C-PI-PLC). The crystalstructure and its active site were solved for B. cereus PI-PLC by Heinzet al (Heinz, et. al., (1995) The EMBO Journal. 14(16): 3855-3863). Theroles of the B. cereus PI-PLC active site amino acid residues incatalysis and substrate binding were investigated by Gassler et al usingsite-directed mutagenesis, kinetics, and crystal structure analysis(Gassler, et. al., (1997) Biochemistry. 36(42):12802-13).

These PI-PLC toxin proteins contain a PLC-like phosphodiesterase, TIMbeta/alpha-barrel domain (IPR017946) and/or a Phospholipase C,phosphatidylinositol-specific, X domain (IPR000909) (also referred to asthe PI-PLC X-box domain). We have also seen proteins with these domainsin combination with other typical Bacillus protein toxin domains. Thislist includes most commonly a lectin domain (IPR000772), a sugar-bindingdomain that can be present in one or more copies and is thought to bindcell membranes, as well as the Insecticidal crystal toxin (IPR008872)(also referred to as Toxin10 or P42), which is the defining domain ofthe Binary Toxin.

Previously, toxins of this PI-PLC class were defined in U.S. Pat. No.8,318,900 B2 SEQ ID NOs 30 (DNA) and 79 (amino acid), in U.S. PatentPublication No. 20110263488A1 SEQ ID NOs 8 (DNA) and 9 (amino acid), andin U.S. Pat. No. 8,461,421B2 SEQ ID NOs 3 (DNA) and 63 (amino acid).

Provided herein are pesticidal proteins from these classes of toxins.The pesticidal proteins are classified by their structure, homology toknown toxins and/or their pesticidal specificity.

ii. Variants and Fragments of Pesticidal Proteins and PolynucleotidesEncoding the Same

Pesticidal proteins or polypeptides of the invention include those setforth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51,52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69,70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87,88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118,119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132,133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146,147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160,161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174,175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216,217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230,231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258,259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272,273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286,287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300,301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314,315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328,329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342,343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356,357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398,399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412,413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440,441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454,455, 456, 457, and/or 458 and fragments and variants thereof. By“pesticidal toxin” or “pesticidal protein” or “pesticidal polypeptide”is intended a toxin or protein or polypeptide that has activity againstone or more pests, including, insects, fungi, nematodes, and the likesuch that the pest is killed or controlled.

An “isolated” or “purified” polypeptide or protein, or biologicallyactive portion thereof, is substantially or essentially free fromcomponents that normally accompany or interact with the polypeptide orprotein as found in its naturally occurring environment. Thus, anisolated or purified polypeptide or protein is substantially free ofother cellular material, or culture medium when produced by recombinanttechniques, or substantially free of chemical precursors or otherchemicals when chemically synthesized. A protein that is substantiallyfree of cellular material includes preparations of protein having lessthan about 30%, 20%, 10%, 5%, or 1% (by dry weight) of contaminatingprotein. When the protein of the invention or biologically activeportion thereof is recombinantly produced, optimally culture mediumrepresents less than about 30%, 20%, 10%, 5%, or 1% (by dry weight) ofchemical precursors or non-protein-of-interest chemicals.

The term “fragment” refers to a portion of a polypeptide sequence of theinvention. “Fragments” or “biologically active portions” includepolypeptides comprising a sufficient number of contiguous amino acidresidues to retain the biological activity, i.e., have pesticidalactivity. Fragments of the pesticidal proteins include those that areshorter than the full-length sequences, either due to the use of analternate downstream start site, or due to processing that produces ashorter protein having pesticidal activity. Processing may occur in theorganism the protein is expressed in, or in the pest after ingestion ofthe protein. Examples of fragments of the proteins can be found inTable 1. A biologically active portion of a pesticidal protein can be apolypeptide that is, for example, 10, 25, 50, 100, 150, 200, 250 or moreamino acids in length of any one of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, and/or 458. Suchbiologically active portions can be prepared by recombinant techniquesand evaluated for pesticidal activity. As used here, a fragmentcomprises at least 8 contiguous amino acids of SEQ ID NOs: 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23,24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41,42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59,60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77,78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138,139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152,153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180,181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194,195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208,209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222,223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236,237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250,251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264,265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278,279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320,321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334,335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362,363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376,377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390,391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404,405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418,419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432,433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446,447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, and/or 458.

Bacterial genes, including those encoding the pesticidal proteinsdisclosed herein, quite often possess multiple methionine initiationcodons in proximity to the start of the open reading frame. Often,translation initiation at one or more of these start codons will lead togeneration of a functional protein. These start codons can include ATGcodons. However, bacteria such as Bacillus sp. also recognize the codonGTG as a start codon, and proteins that initiate translation at GTGcodons contain a methionine at the first amino acid. On rare occasions,translation in bacterial systems can initiate at a TTG codon, though inthis event the TTG encodes a methionine. Furthermore, it is not oftendetermined apriori which of these codons are used naturally in thebacterium. Thus, it is understood that use of one of the alternatemethionine codons may also lead to generation of pesticidal proteins.These pesticidal proteins are encompassed in the present invention andmay be used in the methods disclosed herein. It will be understood that,when expressed in plants, it will be necessary to alter the alternatestart codon to ATG for proper translation.

In various embodiments the pesticidal proteins provided herein includeamino acid sequences deduced from the full-length nucleotide sequencesand amino acid sequences that are shorter than the full-length sequencesdue to the use of an alternate downstream start site. Thus, thenucleotide sequence of the invention and/or vectors, host cells, andplants comprising the nucleotide sequence of the invention (and methodsof making and using the nucleotide sequence of the invention) maycomprise a nucleotide sequence encoding an alternate start site.

It is recognized that modifications may be made to the pesticidalpolypeptides provided herein creating variant proteins. Changes designedby man may be introduced through the application of site-directedmutagenesis techniques. Alternatively, native, as yet-unknown or as yetunidentified polynucleotides and/or polypeptides structurally and/orfunctionally-related to the sequences disclosed herein may also beidentified that fall within the scope of the present invention.Conservative amino acid substitutions may be made in nonconservedregions that do not alter the function of the pesticidal proteins.Alternatively, modifications may be made that improve the activity ofthe toxin. Modification of Cry toxins by domain III swapping hasresulted in some cases in hybrid toxins with improved toxicities againstcertain insect species. Thus, domain III swapping could be an effectivestrategy to improve toxicity of Cry toxins or to create novel hybridtoxins with toxicity against pests that show no susceptibility to theparental Cry toxins. Site-directed mutagenesis of domain II loopsequences may result in new toxins with increased insecticidal activity.Domain II loop regions are key binding regions of initial Cry toxinsthat are suitable targets for the mutagenesis and selection of Crytoxins with improved insecticidal properties. Domain I of the Cry toxinmay be modified to introduce protease cleavage sites to improve activityagainst certain pests. Strategies for shuffling the three differentdomains among large numbers of cry genes and high through outputbioassay screening methods may provide novel Cry toxins with improved ornovel toxicities.

As indicated, fragments and variants of the polypeptides disclosedherein will retain pesticidal activity. Pesticidal activity comprisesthe ability of the composition to achieve an observable effectdiminishing the occurrence or an activity of the target pest, includingfor example, bringing about death of at least one pest, or a noticeablereduction in pest growth, feeding, or normal physiological development.Such decreases in numbers, pest growth, feeding or normal developmentcan comprise any statistically significant decrease, including, forexample a decrease of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%,50%, 55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% or greater. The pesticidalactivity against one or more of the various pests provided herein,including, for example, pesticidal activity against Coleoptera, Diptera,Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera,Nematodes, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera,Trichoptera, etc., or any other pest described herein. It is recognizedthat the pesticidal activity may be different or improved relative tothe activity of the native protein, or it may be unchanged, so long aspesticidal activity is retained. Methods for measuring pesticidalactivity are provide elsewhere herein. See also, Czapla and Lang (1990)J. Econ. Entomol. 83:2480-2485; Andrews et al. (1988) Biochem. J.252:199-206; Marrone et al. (1985) J. of Economic Entomology 78:290-293;and U.S. Pat. No. 5,743,477, all of which are herein incorporated byreference in their entirety.

By “variants” is intended polypeptides having an amino acid sequencethat is at least about 60%, about 65%, about 70%, about 75%, about 80%,about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%,about 98% or about 99% identical to the amino acid sequence of any ofSEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71,72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105,106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119,120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133,134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217,218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245,246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259,260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273,274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287,288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301,302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315,316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329,330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343,344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385,386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399,400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427,428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441,442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455,456, 457, and/or 458 and retain pesticidal activity. Note, Table 1provides non-limiting examples of variant polypeptides (andpolynucleotide encoding the same) for each of SEQ ID NOS: 1, 2, 3, 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24,25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42,43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60,61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78,79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111,112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125,126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139,140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153,154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167,168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181,182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195,196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209,210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223,224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237,238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251,252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265,266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279,280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293,294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307,308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321,322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349,350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363,364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377,378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391,392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405,406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419,420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433,434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447,448, 449, 450, 451, 452, 453, 454, 455, 456, 457, and/or 458. Abiologically active variant of a pesticidal polypeptide of the inventionmay differ by as few as about 1-15 amino acid residues, as few as about1-10, such as about 6-10, as few as 5, as few as 4, as few as 3, as fewas 2, or as few as 1 amino acid residue. In specific embodiments, thepolypeptides can comprise an N-terminal or a C-terminal truncation,which can comprise at least a deletion of 10, 15, 20, 25, 30, 35, 40,45, 50 amino acids or more from either the N or C terminal of thepolypeptide.

Table 2 provides protein domains found in SEQ ID NOs: 1-458 based onPFAM data. Both the domain description and the positions within a givenSEQ ID NO are provided in Table 2. In specific embodiments, the activevariant comprising any one of SEQ ID NOs: 1-458 can comprise at least70%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequenceidentity to any one of SEQ ID NOs: 1-458 and further comprises at leastone of the conserved domain set forth in Table 2. For example, in oneembodiment, the active variant will comprise at least 70%, 75%, 76%,77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%,91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQID NO:1, and further comprises the native amino acids at positions23-202.

TABLE 2 Summary of PFAM domains in each of SEQ ID NOs: 1-458 SeqModification PFAM Domain Domain Positions APG ID ID Type DomainDescription Start End APG00929 1 PF06101 DUF946 23 202 APG00954 2PF03318 ETX MTX2 29 252 APG01044 3 no PFAM domains APG01044 modified 4Signal PF03318 ETX MTX2 6 288 (APG01044.1) peptide removed APG01087 5 noPFAM domains APG01087 modified 6 Signal no PFAM (APG01087.1) peptidedomains removed APG01231 7 PF03318 ETX MTX2 65 258 APG01231 modified 8Signal PF03318 ETX MTX2 77 232 (APG01231.1) peptide removed APG01309 9PF01338 Bac thur 10 204 toxin APG01329 10 PF03945 Endotoxin N 98 306PF00555 Endotoxin M 343 533 PF03944 Endotoxin C 543 678 11 PF03945Endotoxin N 75 283 APG01329 modified Alternate PF00555 Endotoxin M 320510 (APG01329.2) start and PF03944 Endotoxin C 520 654 3′ TruncationAPG01329 modified 12 Alternate PF03945 Endotoxin N 75 283 (APG01329.1)start PF00555 Endotoxin M 320 510 PF03944 Endotoxin C 520 655 APG0138713 PF03945 Endotoxin N 81 295 PF00555 Endotoxin M 332 518 PF03944Endotoxin C 528 662 APG01387 modified 14 Alternate PF03945 Endotoxin N76 290 (APG01387.1) start PF00555 Endotoxin M 327 513 PF03944 EndotoxinC 523 657 APG01387 modified 15 Alternate PF03945 Endotoxin N 77 290(APG01387.2) start and PF00555 Endotoxin M 327 513 3′ PF03944 EndotoxinC 523 656 Truncation APG01399 16 PF03945 Endotoxin N 112 316 APG01399modified 17 Signal PF03945 Endotoxin N 74 278 (APG01399.1) peptideremoved APG01459 18 PF03945 Endotoxin N 105 337 PF03944 Endotoxin C 532669 APG01459 modified 19 Signal PF03945 Endotoxin N 75 307 (APG01459.1)peptide PF03944 Endotoxin C 502 639 removed and 3′ Truncation APG0147420 PF07691 PA14 51 179 PF03495 Binary toxB 219 633 APG01474 modified 21Alternate PF07691 PA14 49 177 (APG01474.1) start PF03495 Binary toxB 217631 APG01506 22 PF03318 ETX MTX2 27 250 APG01517 23 no PFAM domainsAPG01517 modified 24 Signal no PFAM (APG01517.1) peptide domains removedAPG01522 25 PF06355 Aegerolysin 5 117 APG01522 modified 26 AlternatePF06355 Aegerolysin 3 115 (APG01522.1) start APG01644 27 PF03945Endotoxin N 99 322 PF03944 Endotoxin C 512 642 PF01473 CW binding 1 694711 PF01473 CW binding 1 723 740 PF01473 CW binding 1 781 800 PF01473 CWbinding 1 802 819 APG01644 modified 28 Signal PF03945 Endotoxin N 69 292(APG01644.1) peptide PF03944 Endotoxin C 482 612 removed and 3′Truncation APG01676 29 PF03318 ETX MTX2 38 324 APG01676 modified 30Signal PF03318 ETX MTX2 9 293 (APG01676.1) peptide removed APG01718 31PF03945 Endotoxin N 99 305 APG01718 modified 32 Alternate PF03945Endotoxin N 78 284 (APG01718.1) start APG01742 33 PF03945 Endotoxin N101 332 PF03944 Endotoxin C 526 661 PF01473 CW binding 1 684 701 PF01473CW binding 1 771 788 PF01473 CW binding 1 800 817 APG01742 modified 34Signal PF03945 Endotoxin N 71 302 (APG01742.1) peptide PF03944 EndotoxinC 496 630 removed and 3′ Truncation APG01772 35 PF03945 Endotoxin N 83282 APG01780 36 PF03945 Endotoxin N 74 326 PF00555 Endotoxin M 337 516PF03944 Endotoxin C 532 671 APG01780 modified 37 Alternate PF03945Endotoxin N 68 279 (APG01780.1) start and PF00555 Endotoxin M 331 510 3′PF03944 Endotoxin C 526 664 Truncation APG01790 38 PF05431 Toxin 10 230425 APG01790 modified 39 Alternate PF05431 Toxin 10 195 390 (APG01790.1)start APG01849 40 no PFAM domains APG01849 modified 41 Alternate no PFAM(APG01849.2) start domains APG01849 modified 42 Signal no PFAM(APG01849.1) peptide domains removed APG01915 43 PF03945 Endotoxin N 84338 PF00555 Endotoxin M 349 528 PF03944 Endotoxin C 544 686 APG01915modified 44 3′ PF03945 Endotoxin N 84 338 (APG01915.1) TruncationPF00555 Endotoxin M 349 528 PF03944 Endotoxin C 544 685 APG01937 45PF06355 Aegerolysin 120 206 APG01996 46 PF03318 ETX MTX2 8 263 APG0216847 no PFAM domains APG02215 PF06101 DUF946 1 233 APG02215 modified 49Alternate PF06101 DUF946 2 214 (APG02215.1) start APG02262 50 no PFAMdomains APG02291 51 PF06355 Aegerolysin 3 115 APG02293 52 no PFAMdomains APG02293 modified 53 Signal no PFAM (APG02293.1) peptide domainsremoved APG02408 54 no PFAM domains APG02408 modified 55 Signal no PFAM(APG02408.2) peptide domains removed APG02408 modified 56 Alternate noPFAM (APG02408.1) start domains APG02445 57 PF05431 Toxin 10 202 396APG02445 modified 58 Signal PF05431 Toxin 10 173 367 (APG02445.1)peptide removed APG02477 59 no PFAM domains APG02477 modified 60Alternate no PFAM (APG02477.1) start domains APG02638 61 PF03318 ETXMTX2 27 252 APG02782 62 no PFAM domains APG02782 modified 63 Signal noPFAM (APG02782.2) peptide domains removed APG02782 modified 64 Alternateno PFAM (APG02782.1) start domains APG02798 65 PF03318 ETX MTX2 38 272APG02798 modified 66 Signal PF03318 ETX MTX2 21 255 (APG02798.1) peptideremoved APG02809 67 no PFAM domains APG02912 68 PF01823 MACPF 132 339APG02912 modified 69 Alternate PF01823 MACPF 121 328 (APG02912.1) startAPG03015 70 PF03318 ETX MTX2 86 311 APG03015 modified 71 Signal PF03318ETX MTX2 54 280 (APG03015.1) peptide removed APG03053 72 PF03318 ETXMTX2 18 287 APG03053 modified 73 Alternate PF03318 ETX MTX2 18 287(APG03053.1) start APG03072 74 no PFAM domains APG03072 modified 75Signal no PFAM (APG03072.1) peptide domains removed APG03080 76 PF01338Bac thur 9 233 toxin APG03227 77 no PFAM domains APG03227 modified 78Signal no PFAM (APG03227.1) peptide domains removed APG03249 79 PF03318ETX MTX2 40 256 APG03249 modified 80 Alternate PF03318 ETX MTX2 36 251(APG03249.1) start APG03299 81 no PFAM domains APG03309 82 PF01338 Bacthur 2 194 toxin APG03309 modified 83 Alternate PF01338 Bac thur 1 194(APG03309.1) start toxin APG03379 84 PF03318 ETX MTX2 21 252 APG03519 85PF03945 Endotoxin N 64 302 PF00555 Endotoxin M 310 496 PF03944 EndotoxinC 506 645 APG03519 modified 86 Alternate PF03945 Endotoxin N 64 302(APG03519.1) start PF00555 Endotoxin M 310 496 PF03944 Endotoxin C 506645 APG03519 modified 87 Alternate PF03945 Endotoxin N 64 302(APG03519.2) start and PF00555 Endotoxin M 310 496 3′ PF03944 EndotoxinC 506 644 Truncation APG03569 88 PF03945 Endotoxin N 66 312 APG03604 89PF03318 ETX MTX2 40 260 APG03699 90 PF03945 Endotoxin N 95 309 APG03699modified 91 Signal PF03945 Endotoxin N 60 275 (APG03699.1) peptideremoved APG03722 92 PF03945 Endotoxin N 182 392 PF00555 Endotoxin M 469693 PF03944 Endotoxin C 703 840 APG03722 modified 93 Alternate PF03945Endotoxin N 101 311 (APG03722.1) start PF00555 Endotoxin M 388 612PF03944 Endotoxin C 622 759 APG03722 modified 94 Alternate PF03945Endotoxin N 101 311 (APG03722.2) start and PF00555 Endotoxin M 388 6123′ PF03944 Endotoxin C 622 758 Truncation APG03726 95 PF01823 MACPF 140354 APG03726 modified 96 Alternate PF01823 MACPF 119 333 (APG03726.1)start APG03732 97 no PFAM domains APG03732 modified 98 Signal no PFAM(APG03732.1) peptide domains removed APG03746 99 PF03318 ETX MTX2 28 288APG03746 modified 100 Signal PF03318 ETX MTX2 9 260 (APG03746.2) peptideremoved APG03746 modified 101 Alternate PF03318 ETX MTX2 26 286(APG03746.1) start APG03786 102 PF03945 Endotoxin N 56 296 PF00555Endotoxin M 304 519 PF03944 Endotoxin C 529 657 APG03786 modified 103Alternate PF03945 Endotoxin N 53 293 (APG03786.1) start PF00555Endotoxin M 301 516 PF03944 Endotoxin C 526 654 APG03848 104 PF03318 ETXMTX2 71 328 APG03848 modified 105 Signal PF03318 ETX MTX2 38 298(APG03848.1) peptide removed APG03901 106 PF03945 Endotoxin N 92 283APG03901 modified 107 Signal PF03945 Endotoxin N 1 176 (APG03901.2)peptide removed APG03901 modified 108 Alternate PF03945 Endotoxin N 13204 (APG03901.1) start APG03947 109 PF03318 ETX MTX2 116 346 APG03947modified 110 Signal PF03318 ETX MTX2 71 301 (APG03947.2) peptide removedAPG03947 modified 111 Alternate PF03318 ETX MTX2 104 334 (APG03947.1)start APG04013 112 no PFAM domains APG04013 modified 113 Alternate noPFAM (APG04013.1) start domains APG04076 114 PF03318 ETX MTX2 28 256APG04076 modified 115 Alternate PF03318 ETX MTX2 21 249 (APG04076.1)start APG04176 116 PF05431 Toxin 10 203 397 APG04176 modified 117 SignalPF05431 Toxin 10 174 368 (APG04176.1) peptide removed APG04332 118PF03945 Endotoxin N 112 325 PF00555 Endotoxin M 333 529 PF03944Endotoxin C 539 676 APG04332 modified 119 Alternate PF03945 Endotoxin N96 309 (APG04332.1) start and PF00555 Endotoxin M 317 513 3′ PF03944Endotoxin C 523 659 Truncation APG04350 120 PF03945 Endotoxin N 76 302PF00555 Endotoxin M 310 520 PF03944 Endotoxin C 530 669 APG04350modified 121 Alternate PF03945 Endotoxin N 70 296 (APG04350.1) start andPF00555 Endotoxin M 304 514 3′ PF03944 Endotoxin C 524 662 TruncationAPG04365 122 PF07691 PA14 77 205 PF03495 Binary toxB 245 658 APG04365modified 123 Alternate PF07691 PA14 74 202 (APG04365.1) start PF03495Binary toxB 242 655 APG04418 124 PF16403 DUF5011 282 355 APG04418modified 125 Signal PF16403 DUF5011 238 311 (APG04418.2) peptide removedAPG04418 modified 126 Alternate PF16403 DUF5011 263 336 (APG04418.1)start APG04431 127 PF03318 ETX MTX2 145 374 APG04431 modified 128 SignalPF03318 ETX MTX2 100 329 (APG04431.1) peptide removed APG04460 129PF03945 Endotoxin N 1 175 PF00555 Endotoxin M 183 400 PF03944 EndotoxinC 410 548 APG04460 modified 130 3′ PF03945 Endotoxin N 1 175(APG04460.1) Truncation PF00555 Endotoxin M 183 400 PF03944 Endotoxin C410 547 APG04460 Split-Cry C- 131 no PFAM terminus (APG01900) domainsAPG04477 132 PF03318 ETX MTX2 69 306 APG04477 modified 133 SignalPF03318 ETX MTX2 37 273 (APG04477.1) peptide removed APG04597 134PF01823 MACPF 311 525 APG04597 modified 135 Alternate PF01823 MACPF 119333 (APG04597.1) start APG04598 136 PF03318 ETX MTX2 39 278 APG04640 137no PFAM domains APG04640 modified 138 Signal no PFAM (APG04640.1)peptide domains removed APG04682 139 PF03945 Endotoxin N 239 340 PF03944Endotoxin C 544 678 APG04682 modified 140 3′ PF03945 Endotoxin N 238 340(APG04682.1) Truncation PF03944 Endotoxin C 544 677 APG04720 141 PF03318ETX MTX2 30 253 APG04720 modified 142 Alternate PF03318 ETX MTX2 27 250(APG04720.1) start APG04725 143 PF01338 Bac thur 16 234 toxin APG04725modified 144 Alternate PF01338 Bac thur 16 234 (APG04725.1) start toxinAPG04804 145 no PFAM domains APG04804 modified 146 Signal no PFAM(APG04804.1) peptide domains removed APG04807 147 PF03318 ETX MTX2 46261 APG04807 modified 148 Signal PF03318 ETX MTX2 15 229 (APG04807.1)peptide removed APG04931 149 PF07691 PA14 48 177 PF03495 Binary toxB 215633 PF09259 Fve 860 961 APG04931 modified 150 Signal PF07691 PA14 21 150(APG04931.1) peptide PF03495 Binary toxB 188 606 removed PF09259 Fve 833934 APG04978 151 PF03318 ETX MTX2 24 248 APG05025 152 PF12495 Vip3A N 12188 APG05025 modified 153 Alternate PF12495 Vip3A N 9 185 (APG05025.1)start APG05034 154 PF03945 Endotoxin N 71 307 PF03944 Endotoxin C 512656 APG05034 modified 155 Alternate PF03945 Endotoxin N 11 247(APG05034.2) start and PF03944 Endotoxin C 452 595 3′ TruncationAPG05034 modified 156 3′ PF03945 Endotoxin N 71 307 (APG05034.1)Truncation PF03944 Endotoxin C 512 655 APG05045 157 PF03318 ETX MTX2 65264 APG05045 modified 158 Alternate PF03318 ETX MTX2 67 260 (APG05045.1)start APG05084 159 no PFAM domains APG05084 modified 160 Signal no PFAM(APG05084.1) peptide domains removed APG05328 161 PF03318 ETX MTX2 23250 APG05370 162 PF03945 Endotoxin N 74 299 PF00555 Endotoxin M 307 522PF03944 Endotoxin C 532 666 APG05370 modified 163 3′ PF03945 Endotoxin N73 299 (APG05370.1) Truncation PF00555 Endotoxin M 307 522 PF03944Endotoxin C 532 665 APG05384 164 no PFAM domains APG05384 modified 165Alternate no PFAM (APG05384.1) start domains APG05384 modified 166Signal no PFAM (APG05384.2) peptide domains removed APG05506 167 PF03318ETX MTX2 76 336 APG05615 168 PF03318 ETX MTX2 67 310 APG05615 modified169 Alternate PF03318 ETX MTX2 43 286 (APG05615.1) start APG05619 170PF05431 Toxin 10 162 357 APG05651 171 no PFAM domains APG05651 modified172 Signal no PFAM (APG05651.1) peptide domains removed APG05653 173 noPFAM domains APG05653 modified 174 Signal no PFAM (APG05653.1) peptidedomains removed APG05658 175 PF03318 ETX MTX2 98 359 APG05658 modified176 Signal PF03318 ETX MTX2 69 330 (APG05658.1) peptide removed APG05715177 PF05431 Toxin 10 202 396 APG05715 modified 178 Signal PF05431 Toxin10 173 367 (APG05715.1) peptide removed APG05804 179 PF03318 ETX MTX2 20273 APG05810 180 PF03945 Endotoxin N 78 295 PF00555 Endotoxin M 303 522PF03944 Endotoxin C 532 665 APG05810 modified 181 3′ PF03945 Endotoxin N78 295 (APG05810.1) Truncation PF00555 Endotoxin M 303 522 PF03944Endotoxin C 532 664 APG05924 182 PF07691 PA14 49 185 PF03495 Binary toxB222 635 APG05924 modified 183 Alternate PF07691 PA14 49 185 (APG05924.1)start PF03495 Binary toxB 222 635 APG05930 184 PF03945 Endotoxin N 68299 PF00555 Endotoxin M 307 525 PF03944 Endotoxin C 536 688 APG05930modified 185 Alternate PF03945 Endotoxin N 68 299 (APG05930.1) start andPF00555 Endotoxin M 307 525 3′ PF03944 Endotoxin C 536 687 TruncationAPG05978 186 PF03945 Endotoxin N 23 193 APG05978 modified 187 AlternatePF03945 Endotoxin N 15 185 (APG05978.1) start APG06051 188 PF14200RicinB lectin 2 42 120 PF05431 Toxin 10 101 296 APG06161 189 no PFAMdomains APG06161 modified 190 Signal no PFAM (APG06161.1) peptidedomains removed APG06176 191 no PFAM domains APG06176 modified 192Alternate no PFAM (APG06176.1) start domains APG06253 193 PF03945Endotoxin N 81 335 PF00555 Endotoxin M 346 527 PF03944 Endotoxin C 542684 APG06253 modified 194 Alternate PF03945 Endotoxin N 68 322(APG06253.1) start and PF00555 Endotoxin M 333 514 3′ PF03944 EndotoxinC 529 670 Truncation APG06364 195 no PFAM domains APG06364 modified 196Signal no PFAM (APG06364.1) peptide domains removed APG06372 197 PF03318ETX MTX2 118 351 APG06372 modified 198 Signal PF03318 ETX MTX2 90 342(APG06372.2) peptide removed APG06372 modified 199 Alternate PF03318 ETXMTX2 118 351 (APG06372.1) start APG06428 200 PF03945 Endotoxin N 25 252PF00555 Endotoxin M 260 465 PF03944 Endotoxin C 475 629 APG06428Split-Cry C- 201 no PFAM terminus (APG02204) domains APG06431 202PF03318 ETX MTX2 73 314 APG06431 modified 203 Signal PF03318 ETX MTX2 20284 (APG06431.1) peptide removed APG06630 204 PF03945 Endotoxin N 64 290PF00555 Endotoxin M 298 493 PF03944 Endotoxin C 505 646 APG06630modified 205 Alternate PF03945 Endotoxin N 62 288 (APG06630.2) start andPF00555 Endotoxin M 296 492 3′ PF03944 Endotoxin C 503 643 TruncationAPG06630 modified 206 Alternate PF03945 Endotoxin N 62 288 (APG06630.1)start PF00555 Endotoxin M 296 491 PF03944 Endotoxin C 503 644 APG06650207 PF03945 Endotoxin N 85 318 PF00555 Endotoxin M 329 489 PF03944Endotoxin C 529 666 APG06650 modified 208 3′ PF03945 Endotoxin N 85 318(APG06650.1) Truncation PF00555 Endotoxin M 329 490 PF03944 Endotoxin C529 665 APG06690 209 PF03318 ETX MTX2 26 246 APG06739 210 PF03945Endotoxin N 74 298 PF00555 Endotoxin M 306 517 PF03944 Endotoxin C 527662 APG06739 modified 211 Alternate PF03945 Endotoxin N 67 291(APG06739.2) start and PF00555 Endotoxin M 299 510 3′ PF03944 EndotoxinC 520 654 Truncation APG06739 modified 212 Alternate PF03945 Endotoxin N67 291 (APG06739.1) start PF00555 Endotoxin M 299 510 PF03944 EndotoxinC 520 655 APG06739 CryBP1 213 PF07029 CryBP1 44 194 (APG01238) APG06768214 PF03318 ETX MTX2 62 276 APG06784 215 no PFAM domains APG06784modified 216 Signal no PFAM (APG06784.1) peptide domains removedAPG06880 217 PF03318 ETX MTX2 32 298 APG06880 modified 218 AlternatePF03318 ETX MTX2 5 269 (APG06880.1) start APG06912 219 no PFAM domainsAPG06912 modified 220 Alternate no PFAM (APG06912.1) start domainsAPG06912 modified 221 Signal no PFAM (APG06912.2) peptide domainsremoved APG06921 222 PF03945 Endotoxin N 78 290 PF00555 Endotoxin M 298513 PF03944 Endotoxin C 523 661 APG06938 223 PF14200 RicinB lectin 43132 2 APG06942 224 PF03945 Endotoxin N 79 303 PF00555 Endotoxin M 311524 PF03944 Endotoxin C 534 674 APG06942 modified 225 Alternate PF03945Endotoxin N 72 297 (APG06942.2) start and PF00555 Endotoxin M 305 518 3′PF03944 Endotoxin C 528 667 Truncation APG06942 modified 226 AlternatePF03945 Endotoxin N 73 297 (APG06942.1) start PF00555 Endotoxin M 305518 PF03944 Endotoxin C 528 668 APG06942 CryBP1 227 PF07029 CryBP1 40190 (APG01239) APG06995 228 PF03945 Endotoxin N 55 255 PF01473 CWbinding 1 346 361 PF01473 CW binding 1 401 414 PF01473 CW binding 1 485498 APG07016 229 PF03945 Endotoxin N 67 288 PF00555 Endotoxin M 353 473PF03944 Endotoxin C 605 746 APG07016 modified 230 Alternate PF03945Endotoxin N 65 286 (APG07016.1) start PF00555 Endotoxin M 351 471PF03944 Endotoxin C 603 744 APG07016 modified 231 Alternate PF03945Endotoxin N 65 286 (APG07016.2) start and PF00555 Endotoxin M 351 474 3′PF03944 Endotoxin C 603 743 Truncation APG07036 232 no PFAM domainsAPG07036 modified 233 Alternate no PFAM (APG07036.1) start domainsAPG07037 234 PF03945 Endotoxin N 70 282 PF00555 Endotoxin M 290 487PF03944 Endotoxin C 497 633 APG07037 modified 235 Alternate PF03945Endotoxin N 70 282 (APG07037.1) start and PF00555 Endotoxin M 290 487 3′PF03944 Endotoxin C 497 632 Truncation APG07058 236 PF03318 ETX MTX2 37245 APG07058 modified 237 Signal PF03318 ETX MTX2 15 217 (APG07058.1)peptide removed APG07100 238 PF03945 Endotoxin N 79 295 PF00555Endotoxin M 303 521 PF03944 Endotoxin C 531 678 APG07100 modified 239 3′PF03945 Endotoxin N 79 295 (APG07100.1) Truncation PF00555 Endotoxin M303 521 PF03944 Endotoxin C 531 677 APG07396 240 PF03318 ETX MTX2 26 274APG07518 241 PF06355 Aegerolysin 85 167 APG07518 modified 242 SignalPF06355 Aegerolysin 56 138 (APG07518.1) peptide removed APG07559 243 noPFAM domains APG07559 modified 244 Alternate no PFAM (APG07559.2) startdomains APG07559 modified 245 Alternate no PFAM (APG07559.1) startdomains APG07655 246 PF03318 ETX MTX2 25 250 APG07672 247 PF03318 ETXMTX2 74 308 APG07672 modified 248 Alternate PF03318 ETX MTX2 67 301(APG07672.1) start APG07672 modified 249 Signal PF03318 ETX MTX2 39 274(APG07672.2) peptide removed APG07731 250 no PFAM domains APG07731modified 251 Signal no PFAM (APG07731.1) peptide domains removedAPG07748 252 PF03318 ETX MTX2 151 384 APG07748 modified 253 SignalPF03318 ETX MTX2 91 329 (APG07748.2) peptide removed APG07748 modified254 Alternate PF03318 ETX MTX2 119 352 (APG07748.1) start APG07783 255PF03318 ETX MTX2 59 320 APG07783 modified 256 Signal PF03318 ETX MTX2 27289 (APG07783.1) peptide removed APG07787 257 PF14200 RicinB lectin 48136 2 APG07790 258 PF07691 PA14 43 170 PF03495 Binary toxB 211 624PF09259 Fve 855 946 APG07790 modified 259 Signal PF07691 PA14 18 145(APG07790.2) peptide PF03495 Binary toxB 186 599 removed PF09259 Fve 830921 APG07790 modified 260 Alternate PF07691 PA14 43 170 (APG07790.1)start PF03495 Binary toxB 211 624 PF09259 Fve 855 946 APG07803 261PF03945 Endotoxin N 89 341 PF00555 Endotoxin M 352 533 PF03944 EndotoxinC 548 690 APG07803 modified 262 Alternate PF03945 Endotoxin N 68 320(APG07803.1) start and PF00555 Endotoxin M 331 512 3′ PF03944 EndotoxinC 527 668 Truncation APG07818 263 PF05431 Toxin 10 207 399 APG07818modified 264 Signal PF05431 Toxin 10 178 370 (APG07818.1) peptideremoved APG07857 265 PF01823 MACPF 117 327 APG07860 266 PF03945Endotoxin N 67 284 PF00555 Endotoxin M 292 495 PF03944 Endotoxin C 505634 APG07860 modified 267 3′ PF03945 Endotoxin N 67 284 (APG07860.1)Truncation PF00555 Endotoxin M 292 495 PF03944 Endotoxin C 505 633APG07866 268 PF03945 Endotoxin N 138 321 PF05588 Botulinum 463 613 HA-17APG07870 269 PF03945 Endotoxin N 97 306 PF00555 Endotoxin M 343 533PF03944 Endotoxin C 543 681 hanne APG07870 modified 270 AlternatePF03945 Endotoxin N 73 283 (APG07870.2) start and PF00555 Endotoxin M320 510 3′ PF03944 Endotoxin C 520 657 Truncation APG07870 modified 271Alternate PF03945 Endotoxin N 74 283 (APG07870.1) start PF00555Endotoxin M 320 510 PF03944 Endotoxin C 520 658 APG07961 272 PF01338 Bacthur 16 240 toxin APG07983 273 PF03318 ETX MTX2 23 262 APG08039 274PF06101 DUF946 27 251 APG08039 modified 275 Alternate PF06101 DUF946 21245 (APG08039.1) start APG08065 276 PF03945 Endotoxin N 116 310 PF00555Endotoxin M 318 541 PF03944 Endotoxin C 551 618 PF03944 Endotoxin C 611670 APG08065 modified 277 3′ PF03945 Endotoxin N 116 310 (APG08065.1)Truncation PF00555 Endotoxin M 318 541 PF03944 Endotoxin C 551 618PF03944 Endotoxin C 611 669 APG08088 278 PF03318 ETX MTX2 30 252APG08088 modified 2.79 Alternate PF03318 ETX MTX2 27 249 (APG08088.1)start APG08108 280 no PFAM domains APG08139 281 PF01338 Bac thur 7 228toxin APG08174 282 PF03945 Endotoxin N 68 293 PF00555 Endotoxin M 301508 PF03944 Endotoxin C 518 656 APG08174 modified 283 Alternate PF03945Endotoxin N 62 288 (APG08174.2) start and PF00555 Endotoxin M 296 503 3′PF03944 Endotoxin C 513 650 Truncation APG08174 modified 284 AlternatePF03945 Endotoxin N 63 288 (APG08174.1) start PF00555 Endotoxin M 296503 PF03944 Endotoxin C 513 651 APG08230 285 PF01338 Bac thur 27 250toxin APG08230 modified 286 Alternate PF01338 Bac thur 16 239(APG08230.1) start toxin APG08278 287 PF03318 ETX MTX2 109 307 APG08278modified 288 Alternate PF03318 ETX MTX2 91 289 (APG08278.1) startAPG08278 modified 289 Signal PF03318 ETX MTX2 59 260 (APG08278.2)peptide removed APG08307 290 PF03318 ETX MTX2 60 281 APG08307 modified291 Signal PF03318 ETX MTX2 28 249 (APG08307.1) peptide removed APG08350292 PF03945 Endotoxin N 70 273 PF00555 Endotoxin M 354 491 PF03944Endotoxin C 570 703 APG08350 modified 293 3′ PF03945 Endotoxin N 70 273(APG08350.1) Truncation PF00555 Endotoxin M 354 495 PF03944 Endotoxin C570 703 APG08396 294 PF03945 Endotoxin N 43 267 PF00555 Endotoxin M 275466 PF03944 Endotoxin C 476 613 APG08396 modified 295 Alternate PF03945Endotoxin N 34 258 (APG08396.1) start PF00555 Endotoxin M 266 457PF03944 Endotoxin C 467 604 APG08396 modified 296 Alternate PF03945Endotoxin N 34 258 (APG08396.2) start and PF00555 Endotoxin M 266 457 3′PF03944 Endotoxin C 467 603 Truncation APG08461 297 no PFAM domainsAPG08525 298 no PFAM domains APG08525 modified 299 Alternate no PFAM(APG08525.1) start domains APG08589 300 PF03318 ETX MTX2 27 249 APG08631301 PF01338 Bac thur 30 250 toxin APG08631 modified 302 AlternatePF01338 Bac thur 14 234 (APG08631.1) start toxin APG08657 303 PF03945Endotoxin N 89 295 PF03944 Endotoxin C 525 664 APG08657 modified 304 3′PF03945 Endotoxin N 88 295 (APG08657.1) Truncation PF00555 Endotoxin M303 515 PF03944 Endotoxin C 525 663 APG08665 305 PF03945 Endotoxin N 118348 APG08665 modified 306 Signal PF03945 Endotoxin N 80 310 (APG08665.2)peptide removed and 3′ Truncation APG08665 modified 307 Signal PF03945Endotoxin N 80 310 (APG08665.1) peptide removed APG08693 308 no PFAMdomains APG08693 modified 309 Signal no PFAM (APG08693.1) peptidedomains removed APG08817 310 no PFAM domains APG08817 modified 311Alternate no PFAM (APG08817.1) start domains APG08817 modified 312Signal no PFAM (APG08817.2) peptide domains removed APG08898 313 no PFAMdomains APG08898 modified 314 Alternate no PFAM (APG08898.1) startdomains APG08919 315 no PFAM domains APG08919 modified 316 Alternate noPFAM (APG08919.1) start domains APG08931 317 PF03945 Endotoxin N 66 304PF03944 Endotoxin C 515 670 APG08931 modified 318 3′ PF03945 Endotoxin N66 304 (APG08931.1) Truncation PF03944 Endotoxin C 515 669 APG09084 319PF03318 ETX MTX2 19 273 APG09232 320 no PFAM domains APG09232 modified321 Signal no PFAM (APG09232.1) peptide domains removed APG09234 322PF03495 Binary toxB 94 516 PF03318 ETX MTX2 156 285 APG09234 CryBP1 323PF07029 CryBP1 43 193 (APG08851) APG09296 324 PF12495 Vip3A N 16 191APG09296 modified 325 Alternate PF12495 Vip3A N 12 187 (APG09296.1)start APG09413 326 no PFAM domains APG09484 327 no PFAM domains APG09484modified 328 Signal no PFAM (APG09484.1) peptide domains removedAPG09543 329 PF03945 Endotoxin N 94 292 APG09543 modified 330 SignalPF03945 Endotoxin N 56 254 (APG09543.1) peptide removed APG09545 331PF07691 PA14 64 188 PF03495 Binary toxB 226 643 APG09545 modified 332Alternate PF07691 PA14 45 169 (APG09545.1) start PF03495 Binary toxB 207624 APG09573 333 PF03945 Endotoxin N 90 315 PF00555 Endotoxin M 323 530PF03944 Endotoxin C 540 678 APG09573 modified 334 Alternate PF03945Endotoxin N 62 288 (APG09573.2) start and PF00555 Endotoxin M 296 503 3′PF03944 Endotoxin C 513 650 Truncation APG09573 modified 335 AlternatePF03945 Endotoxin N 63 288 (APG09573.1) start PF00555 Endotoxin M 296503 PF03944 Endotoxin C 513 651 APG09589 336 PF06355 Aegerolysin 66 178APG09630 337 PF03945 Endotoxin N 176 377 APG09630 modified 338 AlternatePF03945 Endotoxin N 139 340 (APG09630.1) start APG09682 339 PF03318 ETXMTX2 24 251 APG09864 340 PF03318 ETX MTX2 39 301 APG09864 modified 341Signal PF03318 ETX MTX2 9 264 (APG09864.1) peptide removed APG09877 342PF03945 Endotoxin N 76 312 PF00555 Endotoxin M 320 554 PF03944 EndotoxinC 564 692 APG09936 343 PF03945 Endotoxin N 15 184 APG09936 modified 344Alternate PF03945 Endotoxin N 15 184 (APG09936.1) start APG09947 345PF03945 Endotoxin N 103 330 APG09947 modified 346 Signal PF03945Endotoxin N 62 289 (APG09947.1) peptide removed APG09984 347 PF07691PA14 15 141 PF03495 Binary toxB 186 598 PF09259 Fve 841 929 APG00905.0348 PF05431 Insecticidal 207 395 Crystal Toxin, P42 APG00905.1 349Signal PF05431 Insecticidal 178 366 peptide Crystal Toxin, removed P42APG02585.0 350 PF07691 PA14 domain 52 182 PF03495 Clostridial 223 629binary toxin B/anthrax toxin PA APG02585.1 351 Alternate PF07691 PA14domain 50 180 start PF03495 Clostridial 221 627 binary toxin B/anthraxtoxin PA APG03000.0 352 PF03945 delta 41 272 endotoxin, N- terminaldomain PF00555 delta 280 470 endotoxin PF03944 delta 480 616 endotoxinAPG03995.0 353 PF03318 Clostridium 142 250 epsilon toxin ETX/Bacillusmosquitocidal toxin MTX2 APG03995.1 354 Signal PF03318 Clostridium 93201 peptide epsilon toxin removed ETX/Bacillus mosquitocidal toxin MTX2APG04196.0 355 no PFAM domains APG04196.1 356 Signal no PFAM peptidedomains removed APG05497.0 357 PF03318 Clostridium 84 285 epsilon toxinETX/Bacillus mosquitocidal toxin MTX2 APG05497.1 358 Signal PF03318Clostridium 56 257 peptide epsilon toxin removed ETX/Bacillusmosquitocidal toxin MTX2 APG06291.0 359 PF03318 Clostridium 123 252epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG06291.1 360Signal PF03318 Clostridium 94 228 peptide epsilon toxin removedETX/Bacillus mosquitocidal toxin MTX2 APG06371.0 361 PF03318 Clostridium94 297 epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG06371.1362 Signal PF03318 Clostridium 61 264 peptide epsilon toxin removedETX/Bacillus mosquitocidal toxin MTX2 APG07577.0 363 PF07691 PA14 domain168 PF03495 Clostridial 211 623 binary toxin B/anthrax toxin PA PF09259Fungal 866 935 immunomodulatory protein Fve APG07577.1 364 SignalPF07691 PA14 domain 20 143 peptide removed PF03495 Clostridial 186 598binary toxin B/anthrax toxin PA PF09259 Fungal 841 910 immunomodulatoryprotein Fve APG07648.0 365 no PFAM domains APG07648.1 366 Signal no PFAMpeptide domains removed APG09231.0 367 PF03318 Clostridium 44 242epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG00923.0 368PF03318 Clostridium 139 343 epsilon toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG00923.1 369 Alternate PF03318 Clostridium 139 343 startepsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG00923.2 370Signal PF03318 Clostridium 113 315 peptide epsilon toxin removedETX/Bacillus mosquitocidal toxin MTX2 APG00941.0 371 PF03945 delta 81301 endotoxin, N- terminal domain PF00555 delta 307 523 endotoxinPF03944 delta 534 686 endotoxin APG00941.1 372 3′ PF03945 delta 81 301Truncation endotoxin, N- terminal domain PF00555 delta 307 523 endotoxinPF03944 delta 534 686 endotoxin APG01202.0 373 no PFAM domains APG01202CryBP1 374 PF07029 CryBP1 3 48 (APG04093.0) protein APG01809.0 375PF14200 Ricin-type 4 78 beta-trefoil lectin domain- like PF05431Insecticidal 86 278 Crystal Toxin, P42 APG01809,1 376 Alternate PF14200Ricin-type 4 78 start beta-trefoil lectin domain- like PF05431Insecticidal 86 278 Crystal Toxin, P42 APG02194.0 377 PF03318Clostridium 139 343 epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2APG02194.1 378 Alternate PF03318 Clostridium 139 343 start epsilon toxinETX/Bacillus mosquitocidal toxin MTX2 APG02194.2 379 Signal PF03318Clostridium 111 315 peptide epsilon toxin removed ETX/Bacillusmosquitocidal toxin MTX2 APG02362.0 380 PF03318 Clostridium 141 342epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG02362.1 381Alternate PF03318 Clostridium 141 342 start epsilon toxin ETX/Bacillusmosquitocidal toxin MTX2 APG02362.2 382 Signal PF03318 Clostridium 113314 peptide epsilon toxin removed ETX/Bacillus mosquitocidal toxin MTX2APG02421.0 383 PF03318 Clostridium 150 351 epsilon toxin ETX/Bacillusmosquitocidal toxin MTX2 APG02421.1 384 Alternate PF03318 Clostridium142 343 start epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2APG02421,2 385 Signal PF03318 Clostridium 114 315 peptide epsilon toxinremoved ETX/Bacillus mosquitocidal toxin MTX2 APG02686.0 386 PF03318Clostridium 150 351 epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2APG02686.1 387 Alternate PF03318 Clostridium 142 343 start epsilon toxinETX/Bacillus mosquitocidal toxin MTX2 APG02686.2 388 Signal PF03318Clostridium 114 315 peptide epsilon toxin removed ETX/Bacillusmosquitocidal toxin MTX2 APG03297.0 389 PF03318 Clostridium 96 297epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG03297.1 390Alternate PF03318 Clostridium 82 283 start epsilon toxin ETX/Bacillusmosquitocidal toxin MTX2 APG03297.2 391 Signal PF03318 Clostridium 56257 peptide epsilon toxin removed ETX/Bacillus mosquitocidal toxin MTX2APG03337.0 392 PF03318 Clostridium 139 343 epsilon toxin ETX/Bacillusmosquitocidal toxin MTX2 APG03337.1 393 Signal PF03318 Clostridium 111315 peptide epsilon toxin removed ETX/Bacillus mosquitocidal toxin MTX2APG03352.0 394 PF03318 Clostridium 141 252 epsilon toxin ETX/Bacillusmosquitocidal toxin MTX2 APG03352.1 395 Signal PF03318 Clostridium 94205 peptide epsilon toxin removed ETX/Bacillus mosquitocidal toxin MTX2APG03422.0 396 PF03318 Clostridium 174 378 epsilon toxin ETX/Bacillusmosquitocidal toxin MTX2 APG03438.0 397 PF03945 delta 21 170 endotoxin,N- terminal domain APG03438.1 398 Alternate PF03945 delta 21 170 startendotoxin, N- terminal domain APG03891.0 399 PF03318 Clostridium 59 248epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG04251.0 400 noPFAM domains APG04251.1 401 Signal no PFAM peptide domains removedAPG04458.0 402 PF03945 delta 22 170 endotoxin, N- terminal domainAPG04458.1 403 Alternate PF03945 delta 22 170 start endotoxin, N-terminal domain APG04650.0 404 PF03318 Clostridium 139 343 epsilon toxinETX/Bacillus mosquitocidal toxin MTX2 APG04650.1 405 Alternate PF03318Clostridium 139 343 start epsilon toxin ETX/Bacillus mosquitocidal toxinMTX2 APG04650.2 406 Signal PF03318 Clostridium 110 315 epsilon toxinpeptide ETX/Bacillus removed mosquitocidal toxin MTX2 APG04806.0 407 noPFAM domains APG04806.1 408 Signal no PFAM peptide domains removedAPG05226.0 409 PF01117 Aerolysin 140 269 toxin APG05226.1 410 SignalPF01117 Aerolysin 114 243 peptide toxin removed APG05337.0 411 no PFAMdomains APG05337.1 412 Signal no PFAM peptide domains removed APG06330.0413 PF05431 Insecticidal 237 428 Crystal Toxin, P42 APG06330.1 414Alternate PF05431 Insecticidal 195 386 start Crystal Toxin, P42APG06330.2 415 Alternate PF05431 Insecticidal 164 355 start CrystalToxin, P42 APG06841.0 416 no PFAM domains APG06841.1 417 Signal no PFAMpeptide domains removed APG07160.0 418 no PFAM domains APG07160.1 419Alternate no PFAM start domains APG07160.2 420 Signal no PFAM peptidedomains removed APG07724.0 421 PF03945 delta 69 328 endotoxin, N-terminal domain PF00555 delta 337 515 endotoxin PF03944 delta 532 670endotoxin APG07724.1 422 Alternate PF03945 delta 63 322 start and 3′endotoxin, N- Truncation terminal domain PF00555 delta 331 509 endotoxinPF03944 delta 526 664 endotoxin APG07936.0 423 no PFAM domainsAPG07936.1 424 Signal no PFAM peptide domains removed APG08381.0 425PF03318 Clostridium 149 350 epsilon toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG08381.1 426 Alternate PF03318 Clostridium 109 310 startepsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG09755.0 427PF03318 Clostridium 124 236 epsilon toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG09755.1 428 Signal PF03318 Clostridium 93 205 peptideepsilon toxin removed ETX/Bacillus mosquitocidal toxin MTX2 APG09824.0429 PF03945 delta 21 170 endotoxin, N- terminal domain APG09824.1 430Alternate PF03945 delta 21 170 start endotoxin, N- terminal domainAPG01515.0 431 PF03945 delta 71 320 endotoxin, N- terminal domainPF00555 delta 331 509 endotoxin PF03944 delta 526 664 endotoxinAPG01515.1 432 3′ PF03945 delta 71 260 Truncation endotoxin, N- terminaldomain PF00555 delta 331 509 endotoxin PF03944 delta 526 664 endotoxinAPG01515 CryBP1 433 no PFAM (APG08269.0) domains APG01949.0 434 no PFAMdomains APG02436.0 435 PF03318 Clostridium 142 247 epsilon toxinETX/Bacillus mosquitocidal toxin MTX2 APG02436.1 436 Signal PF03318Clostridium 93 198 peptide epsilon toxin removed ETX/Bacillusmosquitocidal toxin MTX2 APG02554.0 437 PF03318 Clostridium 46 248epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG02886.0 438PF01338 Bacillus 20 232 thuringiensis toxin APG02886.1 439 AlternatePF01338 Bacillus 20 232 start thuringiensis toxin APG03861.0 440 PF03318Clostridium 65 314 epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2APG03861.1 441 Alternate PF03318 Clostridium 62 311 epsilon toxin startETX/Bacillus mosquitocidal toxin MTX2 APG03861.2 442 Signal PF03318Clostridium 34 283 peptide epsilon toxin removed ETX/Bacillusmosquitocidal toxin MTX2 APG04374.0 443 PF03945 delta 107 322 endotoxin,N- terminal domain PF03944 delta 512 641 endotoxin PF01473 Putative cell694 710 wall binding repeat PF01473 Putative cell 781 800 wall bindingrepeat PF01473 Putative cell 803 818 wall binding repeat APG04374.1 4443′ PF03945 delta 107 322 Truncation endotoxin, N- terminal domainPF03944 delta 512 641 endotoxin APG04374.2 445 Signal PF03945 delta 77292 peptide endotoxin, N- removed terminal domain PF03944 delta 482 611endotoxin PF01473 Putative cell 664 680 wall binding repeat PF01473Putative cell 694 708 wall binding repeat PF01473 Putative cell 751 770wall binding repeat PF01473 Putative cell 773 788 wall binding repeatAPG04374.3 446 Signal PF03945 delta 77 292 peptide endotoxin, N- removedterminal and 3′ domain Truncation PF03944 delta 482 611 endotoxinAPG04547.0 447 no PFAM domains APG04547.1 448 Signal no PFAM peptidedomains removed APG05852.0 449 no PFAM domains APG05852.1 450 Signal noPFAM peptide domains removed APG06492.0 451 PF03318 Clostridium 24 271epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2 APG06587.0 452PF03945 delta 113 292 endotoxin, N- terminal domain APG06587.1 453Signal PF03945 delta 75 254 peptide endotoxin, N- removed terminaldomain APG07911.0 454 PF14200 Ricin-type 31 98 beta-trefoil lectindomain- like PF05431 Insecticidal 236 396 Crystal Toxin, P42 APG07911.1455 Signal PF14200 Ricin-type 4 68 peptide beta-trefoil removed lectindomain- like PF05431 Insecticidal 207 367 Crystal Toxin, P42 APG08051.0456 no PFAM domains APG08051.1 457 Alternate no PFAM start domainsAPG08051.2 458 Signal no PFAM peptide domains removed

Recombinant or synthetic nucleic acids encoding the pesticidalpolypeptides disclosed herein are also provided. Of particular interestare nucleic acid sequences that have been designed for expression in aplant of interest. That is, the nucleic acid sequence can be optimizedfor increased expression in a host plant. A pesticidal protein of theinvention can be back-translated to produce a nucleic acid comprisingcodons optimized for expression in a particular host, for example, acrop plant. In another embodiment, the polynucleotides encoding thepolypeptides provided herein may be optimized for increased expressionin the transformed plant. That is, the polynucleotides can besynthesized using plant-preferred codons for improved expression. See,for example, Campbell and Gowri (1990) Plant Physiol. 92:1-11 for adiscussion of host-preferred codon usage. Methods are available in theart for synthesizing plant-preferred genes. See, for example, U.S. Pat.Nos. 5,380,831, and 5,436,391, and Murray et al. (1989) Nucleic AcidsRes. 17:477-498, herein incorporated by reference. Expression of such acoding sequence by the transformed plant (e.g., dicot or monocot) willresult in the production of a pesticidal polypeptide and conferincreased resistance in the plant to a pest. Recombinant and syntheticnucleic acid molecules encoding the pesticidal proteins of the inventiondo not include the naturally occurring bacterial sequence encoding theprotein.

A “recombinant polynucleotide” or “recombinant nucleic acid” comprises acombination of two or more chemically linked nucleic acid segments whichare not found directly joined in nature. By “directly joined” isintended the two nucleic acid segments are immediately adjacent andjoined to one another by a chemical linkage. In specific embodiments,the recombinant polynucleotide comprises a polynucleotide of interest ora variant or fragment thereof such that an additional chemically linkednucleic acid segment is located either 5′, 3′ or internal to thepolynucleotide of interest. Alternatively, the chemically-linked nucleicacid segment of the recombinant polynucleotide can be formed by deletionof a sequence. The additional chemically linked nucleic acid segment orthe sequence deleted to join the linked nucleic acid segments can be ofany length, including for example, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20or greater nucleotides. Various methods for making such recombinantpolynucleotides include chemical synthesis or by the manipulation ofisolated segments of polynucleotides by genetic engineering techniques.In specific embodiments, the recombinant polynucleotide can comprise arecombinant DNA sequence or a recombinant RNA sequence. A “fragment of arecombinant polynucleotide or nucleic acid” comprises at least one of acombination of two or more chemically linked amino acid segments whichare not found directly joined in nature.

Fragments of a polynucleotide (RNA or DNA) may encode protein fragmentsthat retain activity. In specific embodiments, a fragment of arecombinant polynucleotide or a recombinant polynucleotide constructcomprises at least one junction of the two or more chemically linked oroperably linked nucleic acid segments which are not found directlyjoined in nature. A fragment of a polynucleotide that encodes abiologically active portion of a polypeptide that retains pesticidalactivity will encode at least 25, 30, 40, 50, 60, 70, 75, 80, 90, 100,110, 120, 125, 130, 140, 150, 160, 170, 175, 180, contiguous aminoacids, or up to the total number of amino acids present in a full-lengthpolypeptide as set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10,11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46,47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64,65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82,83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, and/or 458. In specific embodiments,such polypeptide fragments are active fragment, and in still otherembodiments, the polypeptide fragment comprises a recombinantpolypeptide fragment. As used herein, a fragment of a recombinantpolypeptide comprises at least one of a combination of two or morechemically linked amino acid segments which are not found directlyjoined in nature.

By “Variants” is intended to mean substantially similar sequences. Forpolynucleotides, a variant comprises a deletion and/or addition of oneor more nucleotides at one or more internal sites within the nativepolynucleotide and/or a substitution of one or more nucleotides at oneor more sites in the native polynucleotide. As used herein, a “native”polynucleotide or polypeptide comprises a naturally occurring nucleotidesequence or amino acid sequence, respectively.

Variants of a particular polynucleotide of the invention (i.e., thereference polynucleotide) can also be evaluated by comparison of thepercent sequence identity between the polypeptide encoded by a variantpolynucleotide and the polypeptide encoded by the referencepolynucleotide. Thus, for example, an isolated polynucleotide thatencodes a polypeptide with a given percent sequence identity to thepolypeptide of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68,69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103,104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117,118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131,132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145,146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159,160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173,174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187,188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201,202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215,216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229,230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243,244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271,272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285,286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299,300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313,314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327,328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341,342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355,356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369,370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383,384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397,398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411,412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425,426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439,440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453,454, 455, 456, 457, and/or 458 are disclosed. Percent sequence identitybetween any two polypeptides can be calculated using sequence alignmentprograms and parameters described elsewhere herein. Where any given pairof polynucleotides of the invention is evaluated by comparison of thepercent sequence identity shared by the two polypeptides they encode,the percent sequence identity between the two encoded polypeptides is atleast about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or moresequence identity to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129,130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157,158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171,172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185,186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199,200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213,214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255,256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269,270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297,298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311,312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339,340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353,354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367,368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381,382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395,396, 397, 398, 399, 400, 401,402, 403, 404, 405, 406, 407, 408, 409,410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437,438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451,452, 453, 454, 455, 456, 457, and/or 458. In other embodiments, thevariant of the polynucleotide provided herein differs from the nativesequence by at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more nucleotides.

Variant polynucleotide and proteins also encompass sequences andproteins derived from a mutagenic and recombinogenic procedure such asDNA shuffling. With such a procedure, one or more different pesticidalprotein disclosed herein (SEQ ID NO: 1-458) is manipulated to create anew pesticidal protein possessing the desired properties. In thismanner, libraries of recombinant polynucleotides are generated from apopulation of related sequence polynucleotides comprising sequenceregions that have substantial sequence identity and can be homologouslyrecombined in vitro or in vivo. For example, using this approach,sequence motifs encoding a domain of interest may be shuffled betweenthe pesticidal sequences provided herein and other known pesticidalgenes to obtain a new gene coding for a protein with an improvedproperty of interest, such as an increased K_(m) in the case of anenzyme. Strategies for such DNA shuffling are known in the art. See, forexample, Stemmer (1994) Proc. Natl. Acad. Sci. USA 91:10747-10751;Stemmer (1994) Nature 370:389-391; Crameri et al. (1997) Nature Biotech.15:436-438; Moore et al. (1997) J. Mol. Biol. 272:336-347; Zhang et al.(1997) Proc. Natl. Acad. Sci. USA 94:4504-4509; Crameri et al. (1998)Nature 391:288-291; and U.S. Pat. Nos. 5,605,793 and 5,837,458. A“shuffled” nucleic acid is a nucleic acid produced by a shufflingprocedure such as any shuffling procedure set forth herein. Shufflednucleic acids are produced by recombining (physically or virtually) twoor more nucleic acids (or character strings), for example in anartificial, and optionally recursive, fashion. Generally, one or morescreening steps are used in shuffling processes to identify nucleicacids of interest; this screening step can be performed before or afterany recombination step. In some (but not all) shuffling embodiments, itis desirable to perform multiple rounds of recombination prior toselection to increase the diversity of the pool to be screened. Theoverall process of recombination and selection are optionally repeatedrecursively. Depending on context, shuffling can refer to an overallprocess of recombination and selection, or, alternately, can simplyrefer to the recombinational portions of the overall process.

In one embodiments, a method of obtaining a polynucleotide that encodesan improved polypeptide comprising pesticidal activity is provided,wherein the improved polypeptide has at least one improved property overany one of SEQ ID NOS: 1-458. Such methods can comprises (a) recombininga plurality of parental polynucleotides to produce a library ofrecombinant polynucleotides encoding recombinant pesticidalpolypeptides; (b) screening the library to identify a recombinantpolynucleotide that encodes an improved recombinant pesticidalpolypeptide that has an enhanced property improved over the parentalpolynucleotide; (c) recovering the recombinant polynucleotide thatencodes the improved recombinant pesticidal polypeptide identified in(b); and, (d) repeating steps (a), (b) and (c) using the recombinantpolynucleotide recovered in step (c) as one of the plurality of parentalpolynucleotides in repeated step (a).

iii. Sequence Comparisons

As used herein, the term “identity” or “percent identity” when used withrespect to a particular pair of aligned amino acid sequences, refers tothe percent amino acid sequence identity that is obtained by countingthe number of identical matches in the alignment and dividing suchnumber of identical matches by the length of the aligned sequences. Asused herein, the term “similarity” or “percent similarity” when usedwith respect to a particular pair of aligned amino acid sequences,refers to the sum of the scores that are obtained from a scoring matrixfor each amino acid pair in the alignment divided by the length of thealigned sequences.

Unless otherwise stated, identity and similarity will be calculated bythe Needleman-Wunsch global alignment and scoring algorithms (Needlemanand Wunsch (1970) J. Mol. Biol. 48(3):443-453) as implemented by the“needle” program, distributed as part of the EMBOSS software package(Rice, P. Longden, I. and Bleasby, A., EMBOSS: The European MolecularBiology Open Software Suite, 2000, Trends in Genetics 16, (6) pp276-277, versions 6.3.1 available from EMBnet atembnet.org/resource/emboss and emboss.sourceforge.net, among othersources) using default gap penalties and scoring matrices (EBLOSUM62 forprotein and EDNAFULL for DNA). Equivalent programs may also be used. By“equivalent program” is intended any sequence comparison program that,for any two sequences in question, generates an alignment havingidentical nucleotide residue matches and an identical percent sequenceidentity when compared to the corresponding alignment generated byneedle from EMBOSS version 6.3.1.

Additional mathematical algorithms are known in the art and can beutilized for the comparison of two sequences. See, for example, thealgorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA87:2264, modified as in Karlin and Altschul (1993) Proc. Natl. Acad.Sci. USA 90:5873-5877. Such an algorithm is incorporated into the BLASTprograms of Altschul et al. (1990) J. Mol. Biol. 215:403. BLASTnucleotide searches can be performed with the BLASTN program (nucleotidequery searched against nucleotide sequences) to obtain nucleotidesequences homologous to pesticidal-like nucleic acid molecules of theinvention, or with the BLASTX program (translated nucleotide querysearched against protein sequences) to obtain protein sequenceshomologous to pesticidal nucleic acid molecules of the invention. BLASTprotein searches can be performed with the BLASTP program (protein querysearched against protein sequences) to obtain amino acid sequenceshomologous to pesticidal protein molecules of the invention, or with theTBLASTN program (protein query searched against translated nucleotidesequences) to obtain nucleotide sequences homologous to pesticidalprotein molecules of the invention. To obtain gapped alignments forcomparison purposes, Gapped BLAST (in BLAST 2.0) can be utilized asdescribed in Altschul et al. (1997) Nucleic Acids Res. 25:3389.Alternatively, PSI-Blast can be used to perform an iterated search thatdetects distant relationships between molecules. See Altschul et al.(1997) supra. When utilizing BLAST, Gapped BLAST, and PSI-Blastprograms, the default parameters of the respective programs (e.g.,BLASTX and BLASTN) can be used. Alignment may also be performed manuallyby inspection.

Two sequences are “optimally aligned” when they are aligned forsimilarity scoring using a defined amino acid substitution matrix (e.g.,BLOSUM62), gap existence penalty and gap extension penalty so as toarrive at the highest score possible for that pair of sequences. Aminoacid substitution matrices and their use in quantifying the similaritybetween two sequences are well-known in the art and described, e.g., inDayhoff et al. (1978) “A model of evolutionary change in proteins.” In“Atlas of Protein Sequence and Structure,” Vol. 5, Suppl. 3 (ed. M. O.Dayhoff), pp. 345-352. Natl. Biomed. Res. Found., Washington, D.C. andHenikoff et al. (1992) Proc. Natl. Acad. Sci. USA 89:10915-10919. TheBLOSUM62 matrix is often used as a default scoring substitution matrixin sequence alignment protocols. The gap existence penalty is imposedfor the introduction of a single amino acid gap in one of the alignedsequences, and the gap extension penalty is imposed for each additionalempty amino acid position inserted into an already opened gap. Thealignment is defined by the amino acids positions of each sequence atwhich the alignment begins and ends, and optionally by the insertion ofa gap or multiple gaps in one or both sequences, so as to arrive at thehighest possible score. While optimal alignment and scoring can beaccomplished manually, the process is facilitated by the use of acomputer-implemented alignment algorithm, e.g., gapped BLAST 2.0,described in Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402, andmade available to the public at the National Center for BiotechnologyInformation Website (found on the world wide web at ncbi.nih.gov).Optimal alignments, including multiple alignments, can be preparedusing, e.g., PSI-BLAST, available on the world wide web atncbi.nlm.nih.gov and described by Altschul et al. (1997) Nucleic AcidsRes. 25:3389-3402.

With respect to an amino acid sequence that is optimally aligned with areference sequence, an amino acid residue “corresponds to” the positionin the reference sequence with which the residue is paired in thealignment. The “position” is denoted by a number that sequentiallyidentifies each amino acid in the reference sequence based on itsposition relative to the N-terminus. For example, in SEQ ID NO: 1position 1 is M, position 2 is S, position 3 is I, etc. When a testsequence is optimally aligned with SEQ ID NO: 1, a residue in the testsequence that aligns with the I at position 3 is said to “correspond toposition 3” of SEQ ID NO: 1. Owing to deletions, insertion, truncations,fusions, etc., that must be taken into account when determining anoptimal alignment, in general the amino acid residue number in a testsequence as determined by simply counting from the N-terminal will notnecessarily be the same as the number of its corresponding position inthe reference sequence. For example, in a case where there is a deletionin an aligned test sequence, there will be no amino acid thatcorresponds to a position in the reference sequence at the site ofdeletion. Where there is an insertion in an aligned reference sequence,that insertion will not correspond to any amino acid position in thereference sequence. In the case of truncations or fusions there can bestretches of amino acids in either the reference or aligned sequencethat do not correspond to any amino acid in the corresponding sequence.

iv. Antibodies

Antibodies to the polypeptides of the present invention, or to variantsor fragments thereof, are also encompassed. Methods for producingantibodies are well known in the art (see, for example, Harlow and Lane(1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory,Cold Spring Harbor, N.Y.; and U.S. Pat. No. 4,196,265). These antibodiescan be used in kits for the detection and isolation of toxinpolypeptides. Thus, this disclosure provides kits comprising antibodiesthat specifically bind to the polypeptides described herein, including,for example, polypeptides having the sequence of SEQ ID NOs: 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23,24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41,42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59,60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77,78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138,139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152,153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180,181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194,195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208,209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222,223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236,237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250,251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264,265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278,279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320,321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334,335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362,363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376,377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390,391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404,405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418,419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432,433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446,447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, and/or 458.

II. Pests

The compositions and methods provided herein are useful against avariety of pests. “Pests” includes but is not limited to, insects,fungi, bacteria, nematodes, acarids, protozoan pathogens,animal-parasitic liver flukes, and the like. Pests of particularinterest are insect pests, particularly insect pests that causesignificant damage to agricultural plants. Insect pests include insectsselected from the orders Coleoptera, Diptera, Hymenoptera, Lepidoptera,Mallophaga, Homoptera, Hemiptera, Orthroptera, Thysanoptera, Dermaptera,Isoptera, Anoplura, Siphonaptera, Trichoptera, or nematodes. Innon-limiting embodiments, the insect pest comprises Western cornrootworm, Diabrotica virgifera virgifera; Fall armyworm, Spodopterafrugiperda; Colorado potato beetle, Leptinotarsa decemlineata; Cornearworm, Helicoverpa zea (in North America same species attacks cottonand called cotton bollworm); European corn borer, Ostrinia nubilalis;Black cutworm, Agrotis ipsilon; Diamondback moth, Plutella xylostella;Velvetbean caterpillar, Anticarsia gemmatalis; Southwestern corn borer,Diatraea grandiosella; Cotton bollworm, Helicoverpa armigera (foundother than USA in rest of the world); Southern green stinkbug, Nezaraviridula; Green stinkbug, Chinavia halaris; Brown marmorated stinkbug,Halyomorpha halys; and Brown stinbug, Euschistus servus, Euschistusheros (Neotropical brown stink bug OR soy stink bug); Piezodorusguildinii (red-banded stink bug); Dichelops melacanthus (no common name)and/or Dichelops furcatus (no common name); an aphid, such as a soybeanaphid. In other embodiments, the pest comprises a nematode including,but not limited to, Meloidogyne hapla (Northern root-knot nematode);Meloidogyne enterolobii, Meloidogyne arenaria (peanut root-knotnematode); and Meloidogyne javanica.

The term “insect pests” as used herein refers to insects and othersimilar pests such as, for example, those of the order Acari including,but not limited to, mites and ticks. Insect pests of the presentinvention include, but are not limited to, insects of the orderLepidoptera, e.g. Achoroia grisella, Acleris gloverana, Acleris variana,Adoxophyes orana, Agrolis ipsilon, Alabama argillacea, Alsophilapometaria, Amyelois transitella, Anagasta kuehniella, Anarsialineatella, Anisota senatoria, Antheraea pernyi, Anticarsia gemmatalis,Archips sp., Argyrotaenia sp., Athetis mindara, Bombyx mori, Bucculatrixthurberiella, Cadra cautella, Choristoneura sp., Cochylls hospes, Coliaseurytheme, Corcyra cephalonica, Cydia latiferreanus, Cydia pomonella,Datana integerrima, Dendrolimus sibericus, Desmiafeneralis, Diaphaniahyalinata, Diaphania nitidalis, Diatraea grandiosella, Diatraeasaccharalis, Ennomos subsignaria, Eoreuma loftini, Esphestia elutella,Erannis tilaria, Estigmene acrea, Eulia salubricola, Eupocoelliaambiguella, Eupoecilia ambiguella, Euproctis chrysorrhoea, Euxoamessoria, Galleria mellonela, Grapholita molesta, Harrisina americana,Helicoverpa subflexa, Helicoverpa zea, Heliothis virescens, Hemileucaoliviae, Homoeosoma electellum, Hyphantia cunea, Keiferialycopersicella, Lambdina fiscellaria fiscellaria, Lambdina fiscellarialugubrosa, Leucoma salicis, Lobesia botrana, Loxostege sticticalis,Lymantria dispar, Macalla thyrisalis, Malacosoma sp., Mamestrabrassicae, Mamestra configurata, Manduca quinquemaculata, Manduca sexta,Maruca testulalis, Melanchra picta, Operophtera brumata, Orgyia sp.,Ostrinia nubilalis, Paleacrita vernata, Papilio cresphontes,Pectinophora gossypiella, Phryganidia californica, Phyllonorycterblancardella, Pieris napi, Pieris rapae, Plathypena scabra, Platynotaflouendana, Platynota stultana, Platyptilia carduidactyla, Plodiainterpunctella, Plutella xylostella, Pontia protodice, Pseudaletiaunipuncta, Pseudoplasia includens, Sabulodes aegrotata, Schizuraconcinna, Sitotroga cerealella, Spilona ocellana, Spodoptera sp.,Thaurnstopoea pityocampa, Tinsola bisselliella, Trichoplusia hi, Udearubigalis, Xylomyges curiails, and Yponomeuta padella.

Insect pests also include insects selected from the orders Diptera,Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera,Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera,Coleoptera. Insect pests of the invention for the major crops include,but are not limited to: Maize: Ostrinia nubilalis, European corn borer;Agrotis ipsilon, black cutworm; Helicoverpa zeae, corn earworm;Spodoptera frugiperda, fall armyworm; Diatraea grandiosella,southwestern corn borer; Elasmopalpus lignosellus, lesser cornstalkborer; Diatraea saccharalis, surgarcane borer; western corn rootworm,e.g., Diabrotica virgifera virgifera; northern corn rootworm, e.g.,Diabrotica longicornis barberi; southern corn rootworm, e.g., Diabroticaundecimpunctata howardi; Melanotus spp., wireworms; Cyclocephalaborealis, northern masked chafer (white grub); Cyclocephala immaculata,southern masked chafer (white grub); Popillia japonica, Japanese beetle;Chaetocnema pulicaria, corn flea beetle; Sphenophorus maidis, maizebillbug; Rhopalosiphum maidis, corn leaf aphid; Anuraphis maidiradicis,corn root aphid; Euschistus heros (Neotropical brown stink bug OR soystink bug); Piezodorus guildinii (red-banded stink bug); Dichelopsmelacanthus (no common name); Dichelops furcatus (no common name);Blissus leucopterus leucopterus, chinch bug; Melanoplus femurrubrum,redlegged grasshopper; Melanoplus sanguinipes, migratory grasshopper;Hylemya platura, seedcorn maggot; Agromyza parvicornis, corn blotchleafminer; Anaphothrips obscrurus, grass Thrips; Solenopsis milesta,thief ant; Tetranychus urticae, two spotted spider mite; Sorghum: Chilopartellus, sorghum borer; Spodoptera frugiperda, fall armyworm;Helicoverpa zea, corn earworm; Elasmopalpus lignosellus, leser cornstalkborer; Feltia subterranea, granulate cutworm; Phyllophaga crinita, whitegrub; Eleodes, Conoderus, and Aeolus spp., wireworms; Oulema melanopus,cereal leaf beetle; Chaetocnena pulicaria, corn flea beetle;Sphentophorus maidis, maize billbug; Rhopalosiphum maidis; corn leafaphid; Sipha flava, yellow sugarcane aphid; chinch bug, e.g., Blissusleucopterus leucopterus; Contarinia sorghicola, sorghum midge;Tetranychus cinnabarinus, carmine spider mite; Tetranychus urticae,two-spotted spider mite; Wheat: Pseudaletia unipunctata, army worm;Spodoptera frugiperda, fall armyworm; Elasmopalpus lignosellus, lessercornstalk borer; Agrotis orthogonia, pale western cutworm; Elasmopalpuslignosellus, lesser cornstalk borer; Oulema melanopus, cereal leafbeetle; Hypera punctata, clover leaf weevil; southern corn rootworm,e.g., Diabrotica undecimpunctata howardi; Russian wheat aphid;Schizaphis gramimum, greenbug; Macrosiphum avenae, English grain aphid;Melanoplus femurrubrum, redlegged grasshopper; Melanoplusdifferentialis, differential grasshopper; Melanoplus sanguinipes,migratory grasshopper; Mayetiola destructor, Hessian fly; Sitodiplosismosellana, wheat midge; Meromyza americana, wheat stem maggot; Hylemyacoarctata, wheat bulb fly; Frankliniella fusca, tobacco Thrips; Cephuscinctus, wheat stem sawfly; Aceria tulipae, wheat curl mite; Sunflower:Cylindrocupturus adspersus, sunflower stem weevil; Smicronyx fulus, redsunflower seed weevil; Smicronyx sordidus, gray sunflower seed weevil;Suleima helianthana, sunflower bud moth; Homoeosoma electellum,sunflower moth; Zygogramma exclamationis, sunflower beetle; Bothyrusgibbosus, carrot beetle; Neolasioplera murtfeldtiana, sunflower seedmidge; Cotton: Heliothis virescens, tobacco budworm; Helicoverpa zea,cotton bollworm; Spodoptera exigua, beet armyworm; Pectinophoragossypiella, pink bollworm; boll weevil, e.g., Anthonomus grandis; Aphisgossypii, cotton aphid; Pseudatomoscelis seriatus, cotton fleahopper;Trialeurodes abutilonea, bandedwinged whitefly; Lygus lineolaris,tarnished plant bug; Melanoplus femurrubrum, redlegged grasshopper;Melanoplus differentialis, differential grasshopper; Thrips tabaci,onion Thrips; Franklinkiella fusca, tobacco Thrips; Tetranychuscinnabarinus, carmine spider mite; Tetranychus urticae, two-spottedspider mite; Rice: Diatraea saccharalis, sugarcane borer; Spodopterafrugiperda, fall armyworm; Helicoverpa zea, corn earworm; Colaspisbrunnea, grape Colaspis; Lissorhoptrus oryzophilus, rice water weevil;Sitophilus oryzae, rice weevil; Nephotettix nigropictus, rice leafhoper;chinch bug, e.g., Blissus leucopterus leucopterus; Acrosternum hilare,green stink bug; Soybean: Pseudoplusia includens, soybean looper;Anticarsia gemmatalis, velvetbean caterpillar; Plathypena scabra, greencloverworm; Ostrinia nubilalis, European corn borer; Agrotis ipsilon,black cutworm; Spodoptera exigua, beet armyworm; Heliothis virescens,tobacco budworm; Helicoverpa zea, cotton bollworm; Epilachna varivestis,Mexican bean beetle; Myzus persicae, green peach aphid; Empoasca fabae,potato leafhopper; Acrosternum hilare, green stink bug; Melanoplusfemurrubrum, redlegged grasshopper; Melanoplus differentialis,differential grasshopper; Hylemya platura, seedcorn maggot; Sericothripsvariabilis, soybean Thrips; Thrips tabaci, onion Thrips; Tetranychusturkestani, strawberry spider mite; Tetranychus urticae, two-spottedspider mite; Barley: Ostrinia nubilalis, European corn borer; Agrotisipsilon, black cutworm; Schizaphis graminum, greenbug; chinch bug, e.g.,Blissus leucopterus leucopterus; Acrosternum hilare, green stink bug;Euschistus servus, brown stink bug; Jylemya platura, seedcorn maggot;Mayetiola destructor, Hessian fly; Petrobia latens, brown wheat mite;Oil Seed Rape: Vrevicoryne brassicae, cabbage aphid; Phyllotretacruciferae, crucifer flea beetle; Phyllotreta striolata, striped fleabeetle; Phyllotreta nemorum, striped turnip flea beetle; Meligethesaeneus, rapeseed beetle; and the pollen beetles Meligethes rufimanus,Meligethes nigrescens, Meligethes canadianus, and Meligethesviridescens; Potato: Leptinotarsa decemlineata, Colorado potato beetle.

The methods and compositions provided herein may be effective againstHemiptera such as Lygus hesperus, Lygus lineolaris, Lygus pratensis,Lygus rugulipennis Popp, Lygus pabulinus, Calocoris norvegicus, Orthopscompestris, Plesiocoris rugicollis, Cyrtopeltis modestus, Cyrtopeltisnotatus, Spanagonicus albofasciatus, Diaphnocoris chlorinonis,Labopidicola allii, Pseudatomoscelis seriatus, Adelphocoris rapidus,Poecilocapsus lineatus, Blissus leucopterus, Nysius ericae, Nysiusraphanus, Euschistus servus, Nezara viridula, Eurygaster, Coreidae,Pyrrhocoridae, Tinidae, Blostomatidae, Reduviidae, and Cimicidae. Pestsof interest also include Araecerus fasciculatus, coffee bean weevil;Acanthoscelides obtectus, bean weevil; Bruchus rufmanus, broadbeanweevil; Bruchus pisorum, pea weevil; Zabrotes subfasciatus, Mexican beanweevil; Diabrotica balteata, banded cucumber beetle; Cerotomatrifurcata, bean leaf beetle; Diabrotica virgifera, Mexican cornrootworm; Epitrix cucumeris, potato flea beetle; Chaetocnema confinis,sweet potato flea beetle; Hypera postica, alfalfa weevil; Anthonomusquadrigibbus, apple curculio; Sternechus paludatus, bean stalk weevil;Hypera brunnipennis, Egyptian alfalfa weevil; Sitophilus granaries,granary weevil; Craponius inaequalis, grape curculio; Sitophiluszeamais, maize weevil; Conotrachelus nenuphar, plum curculio; Euscepespostfaciatus, West Indian sweet potato weevil; Maladera castanea,Asiatic garden beetle; Rhizotrogus majalis, European chafer;Macrodactylus subspinosus, rose chafer; Tribolium confusum, confusedflour beetle; Tenebrio obscurus, dark mealworm; Tribolium castaneum, redflour beetle; Tenebrio molitor, yellow mealworm.

Nematodes include parasitic nematodes such as root-knot, cyst, andlesion nematodes, including Heterodera spp., Meloidogyne spp., andGlobodera spp.; particularly members of the cyst nematodes, including,but not limited to, Heterodera glycines (soybean cyst nematode);Heterodera schachtii (beet cyst nematode); Heterodera avenae (cerealcyst nematode); and Globodera rostochiensis and Globodera pallida(potato cyst nematodes). Lesion nematodes include Pratylenchus spp.

Insect pests may be tested for pesticidal activity of compositions ofthe invention in early developmental stages, e.g., as larvae or otherimmature forms. The insects may be reared in total darkness at fromabout 20 .degree. C. to about 30 .degree. C. and from about 30% to about70% relative humidity. Bioassays may be performed as described in Czaplaand Lang (1990) J. Econ. Entomol. 83 (6): 2480-2485. See, also theexperimental section herein.

III. Expression Cassettes

Polynucleotides encoding the pesticidal proteins provided herein can beprovided in expression cassettes for expression in an organism ofinterest. The cassette will include 5′ and 3′ regulatory sequencesoperably linked to a polynucleotide encoding a pesticidal polypeptideprovided herein that allows for expression of the polynucleotide. Thecassette may additionally contain at least one additional gene orgenetic element to be cotransformed into the organism. Where additionalgenes or elements are included, the components are operably linked.Alternatively, the additional gene(s) or element(s) can be provided onmultiple expression cassettes. Such an expression cassette is providedwith a plurality of restriction sites and/or recombination sites forinsertion of the polynucleotides to be under the transcriptionalregulation of the regulatory regions. The expression cassette mayadditionally contain a selectable marker gene.

The expression cassette will include in the 5′-3′ direction oftranscription, a transcriptional and translational initiation region(i.e., a promoter), a pesticidal polynucleotide of the invention, and atranscriptional and translational termination region (i.e., terminationregion) functional in the organism of interest, i.e., a plant orbacteria. The promoters of the invention are capable of directing ordriving expression of a coding sequence in a host cell. The regulatoryregions (i.e., promoters, transcriptional regulatory regions, andtranslational termination regions) may be endogenous or heterologous tothe host cell or to each other. As used herein, “heterologous” inreference to a sequence is a sequence that originates from a foreignspecies, or, if from the same species, is substantially modified fromits native form in composition and/or genomic locus by deliberate humanintervention. As used herein, a chimeric gene comprises a codingsequence operably linked to a transcription initiation region that isheterologous to the coding sequence.

Convenient termination regions are available from the Ti-plasmid of A.tumefaciens, such as the octopine synthase and nopaline synthasetermination regions. See also Guerineau et al. (1991) Mol. Gen. Genet.262:141-144; Proudfoot (1991) Cell 64:671-674; Sanfacon et al. (1991)Genes Dev. 5:141-149; Mogen et al. (1990) Plant Cell 12:1261-1272;Munroe et al. (1990) Gene 91:151-158; Ballas et al. (1989) Nucleic AcidsRes. 17:7891-7903; and Joshi et al. (1987) Nucleic Acids Res.15:9627-9639.

Additional regulatory signals include, but are not limited to,transcriptional initiation start sites, operators, activators,enhancers, other regulatory elements, ribosomal binding sites, aninitiation codon, termination signals, and the like. See, for example,U.S. Pat. Nos. 5,039,523 and 4,853,331; EPO 0480762A2; Sambrook et al.(1992) Molecular Cloning: A Laboratory Manual, ed. Maniatis et al. (ColdSpring Harbor Laboratory Press, Cold Spring Harbor, N.Y.), hereinafter“Sambrook 11”; Davis et al., eds. (1980) Advanced Bacterial Genetics(Cold Spring Harbor Laboratory Press), Cold Spring Harbor, N.Y., and thereferences cited therein.

In preparing the expression cassette, the various DNA fragments may bemanipulated, so as to provide for the DNA sequences in the properorientation and, as appropriate, in the proper reading frame. Towardthis end, adapters or linkers may be employed to join the DNA fragmentsor other manipulations may be involved to provide for convenientrestriction sites, removal of superfluous DNA, removal of restrictionsites, or the like. For this purpose, in vitro mutagenesis, primerrepair, restriction, annealing, resubstitutions, e.g., transitions andtransversions, may be involved.

A number of promoters can be used in the practice of the invention. Thepromoters can be selected based on the desired outcome. The nucleicacids can be combined with constitutive, inducible, tissue-preferred, orother promoters for expression in the organism of interest. See, forexample, promoters set forth in WO 99/43838 and in U.S. Pat. Nos.8,575,425; 7,790,846; 8,147,856; 8,586,832; 7,772,369; 7,534,939;6,072,050; 5,659,026; 5,608,149; 5,608,144; 5,604,121; 5,569,597;5,466,785; 5,399,680; 5,268,463; 5,608,142; and 6,177,611; hereinincorporated by reference.

For expression in plants, constitutive promoters also include CaMV 35Spromoter (Odell et al. (1985) Nature 313:810-812); rice actin (McElroyet al. (1990) Plant Cell 2:163-171); ubiquitin (Christensen et al.(1989) Plant Mol. Biol. 12:619-632 and Christensen et al. (1992) PlantMol. Biol. 18:675-689); pEMU (Last et al. (1991) Theor. Appl. Genet.81:581-588); MAS (Velten et al. (1984) EMBO J. 3:2723-2730). Induciblepromoters include those that drive expression of pathogenesis-relatedproteins (PR proteins), which are induced following infection by apathogen. See, for example, Redolfi et al. (1983) Neth. J. Plant Pathol.89:245-254; Uknes et al. (1992) Plant Cell 4:645-656; and Van Loon(1985) Plant Mol. Virol. 4:111-116; and WO 99/43819, herein incorporatedby reference. Promoters that are expressed locally at or near the siteof pathogen infection may also be used (Marineau et al. (1987) PlantMol. Biol. 9:335-342; Matton et al. (1989) Molecular Plant-MicrobeInteractions 2:325-331; Somsisch et al. (1986) Proc. Natl. Acad. Sci.USA 83:2427-2430; Somsisch et al. (1988) Mol. Gen. Genet. 2:93-98; andYang (1996) Proc. Natl. Acad. Sci. USA 93:14972-14977; Chen et al.(1996) Plant J. 10:955-966; Zhang et al. (1994) Proc. Natl. Acad. Sci.USA 91:2507-2511; Warner et al. (1993) Plant J. 3:191-201; Siebertz etal. (1989) Plant Cell 1:961-968; Cordero et al. (1992) Physiol. Mol.Plant Path. 41:189-200; U.S. Pat. No. 5,750,386 (nematode-inducible);and the references cited therein).

Wound-inducible promoters may be used in the constructions of theinvention. Such wound-inducible promoters include pin II promoter (Ryan(1990) Ann. Rev. Phytopath. 28:425-449; Duan et al. (1996) NatureBiotechnology 14:494-498); wun1 and wun2 (U.S. Pat. No. 5,428,148); win1and win2 (Stanford et al. (1989) Mol. Gen. Genet. 215:200-208); systemin(McGurl et al. (1992) Science 225:1570-1573); WIP1 (Rohmeier et al.(1993) Plant Mol. Biol. 22:783-792; Eckelkamp et al. (1993) FEBS Letters323:73-76); MPI gene (Corderok et al. (1994) Plant J. 6(2):141-150); andthe like, herein incorporated by reference.

Tissue-preferred promoters for use in the invention include those setforth in Yamamoto et al. (1997) Plant J. 12(2):255-265; Kawamata et al.(1997) Plant Cell Physiol. 38(7):792-803; Hansen et al. (1997) Mol. GenGenet. 254(3):337-343; Russell et al. (1997) Transgenic Res.6(2):157-168; Rinehart et al. (1996) Plant Physiol. 112(3):1331-1341;Van Camp et al. (1996) Plant Physiol. 112(2):525-535; Canevascini et al.(1996) Plant Physiol. 112(2):513-524; Yamamoto et al. (1994) Plant CellPhysiol. 35(5):773-778; Lam (1994) Results Probl. Cell Differ.20:181-196; Orozco et al. (1993) Plant Mol Biol. 23(6):1129-1138;Matsuoka et al. (1993) Proc Natl. Acad. Sci. USA 90(20):9586-9590; andGuevara-Garcia et al. (1993) Plant J. 4(3):495-505.

Leaf-preferred promoters include those set forth in Yamamoto et al.(1997) Plant J. 12(2):255-265; Kwon et al. (1994) Plant Physiol.105:357-67; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778;Gotor et al. (1993) Plant J. 3:509-18; Orozco et al. (1993) Plant Mol.Biol. 23(6):1129-1138; and Matsuoka et al. (1993) Proc. Natl. Acad. Sci.USA 90(20):9586-9590.

Root-preferred promoters are known and include those in Hire et al.(1992) Plant Mol. Biol. 20(2):207-218 (soybean root-specific glutaminesynthetase gene); Keller and Baumgartner (1991) Plant Cell3(10):1051-1061 (root-specific control element); Sanger et al. (1990)Plant Mol. Biol. 14(3):433-443 (mannopine synthase (MAS) gene ofAgrobacterium tumefaciens); and Miao et al. (1991) Plant Cell 3(1):11-22(cytosolic glutamine synthetase (GS)); Bogusz et al. (1990) Plant Cell2(7):633-641; Leach and Aoyagi (1991) Plant Science (Limerick)79(1):69-76 (rolC and rolD); Teeri et al. (1989) EMBO J. 8(2):343-350;Kuster et al. (1995) Plant Mol. Biol. 29(4):759-772 (the VfENOD-GRP3gene promoter); and, Capana et al. (1994) Plant Mol. Biol. 25(4):681-691(rolB promoter). See also U.S. Pat. Nos. 5,837,876; 5,750,386;5,633,363; 5,459,252; 5,401,836; 5,110,732; and U.S. Pat. No. 5,023,179.

“Seed-preferred” promoters include both “seed-specific” promoters (thosepromoters active during seed development such as promoters of seedstorage proteins) as well as “seed-germinating” promoters (thosepromoters active during seed germination). See Thompson et al. (1989)BioEssays 10:108. Seed-preferred promoters include, but are not limitedto, Cim1 (cytokinin-induced message); cZ19B1 (maize 19 kDa zein); milps(myo-inositol-1-phosphate synthase) (see WO 00/11177 and U.S. Pat. No.6,225,529). Gamma-zein is an endosperm-specific promoter. Globulin 1(Glb-1) is a representative embryo-specific promoter. For dicots,seed-specific promoters include, but are not limited to, beanβ-phaseolin, napin, β-conglycinin, soybean lectin, cruciferin, and thelike. For monocots, seed-specific promoters include, but are not limitedto, maize 15 kDa zein, 22 kDa zein, 27 kDa zein, gamma-zein, waxy,shrunken 1, shrunken 2, Globulin 1, etc. See also WO 00/12733, whereseed-preferred promoters from end1 and end2 genes are disclosed.

For expression in a bacterial host, promoters that function in bacteriaare well-known in the art. Such promoters include any of the knowncrystal protein gene promoters, including the promoters of any of thepesticidal proteins of the invention, and promoters specific for B.thuringiensis sigma factors. Alternatively, mutagenized or recombinantcrystal protein-encoding gene promoters may be recombinantly engineeredand used to promote expression of the novel gene segments disclosedherein.

The expression cassette can also comprise a selectable marker gene forthe selection of transformed cells. Selectable marker genes are utilizedfor the selection of transformed cells or tissues. Marker genes includegenes encoding antibiotic resistance, such as those encoding neomycinphosphotransferase II (NEO) and hygromycin phosphotransferase (HPT), aswell as genes conferring resistance to herbicidal compounds, such asglufosinate ammonium, bromoxynil, imidazolinones, and2,4-dichlorophenoxyacetate (2,4-D). Additional selectable markers areknown and any can be used. See, for example, U.S. Provisionalapplication 62/094,697, filed on Dec. 19, 2014, and U.S. ProvisionalApplication 62/189,505, filed Jul. 7, 2015, both of which are hereinincorporated by reference in their entirety, which discloses glufosinateresistance sequences that can be employed as selectable markers. See,for example, PCT/US2015/066648, filed on Dec. 18, 2015, hereinincorporated by reference in its entirety, which discloses glufosinateresistance sequences that can be employed as selectable markers.

IV. Methods, Host Cells and Plant Cells

As indicated, DNA constructs comprising nucleotide sequences encodingthe pesticidal proteins or active variants or fragment thereof can beused to transform plants of interest or other organisms of interest.Methods for transformation involve introducing a nucleotide constructinto a plant. By “introducing” is intended to introduce the nucleotideconstruct to the plant or other host cell in such a manner that theconstruct gains access to the interior of a cell of the plant or hostcell. The methods of the invention do not require a particular methodfor introducing a nucleotide construct to a plant or host cell, onlythat the nucleotide construct gains access to the interior of at leastone cell of the plant or the host organism. Methods for introducingnucleotide constructs into plants and other host cells are known in theart including, but not limited to, stable transformation methods,transient transformation methods, and virus-mediated methods.

The methods result in a transformed organisms, such as a plant,including whole plants, as well as plant organs (e.g., leaves, stems,roots, etc.), seeds, plant cells, propagules, embryos and progeny of thesame. Plant cells can be differentiated or undifferentiated (e.g.callus, suspension culture cells, protoplasts, leaf cells, root cells,phloem cells, pollen).

“Transgenic plants” or “transformed plants” or “stably transformed”plants or cells or tissues refers to plants that have incorporated orintegrated a polynucleotide encoding at least one pesticidal polypeptideof the invention. It is recognized that other exogenous or endogenousnucleic acid sequences or DNA fragments may also be incorporated intothe plant cell. Agrobacterium- and biolistic-mediated transformationremain the two predominantly employed approaches. However,transformation may be performed by infection, transfection,microinjection, electroporation, microprojection, biolistics or particlebombardment, electroporation, silica/carbon fibers, ultrasound mediated,PEG mediated, calcium phosphate co-precipitation, polycation DMSOtechnique, DEAE dextran procedure, Agro and viral mediated(Caulimoriviruses, Geminiviruses, RNA plant viruses), liposome mediatedand the like.

Transformation protocols as well as protocols for introducingpolypeptides or polynucleotide sequences into plants may vary dependingon the type of plant or plant cell, i.e., monocot or dicot, targeted fortransformation. Methods for transformation are known in the art andinclude those set forth in U.S. Pat. Nos. 8,575,425; 7,692,068;8,802,934; 7,541,517; each of which is herein incorporated by reference.See, also, Rakoczy-Trojanowska, M. (2002) Cell Mol Biol Lett. 7:849-858;Jones et al. (2005) Plant Methods 1:5; Rivera et al. (2012) Physics ofLife Reviews 9:308-345; Bartlett et al. (2008) Plant Methods 4:1-12;Bates, G. W. (1999) Methods in Molecular Biology 111:359-366; Binns andThomashow (1988) Annual Reviews in Microbiology 42:575-606; Christou, P.(1992) The Plant Journal 2:275-281; Christou, P. (1995) Euphytica85:13-27; Tzfira et al. (2004) TRENDS in Genetics 20:375-383; Yao et al.(2006) Journal of Experimental Botany 57:3737-3746; Zupan and Zambryski(1995) Plant Physiology 107:1041-1047; Jones et al. (2005) Plant Methods1:5;

Transformation may result in stable or transient incorporation of thenucleic acid into the cell. “Stable transformation” is intended to meanthat the nucleotide construct introduced into a host cell integratesinto the genome of the host cell and is capable of being inherited bythe progeny thereof. “Transient transformation” is intended to mean thata polynucleotide is introduced into the host cell and does not integrateinto the genome of the host cell.

Methods for transformation of chloroplasts are known in the art. See,for example, Svab et al. (1990) Proc. Nail. Acad. Sci. USA 87:8526-8530;Svab and Maliga (1993) Proc. Natl. Acad. Sci. USA 90:913-917; Svab andMaliga (1993) EMBO J. 12:601-606. The method relies on particle gundelivery of DNA containing a selectable marker and targeting of the DNAto the plastid genome through homologous recombination. Additionally,plastid transformation can be accomplished by transactivation of asilent plastid-borne transgene by tissue-preferred expression of anuclear-encoded and plastid-directed RNA polymerase. Such a system hasbeen reported in McBride et al. (1994) Proc. Natl. Acad. Sci. USA91:7301-7305.

The cells that have been transformed may be grown into plants inaccordance with conventional ways. See, for example, McCormick et al.(1986) Plant Cell Reports 5:81-84. These plants may then be grown, andeither pollinated with the same transformed strain or different strains,and the resulting hybrid having constitutive expression of the desiredphenotypic characteristic identified. Two or more generations may begrown to ensure that expression of the desired phenotypic characteristicis stably maintained and inherited and then seeds harvested to ensureexpression of the desired phenotypic characteristic has been achieved.In this manner, the present invention provides transformed seed (alsoreferred to as “transgenic seed”) having a nucleotide construct of theinvention, for example, an expression cassette of the invention, stablyincorporated into their genome.

In specific embodiments, the sequences provide herein can be targeted tospecific cite within the genome of the host cell or plant cell. Suchmethods include, but are not limited to, meganucleases designed againstthe plant genomic sequence of interest (D'Halluin et al. 2013 PlantBiotechnol J); CRISPR-Cas9, TALENs, and other technologies for preciseediting of genomes (Feng, et al. Cell Research 23:1229-1232, 2013,Podevin, et al. Trends Biotechnology, online publication, 2013, Wei etal., J Gen Genomics, 2013, Zhang et al (2013) WO 2013/026740); Cre-loxsite-specific recombination (Dale et al. (1995) Plant J 7:649-659;Lyznik, et al. (2007) Transgenic Plant J 1:1-9; FLP-FRT recombination(Li et al. (2009) Plant Physiol 151:1087-1095); Bxb1-mediatedintegration (Yau et al. Plant J (2011) 701:147-166); zinc-fingermediated integration (Wright et al. (2005) Plant J 44:693-705); Cai etal. (2009) Plant Mol Biol 69:699-709); and homologous recombination(Lieberman-Lazarovich and Levy (2011) Methods Mol Biol 701: 51-65);Puchta (2002) Plant Mol Biol 48:173-182).

The sequence provided herein may be used for transformation of any plantspecies, including, but not limited to, monocots and dicots. Examples ofplants of interest include, but are not limited to, corn (maize),sorghum, wheat, sunflower, tomato, crucifers, peppers, potato, cotton,rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape,Brassica sp., alfalfa, rye, millet, safflower, peanuts, sweet potato,cassaya, coffee, coconut, pineapple, citrus trees, cocoa, tea, banana,avocado, fig, guava, mango, olive, papaya, cashew, macadamia, almond,oats, vegetables, ornamentals, and conifers.

Vegetables include, but are not limited to, tomatoes, lettuce, greenbeans, lima beans, peas, and members of the genus Curcumis such ascucumber, cantaloupe, and musk melon. Ornamentals include, but are notlimited to, azalea, hydrangea, hibiscus, roses, tulips, daffodils,petunias, carnation, poinsettia, and chrysanthemum. Preferably, plantsof the present invention are crop plants (for example, maize, sorghum,wheat, sunflower, tomato, crucifers, peppers, potato, cotton, rice,soybean, sugarbeet, sugarcane, tobacco, barley, oilseed rape, etc.).

As used herein, the term plant includes plant cells, plant protoplasts,plant cell tissue cultures from which plants can be regenerated, plantcalli, plant clumps, and plant cells that are intact in plants or partsof plants such as embryos, pollen, ovules, seeds, leaves, flowers,branches, fruit, kernels, ears, cobs, husks, stalks, roots, root tips,anthers, and the like. Grain is intended to mean the mature seedproduced by commercial growers for purposes other than growing orreproducing the species. Progeny, variants, and mutants of theregenerated plants are also included within the scope of the invention,provided that these parts comprise the introduced polynucleotides.Further provided is a processed plant product or byproduct that retainsthe sequences disclosed herein, including for example, soymeal.

In another embodiment, the genes encoding the pesticidal proteins can beused to transform insect pathogenic organisms. Such organisms includebaculoviruses, fungi, protozoa, bacteria, and nematodes. Microorganismhosts that are known to occupy the “phytosphere” (phylloplane,phyllosphere, rhizosphere, and/or rhizoplana) of one or more crops ofinterest may be selected. These microorganisms are selected so as to becapable of successfully competing in the particular environment with thewild-type microorganisms, provide for stable maintenance and expressionof the gene expressing the pesticidal protein, and desirably, providefor improved protection of the pesticide from environmental degradationand inactivation.

Such microorganisms include archaea, bacteria, algae, and fungi. Ofparticular interest are microorganisms such as bacteria, e.g., Bacillus,Pseudomonas, Erwinia, Serratia, Klebsiella, Xanthomonas, Streptomyces,Rhizobium, Rhodopseudomonas, Methylius, Agrobacterium, Acetobacter,Lactobacillus, Arthrobacter, Azotobacter, Leuconostoc, and Alcaligenes.Fungi include yeast, e.g., Saccharomyces, Cryptococcus, Kluyveromyces,Sporobolomyces, Rhodotorula, and Aureobasidium. Of particular interestare such phytosphere bacterial species as Pseudomonas syringae,Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens,Acetobacter xylinum, Agrobacteria, Rhodopseudomonas spheroides,Xanthomonas campestris, Rhizobium melioti, Alcaligenes entrophus,Clavibacter xyli and Azotobacter vinlandir and phytosphere yeast speciessuch as Rhodotorula rubra, R. glutinis, R. marina, R. aurantiaca,Cryptococcus albidus, C. diffluens, C. laurentii, Saccharomyces rosei,S. pretoriensis, S. cerevisiae, Sporobolomyces rosues, S. odorus,Kluyveromyces veronae, Aureobasidium pollulans, Bacillus thuringiensis,Escherichia coli, Bacillus subtilis, and the like.

Illustrative prokaryotes, both Gram-negative and gram-positive, includeEnterobacteriaceae, such as Escherichia, Erwinia, Shigella, Salmonella,and Proteus; Bacillaceae; Rhizobiceae, such as Rhizobium; Spirillaceae,such as photobacterium, Zymomonas, Serratia, Aeromonas, Vibrio,Desulfovibrio, Spirillum; Lactobacillaceae; Pseudomonadaceae, such asPseudomonas and Acetobacter; Azotobacteraceae and Nitrobacteraceae.Fungi include Phycomycetes and Ascomycetes, e.g., yeast, such asSaccharomyces and Schizosaccharomyces; and Basidiomycetes yeast, such asRhodotorula, Aureobasidium, Sporobolomyces, and the like.

Genes encoding pesticidal proteins can be introduced by means ofelectrotransformation, PEG induced transformation, heat shock,transduction, conjugation, and the like. Specifically, genes encodingthe pesticidal proteins can be cloned into a shuttle vector, forexample, pHT3101 (Lerecius et al. (1989) FEMS Microbiol. Letts. 60:211-218. The shuttle vector pHT3101 containing the coding sequence forthe particular pesticidal protein gene can, for example, be transformedinto the root-colonizing Bacillus by means of electroporation (Lereciuset al. (1989) FEMS Microbiol. Letts. 60: 211-218).

Expression systems can be designed so that pesticidal proteins aresecreted outside the cytoplasm of gram-negative bacteria by fusing anappropriate signal peptide to the amino-terminal end of the pesticidalprotein. Signal peptides recognized by E. coli include the OmpA protein(Ghrayeb et al. (1984) EMBO J, 3: 2437-2442).

Pesticidal proteins and active variants thereof can be fermented in abacterial host and the resulting bacteria processed and used as amicrobial spray in the same manner that Bacillus thuringiensis strainshave been used as insecticidal sprays. In the case of a pesticidalprotein(s) that is secreted from Bacillus, the secretion signal isremoved or mutated using procedures known in the art. Such mutationsand/or deletions prevent secretion of the pesticidal protein(s) into thegrowth medium during the fermentation process. The pesticidal proteinsare retained within the cell, and the cells are then processed to yieldthe encapsulated pesticidal proteins.

Alternatively, the pesticidal proteins are produced by introducingheterologous genes into a cellular host. Expression of the heterologousgene results, directly or indirectly, in the intracellular productionand maintenance of the pesticide. These cells are then treated underconditions that prolong the activity of the toxin produced in the cellwhen the cell is applied to the environment of target pest(s). Theresulting product retains the toxicity of the toxin. These naturallyencapsulated pesticidal proteins may then be formulated in accordancewith conventional techniques for application to the environment hostinga target pest, e.g., soil, water, and foliage of plants. See, forexample U.S. Pat. No. 6,468,523 and U.S. Publication No. 20050138685,and the references cited therein. In the present invention, atransformed microorganism (which includes whole organisms, cells,spore(s), pesticidal protein(s), pesticidal component(s), pest-impactingcomponent(s), mutant(s), living or dead cells and cell components,including mixtures of living and dead cells and cell components, andincluding broken cells and cell components) or an isolated pesticidalprotein can be formulated with an acceptable carrier into a pesticidalor agricultural composition(s) that is, for example, a suspension, asolution, an emulsion, a dusting powder, a dispersible granule, awettable powder, and an emulsifiable concentrate, an aerosol, animpregnated granule, an adjuvant, a coatable paste, and alsoencapsulations in, for example, polymer substances.

Agricultural compositions may comprise a polypeptide, a recombinogenicpolypeptide or a variant or fragment thereof, as disclosed herein. Theagricultural composition disclosed herein may be applied to theenvironment of a plant or an area of cultivation, or applied to theplant, plant part, plant cell, or seed.

Such compositions disclosed above may be obtained by the addition of asurface-active agent, an inert carrier, a preservative, a humectant, afeeding stimulant, an attractant, an encapsulating agent, a binder, anemulsifier, a dye, a UV protectant, a buffer, a flow agent orfertilizers, micronutrient donors, or other preparations that influenceplant growth. One or more agrochemicals including, but not limited to,herbicides, insecticides, fungicides, bactericides, nematicides,molluscicides, acaricides, plant growth regulators, harvest aids, andfertilizers, can be combined with carriers, surfactants or adjuvantscustomarily employed in the art of formulation or other components tofacilitate product handling and application for particular target pests.Suitable carriers and adjuvants can be solid or liquid and correspond tothe substances ordinarily employed in formulation technology, e.g.,natural or regenerated mineral substances, solvents, dispersants,wetting agents, tackifiers, binders, or fertilizers. The activeingredients of the present invention are normally applied in the form ofcompositions and can be applied to the crop area, plant, or seed to betreated. For example, the compositions of the present invention may beapplied to grain in preparation for or during storage in a grain bin orsilo, etc. The compositions of the present invention may be appliedsimultaneously or in succession with other compounds. Methods ofapplying an active ingredient of the present invention or anagrochemical composition of the present invention that contains at leastone of the pesticidal proteins produced by the bacterial strains of thepresent invention include, but are not limited to, foliar application,seed coating, and soil application. The number of applications and therate of application depend on the intensity of infestation by thecorresponding pest.

Suitable surface-active agents include, but are not limited to, anioniccompounds such as a carboxylate of, for example, a metal; a carboxylateof a long chain fatty acid; an N-acylsarcosinate; mono or di-esters ofphosphoric acid with fatty alcohol ethoxylates or salts of such esters;fatty alcohol sulfates such as sodium dodecyl sulfate, sodium octadecylsulfate or sodium cetyl sulfate; ethoxylated fatty alcohol sulfates;ethoxylated alkylphenol sulfates; lignin sulfonates; petroleumsulfonates; alkyl aryl sulfonates such as alkyl-benzene sulfonates orlower alkylnaphthalene sulfonates, e.g., butyl-naphthalene sulfonate;salts of sulfonated naphthalene-formaldehyde condensates; salts ofsulfonated phenol-formaldehyde condensates; more complex sulfonates suchas the amide sulfonates, e.g., the sulfonated condensation product ofoleic acid and N-methyl taurine; or the dialkyl sulfosuccinates, e.g.,the sodium sulfonate of dioctyl succinate. Non-ionic agents includecondensation products of fatty acid esters, fatty alcohols, fatty acidamides or fatty-alkyl- or alkenyl-substituted phenols with ethyleneoxide, fatty esters of polyhydric alcohol ethers, e.g., sorbitan fattyacid esters, condensation products of such esters with ethylene oxide,e.g., polyoxyethylene sorbitar fatty acid esters, block copolymers ofethylene oxide and propylene oxide, acetylenic glycols such as2,4,7,9-tetraethyl-5-decyn-4,7-diol, or ethoxylated acetylenic glycols.Examples of a cationic surface-active agent include, for instance, analiphatic mono-, di-, or polyamine such as an acetate, naphthenate oroleate; or oxygen-containing amine such as an amine oxide ofpolyoxyethylene alkylamine; an amide-linked amine prepared by thecondensation of a carboxylic acid with a di- or polyamine; or aquaternary ammonium salt.

Examples of inert materials include but are not limited to inorganicminerals such as kaolin, phyllosilicates, carbonates, sulfates,phosphates, or botanical materials such as cork, powdered corncobs,peanut hulls, rice hulls, and walnut shells.

The compositions of the present invention can be in a suitable form fordirect application or as a concentrate of primary composition thatrequires dilution with a suitable quantity of water or other diluentbefore application. The pesticidal concentration will vary dependingupon the nature of the particular formulation, specifically, whether itis a concentrate or to be used directly. The composition contains 1 to98% of a solid or liquid inert carrier, and 0 to 50% or 0.1 to 50% of asurfactant. These compositions will be administered at the labeled ratefor the commercial product, for example, about 0.01 lb-5.0 lb. per acrewhen in dry form and at about 0.01 pts.-10 pts. per acre when in liquidform.

In a further embodiment, the compositions, as well as the transformedmicroorganisms and pesticidal proteins, provided herein can be treatedprior to formulation to prolong the pesticidal activity when applied tothe environment of a target pest as long as the pretreatment is notdeleterious to the pesticidal activity. Such treatment can be bychemical and/or physical means as long as the treatment does notdeleteriously affect the properties of the composition(s). Examples ofchemical reagents include but are not limited to halogenating agents;aldehydes such as formaldehyde and glutaraldehyde; anti-infectives, suchas zephiran chloride; alcohols, such as isopropanol and ethanol; andhistological fixatives, such as Bouin's fixative and Helly's fixative(see, for example, Humason (1967) Animal Tissue Techniques (W.H. Freemanand Co.).

In one aspect, pests may be killed or reduced in numbers in a given areaby application of the pesticidal proteins provided herein to the area.Alternatively, the pesticidal proteins may be prophylactically appliedto an environmental area to prevent infestation by a susceptible pest.Preferably the pest ingests, or is contacted with, apesticidally-effective amount of the polypeptide. By“pesticidally-effective amount” is intended an amount of the pesticidethat is able to bring about death to at least one pest, or to noticeablyreduce pest growth, feeding, or normal physiological development. Thisamount will vary depending on such factors as, for example, the specifictarget pests to be controlled, the specific environment, location,plant, crop, or agricultural site to be treated, the environmentalconditions, and the method, rate, concentration, stability, and quantityof application of the pesticidally-effective polypeptide composition.The formulations or compositions may also vary with respect to climaticconditions, environmental considerations, and/or frequency ofapplication and/or severity of pest infestation.

The active ingredients are normally applied in the form of compositionsand can be applied to the crop area, plant, or seed to be treated.Methods are therefore provided for providing to a plant, plant cell,seed, plant part or an area of cultivation, an effective amount of theagricultural composition comprising the polypeptide, recombinogenicpolypeptide or an active variant or fragment thereof. By “effectiveamount” is intended an amount of a protein or composition has pesticidalactivity that is sufficient to kill or control the pest or result in anoticeable reduction in pest growth, feeding, or normal physiologicaldevelopment. Such decreases in numbers, pest growth, feeding or normaldevelopment can comprise any statistically significant decrease,including, for example a decrease of about 5%, 10%, 15%, 20%, 25%, 30%,35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% or greater.

For example, the compositions may be applied to grain in preparation foror during storage in a grain bin or silo, etc. The compositions may beapplied simultaneously or in succession with other compounds. Methods ofapplying an active ingredient or an agrochemical composition comprisingat least one of the polypeptides, recombinogenic polypeptides orvariants or fragments thereof as disclosed herein, include but are notlimited to, foliar application, seed coating, and soil application.

Methods for increasing plant yield are provided. The methods compriseproviding a plant or plant cell expressing a polynucleotide encoding thepesticidal polypeptide sequence disclosed herein and growing the plantor a seed thereof in a field infested with (or susceptible toinfestation by) a pest against which said polypeptide has pesticidalactivity. In some embodiments, the polypeptide has pesticidal activityagainst a lepidopteran, coleopteran, dipteran, hemipteran, or nematodepest, and said field is infested with a lepidopteran, hemipteran,coleopteran, dipteran, or nematode pest. As defined herein, the “yield”of the plant refers to the quality and/or quantity of biomass producedby the plant. By “biomass” is intended any measured plant product. Anincrease in biomass production is any improvement in the yield of themeasured plant product. Increasing plant yield has several commercialapplications. For example, increasing plant leaf biomass may increasethe yield of leafy vegetables for human or animal consumption.Additionally, increasing leaf biomass can be used to increase productionof plant-derived pharmaceutical or industrial products. An increase inyield can comprise any statistically significant increase including, butnot limited to, at least a 1% increase, at least a 3% increase, at leasta 5% increase, at least a 10% increase, at least a 20% increase, atleast a 30%, at least a 50%, at least a 70%, at least a 100% or agreater increase in yield compared to a plant not expressing thepesticidal sequence. In specific methods, plant yield is increased as aresult of improved pest resistance of a plant expressing a pesticidalprotein disclosed herein. Expression of the pesticidal protein resultsin a reduced ability of a pest to infest or feed.

The plants can also be treated with one or more chemical compositions,including one or more herbicide, insecticides, or fungicides.

Non-limiting embodiments include:

1. An isolated polypeptide having insecticidal activity, comprising

-   -   (a) a polypeptide comprising an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID NO:        1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,        19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34,        35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,        51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,        67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82,        83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,        99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111,        112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,        125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,        138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,        151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163,        164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176,        177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,        190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,        203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215,        216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228,        229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,        242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,        255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,        268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,        281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293,        294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,        307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319,        320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,        333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345,        346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358,        359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,        372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,        385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397,        398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410,        411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,        424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,        437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,        450, 451, 452, 453, 454, 455, 456, 457, and/or 458; or    -   (b) a polypeptide comprising an amino acid sequence having at        least the percent sequence identity set forth in Table 1 to an        amino acid sequence selected from the group consisting of        sequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9,        10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,        26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41,        42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57,        58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73,        74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,        90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,        105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117,        118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130,        131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,        144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,        157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,        170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,        183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195,        196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208,        209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221,        222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234,        235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247,        248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260,        261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273,        274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286,        287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299,        300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312,        313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,        326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,        339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,        352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,        365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377,        378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390,        391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403,        404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416,        417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429,        430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442,        443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455,        456, 457, and/or 458.

2. The polypeptide of embodiment 1, wherein said polypeptide comprisesthe amino acid sequence set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, and/or 458.

3. A composition comprising the polypeptide of embodiments 1 or 2.

4. The polypeptide of embodiment 2, further comprising heterologousamino acid sequences.

5. A recombinant nucleic acid molecule that encodes the polypeptide ofembodiment 1, wherein said recombinant nucleic acid molecule is not thenaturally occurring sequence encoding said polypeptide.

6. The recombinant nucleic acid of embodiment 5, wherein said nucleicacid molecule is a synthetic sequence that has been designed forexpression in a plant.

7. The recombinant nucleic acid molecule of embodiment 6, wherein saidnucleic acid molecule is operably linked to a promoter capable ofdirecting expression in a plant cell.

8. The recombinant nucleic acid molecule of embodiment 5, wherein saidnucleic acid molecule is operably linked to a promoter capable ofdirecting expression in a bacteria.

9. A host cell that contains the recombinant nucleic acid molecule ofembodiment 8.

10. The host cell of embodiment 9, wherein said host cell is a bacterialhost cell.

11. A DNA construct comprising a promoter that drives expression in aplant cell operably linked to a recombinant nucleic acid moleculecomprising

-   -   (a) a nucleotide sequence that encodes a polypeptide comprising        the amino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5,        6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,        23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,        39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,        55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70,        71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,        87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,        102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,        115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,        128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,        141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153,        154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,        167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,        180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,        193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205,        206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218,        219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,        232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,        245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,        258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270,        271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,        284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,        297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,        310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,        323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,        336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,        349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,        362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,        375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,        388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400,        401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,        414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,        427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439,        440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452,        453, 454, 455, 456, 457, and/or 458; or,    -   (b) a nucleotide sequence that encodes a polypeptide comprising        an amino acid sequence having at least the percent sequence        identity set forth in Table 1 to an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID NOs:        1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,        19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34,        35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,        51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,        67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82,        83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,        99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111,        112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,        125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,        138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,        151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163,        164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176,        177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,        190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,        203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215,        216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228,        229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,        242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,        255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,        268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,        281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293,        294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,        307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319,        320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,        333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345,        346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358,        359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,        372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,        385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397,        398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410,        411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,        424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,        437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,        450, 451, 452, 453, 454, 455, 456, 457, and/or 458.

12. The DNA construct of embodiment 11, wherein said nucleotide sequenceis a synthetic DNA sequence that has been designed for expression in aplant.

13. A vector comprising the DNA construct of embodiment 11.

14. A host cell that contains the DNA construct of any one ofembodiments 11-13.

15. The host cell of embodiment 14, wherein the host cell is a plantcell.

16. A transgenic plant comprising the host cell of embodiment 15.

17. A composition comprising the host cell of embodiment 10.

18. The composition of embodiment 17, wherein said composition isselected from the group consisting of a powder, dust, pellet, granule,spray, emulsion, colloid, and solution.

19. The composition of embodiment 17, wherein said composition comprisesfrom about 1% to about 99% by weight of said polypeptide.

20. A method for controlling a pest population comprising contactingsaid population with a pesticidal-effective amount of the composition ofembodiment 17.

21. A method for killing a pest population comprising contacting saidpopulation with a pesticidal-effective amount of the composition ofembodiment 17.

22. A method for producing a polypeptide with pesticidal activity,comprising culturing the host cell of embodiment 9 under conditions inwhich the nucleic acid molecule encoding the polypeptide is expressed.

23. A plant having stably incorporated into its genome a DNA constructcomprising a nucleotide sequence that encodes a protein havingpesticidal activity, wherein said nucleotide sequence comprise

-   -   (a) a nucleotide sequence that encodes a polypeptide comprising        the amino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5,        6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,        23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,        39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,        55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70,        71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,        87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,        102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,        115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,        128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,        141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153,        154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,        167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,        180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,        193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205,        206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218,        219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,        232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,        245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,        258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270,        271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,        284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,        297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,        310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,        323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,        336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,        349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,        362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,        375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,        388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400,        401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,        414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,        427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439,        440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452,        453, 454, 455, 456, 457, and/or 458; or,    -   (b) a nucleotide sequence that encodes a polypeptide comprising        an amino acid sequence having at least the percent sequence        identity set forth in Table 1 to an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID NOs:        1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,        19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34,        35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,        51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,        67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82,        83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,        99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111,        112, 113, 114, 115, 116, 117, 118, 119,120, 121, 122, 123, 124,        125, 126, 127, 128, 129,130, 131, 132, 133, 134, 135, 136, 137,        138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,        151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163,        164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176,        177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,        190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,        203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215,        216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228,        229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,        242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,        255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,        268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,        281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293,        294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,        307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319,        320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,        333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345,        346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358,        359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,        372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,        385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397,        398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410,        411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,        424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,        437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,        450, 451, 452, 453, 454, 455, 456, 457, and/or 458.

24. A transgenic seed of the plant of embodiment 23.

25. A method for protecting a plant from an insect pest, comprisingexpressing in a plant or cell thereof a nucleotide sequence that encodesa pesticidal polypeptide, wherein said nucleotide sequence comprising

-   -   (a) a nucleotide sequence that encodes a polypeptide comprising        the amino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5,        6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,        23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,        39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,        55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70,        71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,        87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,        102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,        115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,        128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,        141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153,        154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,        167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,        180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,        193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205,        206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218,        219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,        232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,        245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,        258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270,        271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,        284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,        297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,        310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,        323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,        336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,        349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,        362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,        375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,        388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400,        401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,        414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,        427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439,        440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452,        453, 454, 455, 456, 457, and/or 458; or,    -   (b) a nucleotide sequence that encodes a polypeptide comprising        an amino acid sequence having at least the percent sequence        identity set forth in Table 1 to an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID NOs:        1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,        19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34,        35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,        51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,        67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82,        83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,        99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111,        112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,        125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,        138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,        151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163,        164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176,        177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,        190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,        203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215,        216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228,        229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,        242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,        255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,        268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,        281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293,        294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,        307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319,        320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,        333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345,        346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358,        359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,        372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,        385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397,        398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410,        411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,        424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,        437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,        450, 451, 452, 453, 454, 455, 456, 457, and/or 458.

26. The method of embodiment 25, wherein said plant produces apesticidal polypeptide having pesticidal against a lepidopteran orcoleopteran pest.

27. A method for increasing yield in a plant comprising growing in afield a plant or seed thereof having stably incorporated into its genomea DNA construct comprising a promoter that drives expression in a plantoperably linked to a nucleotide sequence that encodes a pesticidalpolypeptide, wherein said nucleotide sequence comprises

-   -   (a) a nucleotide sequence that encodes a polypeptide comprising        the amino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5,        6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,        23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,        39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,        55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70,        71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,        87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,        102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,        115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,        128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,        141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153,        154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,        167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,        180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,        193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205,        206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218,        219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,        232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,        245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,        258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270,        271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,        284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,        297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,        310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,        323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,        336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,        349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,        362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,        375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,        388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400,        401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,        414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,        427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439,        440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452,        453, 454, 455, 456, 457, and/or 458; or,    -   (b) a nucleotide sequence that encodes a polypeptide comprising        an amino acid sequence having at least the percent sequence        identity set forth in Table 1 to an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID NOs:        1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,        19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34,        35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,        51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,        67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82,        83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,        99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111,        112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,        125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,        138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,        151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163,        164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176,        177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,        190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,        203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215,        216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228,        229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,        242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,        255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,        268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,        281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293,        294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,        307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319,        320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,        333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345,        346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358,        359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,        372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,        385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397,        398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410,        411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,        424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,        437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,        450, 451, 452, 453, 454, 455, 456, 457, and/or 458.

28. A method of obtaining a polynucleotide that encodes an improvedpolypeptide comprising pesticidal activity is provided, wherein theimproved polypeptide has at least one improved property over any one ofSEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71,72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105,106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119,120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133,134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217,218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245,246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259,260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273,274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287,288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301,302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315,316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329,330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343,344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385,386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399,400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427,428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441,442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455,456, 457, and/or 458 comprising:

-   -   (a) recombining a plurality of parental polynucleotides        comprising SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13,        14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,        30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,        46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61,        62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77,        78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93,        94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107,        108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120,        121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133,        134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146,        147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159,        160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172,        173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185,        186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,        199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,        212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,        225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237,        238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250,        251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263,        264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276,        277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289,        290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302,        303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315,        316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328,        329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341,        342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354,        355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367,        368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,        381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393,        394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404,405, 406,        407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419,        420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432,        433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445,        446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457,        and/or 458 or an active variant or fragment thereof to produce a        library of recombinant polynucleotides encoding recombinant        pesticidal polypeptides;    -   (b) screening the library to identify a recombinant        polynucleotide that encodes an improved recombinant pesticidal        polypeptide that has an enhanced property improved over the        parental polynucleotide;    -   (c) recovering the recombinant polynucleotide that encodes the        improved recombinant pesticidal polypeptide identified in (b);        and,    -   (d) repeating steps (a), (b) and (c) using the recombinant        polynucleotide recovered in step (c) as one of the plurality of        parental polynucleotides in repeated step (a).

The following examples are offered by way of illustration and not by wayof limitation.

EXPERIMENTAL Example 1: Discovery of Novel Genes by Sequencing and DNAAnalysis

Microbial cultures were grown in liquid culture in standard laboratorymedia. Cultures were grown to saturation (16 to 24 hours) before DNApreparation. DNA was extracted from bacterial cells by detergent lysis,followed by binding to a silica matrix and washing with an ethanolbuffer. Purified DNA was eluted from the silica matrix with a mildlyalkaline aqueous buffer.

DNA for sequencing was tested for purity and concentration byspectrophotometry. Sequencing libraries were prepared using the NexteraXT library preparation kit according to the manufacturer's protocol.Sequence data was generated on a HiSeq 2000 according to the IlluminaHiSeq 2000 System User Guide protocol.

Sequencing reads were assembled into draft genomes using the CLC BioAssembly Cell software package. Following assembly, gene calls were madeby several methods and resulting gene sequences were interrogated toidentify novel homologs of pesticidal genes. Novel genes were identifiedby BLAST, by domain composition, and by pairwise alignment versus atarget set of pesticidal genes. A summary of such sequences is set forthin Table 1.

Genes identified in the homology search were amplified from bacterialDNA by PCR and cloned into bacterial expression vectors containing fusedin-frame purification tags. Cloned genes were expressed in E. coli andpurified by column chromatography. Purified proteins were assessed ininsect diet bioassay studies to identify active proteins.

Example 2. Heterologous Expression in E. coli

Each open reading frame set forth in Table 3 is cloned into an E. coliexpression vector containing a maltose binding protein (pMBP). Theexpression vector is transformed into BL21*RIPL. An LB culturesupplemented with carbenicillin is inoculated with a single colony andgrown overnight at 37 degrees C. using 0.5% of the overnight culture, afresh culture is inoculated and grown to logarithmic phase at 37 degreesC. The culture is induced using 250 mM IPTG for 18 hours at 16 degreesC. The cells are pelleted and resuspended in 10 mM Tris pH7.4 and 150 mMNaCl supplemented with protease inhibitors. The protein expression isevaluated by SDS-PAGE.

Example 3. Pesticidal Activity Against Coleopteran and Lepidoptera

Protein Expression: Each sequence set forth in Table 3 is expressed inE. coli as described in Example 2. 400 mL of LB is inoculated and grownto an OD600 of 0.6. The culture is induced with 0.25 mM IPTG overnightat 16 C. The cells are spun down and the cell pellet is resuspended in 5mL of buffer. The resuspension is sonicated for 2 min on ice.

Bioassay: Fall army worm (FAW), corn ear worm (CEW), European corn borer(ECB) southwestern corn borer (SWCB) and diamond backed moth (DBM or Px)eggs are purchased from a commercial insectary (Benzon Research Inc.,Carlisle, Pa.). The FAW, CEW, ECB and BCW eggs are incubated to thepoint that eclosion would occur within 12 hrs of the assay setup. SWCBand DBM are introduced to the assay as neonate larvae. Assays arecarried out in 24-well trays containing multispecies lepidopteran diet(SOUTHLAND PRODUCTS INCORPORATED, Lake Village, Ark.). Samples of thesonicated lysate are applied to the surface of the diet (diet overlay)and allowed to evaporate and soak into the diet. For CEW, FAW, BCW, ECBand SWCB, a 125 μl of sonicated lysate is added to the diet surface anddried. For DBM, 50 μl of a 1:2 dilution of sonicated lysate was added tothe diet surface. The bioassay plates are sealed with a plate sealingfilm vented with pin holes. The plates are incubated at 26 C at 65% RHon a 16:8 day:night cycle in a Percival for 5 days. The assays areassessed for level of mortality, growth inhibition and feedinginhibition.

For the western corn rootworm bioassay, the protein construct/lysate isevaluated in an insect bioassay by dispensing 60 μl volume on the topsurface of diet in well/s of 24-well plate (Cellstar, 24-well, GreinerBio One) and allowed to dry. Each well contains 500 μl diet (Marrone etal., 1985). Fifteen to twenty neonate larvae are introduced in each wellusing a fine tip paint brush and the plate is covered with membrane(Viewseal, Greiner Bio One). The bioassay is stored at ambienttemperature and scored for mortality, and/or growth/feeding inhibitionat day 4.

For Colorado Potato Beetle (CPB) a cork bore size No. 8 leaf disk isexcised from potato leaf and is dipped in the protein construct/lysateuntil thoroughly wet and placed on top of filter disk (Millipore, glassfiber filter, 13 mm). Sixty μl dH₂O is added to each filter disk andplaced in each well of 24-well plate (Cellstar, 24-well, Greiner BioOne). The leaf disk is allowed to dry and five to seven first instarlarvae are introduced in each well using a fine tip paint brush. Theplate is covered with a membrane (Viewseal, Greiner Bio One) and a smallhole is punctured in each well of the membrane. The construct isevaluated with four replicates, and scored for mortality and leaf damageon day 3.

Example 4. Pesticidal Activity Against Hemipteran

Protein Expression: Each of the sequences set forth in Table 3 isexpressed in E. coli as described in Example 2. 400 mL of LB isinoculated and grown to an OD600 of 0.6. The culture is induced with0.25 mM IPTG overnight at 16 C. The cells are spun down and the cellpellet is re-suspended in 5 mL of buffer. The resuspension is sonicatedfor 2 min on ice.

Second instar SGSB are obtained from a commercial insectary (BenzonResearch Inc., Carlisle, Pa.). A 50% v/v ratio of sonicated lysatesample to 20% sucrose is employed in the bioassay. Stretched parafilm isused as a feeding membrane to expose the SGSB to the diet/samplemixture. The plates are incubated at 25 C:21 C, 16:8 day:night cycle at65% RH for 5 days.

Mortality is scored for each sample.

Example 5. Transformation of Soybean

DNA constructs comprising each of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113,114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197,198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225,226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239,240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281,282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295,296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323,324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337,338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365,366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379,380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393,394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407,408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421,422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435,436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,450, 451, 452, 453, 454, 455, 456, 457, and/or 458 or active variants orfragments thereof operably linked to a promoter active in a plant arecloned into transformation vectors and introduced into Agrobacterium asdescribed in U.S. Provisional Application No. 62/094,782, filed Dec. 19,2015, herein incorporated by reference in its entirety.

Four days prior to inoculation, several loops of Agrobacterium arestreaked to a fresh plate of YEP* medium supplemented with theappropriate antibiotics** (spectinomycin, chloramphenicol andkanamycin). Bacteria are grown for two days in the dark at 28 C. Aftertwo days, several loops of bacteria are transferred to 3 ml of YEPliquid medium with antibiotics in a 125 ml Erlenmeyer flask. Flasks areplaced on a rotary shaker at 250 RPM at 28 C overnight. One day beforeinoculation, 2-3 ml of the overnight culture were transferred to 125 mlof YEP with antibiotics in a 500 ml Erlenmeyer flask. Flasks are placedon a rotary shaker at 250 RPM at 28 C overnight.

Prior to inoculation, the OD of the bacterial culture is checked at OD620. An OD of 0.8-1.0 indicates that the culture is in log phase. Theculture is centrifuged at 4000 RPM for 10 minutes in Oakridge tubes. Thesupernatant is discarded and the pellet is re-suspended in a volume ofSoybean Infection Medium (SI) to achieve the desired OD. The culturesare held with periodic mixing until needed for inoculation.

Two or three days prior to inoculation, soybean seeds are surfacesterilized using chlorine gas. In a fume hood, a petri dish with seedsis place in a bell jar with the lid off. 1.75 ml of 12 N HCl is slowlyadded to 100 ml of bleach in a 250 ml Erlenmeyer flask inside the belljar. The lid is immediately placed on top of the bell jar. Seeds areallowed to sterilize for 14-16 hours (overnight). The top is removedfrom the bell jar and the lid of the petri dish is replaced. The petridish with the surface sterilized is then opened in a laminar flow foraround 30 minutes to disperse any remaining chlorine gas.

Seeds are imbibed with either sterile DI water or soybean infectionmedium (SI) for 1-2 days. Twenty to 30 seeds are covered with liquid ina 100×25 mm petri dish and incubated in the dark at 24 C. Afterimbibition, non-germinating seeds are discarded.

Cotyledonary explants are processed on a sterile paper plate withsterile filter paper dampened using SI medium employing the methods ofU.S. Pat. No. 7,473,822, herein incorporated by reference.

Typically, 16-20 cotyledons are inoculated per treatment. The SI mediumused for holding the explants is discarded and replaced with 25 ml ofAgrobacterium culture (OD 620=0.8-20). After all explants are submerged,the inoculation is carried out for 30 minutes with periodic swirling ofthe dish. After 30 minutes, the Agrobacterium culture is removed.

Co-cultivation plates is prepared by overlaying one piece of sterilepaper onto Soybean Co-cultivation Medium (SCC). Without blotting, theinoculated cotyledons is cultured adaxial side down on the filter paper.Around 20 explants can be cultured on each plate. The plates are sealedwith Parafilm and cultured at 24 C and around 120 umoles m-2s-1 (in aPercival incubator) for 4-5 days.

After co-cultivation, the cotyledons are washed 3 times in 25 ml ofSoybean Wash Medium with 200 mg/l of cefotaxime and timentin. Thecotyledons are blotted on sterile filter paper and then transferred toSoybean Shoot Induction Medium (SSI). The nodal end of the explant isdepressed slightly into the medium with distal end kept above thesurface at about 45 deg. No more than 10 explants are cultured on eachplate. The plates are wrapped with Micropore tape and cultured in thePercival at 24 C and around 120 umoles m-2s-1.

The explants are transferred to fresh SSI medium after 14 days. Emergingshoots from the shoot apex and cotyledonary node are discarded. Shootinduction is continued for another 14 days under the same conditions.

After 4 weeks of shoot induction, the cotyledon is separated from thenodal end and a parallel cut is made underneath the area of shootinduction (shoot pad). The area of the parallel cut is placed on SoybeanShoot Elongation Medium (SSE) and the explants cultured in the Percivalat 24 C and around 120 umoles m-2s-1. This step is repeated every twoweeks for up to 8 weeks as long as shoots continue to elongate.

When shoots reach a length of 2-3 cm, they are transferred to SoybeanRooting Medium (SR) in a Plantcon vessel and incubated under the sameconditions for 2 weeks or until roots reach a length of around 3-4 cm.After this, plants are transferred to soil.

Note, all media mentioned for soybean transformation are found in Paz etal. (2010) Agrobacterium-mediated transformation of soybean and recoveryof transgenic soybean plants; Plant Transformation Facility of IowaState University, which is herein incorporated by reference in itsentirety. (See, the website located atagron-www.agron.iastate.edu/ptf/protocol/Soybean.pdf.)

Example 6. Transformation of Maize

Maize ears are best collected 8-12 days after pollination. Embryos areisolated from the ears, and those embryos 0.8-1.5 mm in size arepreferred for use in transformation. Embryos are plated scutellumside-up on a suitable incubation media, such as DN62A5S media (3.98 g/LN6 Salts; 1 mL/L (of 1000.times. Stock) N6 Vitamins; 800 mg/LL-Asparagine; 100 mg/L Myo-inositol; 1.4 g/L L-Proline; 100 mg/LCasamino acids; 50 g/L sucrose; 1 mL/L (of 1 mg/mL Stock) 2,4-D).However, media and salts other than DN62A5S are suitable and are knownin the art. Embryos are incubated overnight at 25 degree C. in the dark.However, it is not necessary per se to incubate the embryos overnight.

The resulting explants are transferred to mesh squares (30-40 perplate), transferred onto osmotic media for about 30-45 minutes, thentransferred to a beaming plate (see, for example, PCT Publication No.WO/0138514 and U.S. Pat. No. 5,240,842). DNA constructs designed toexpress the GRG proteins of the present invention in plant cells areaccelerated into plant tissue using an aerosol beam accelerator, usingconditions essentially as described in PCT Publication No. WO/0138514.After beaming, embryos are incubated for about 30 min on osmotic media,and placed onto incubation media overnight at 25 degree C. in the dark.To avoid unduly damaging beamed explants, they are incubated for atleast 24 hours prior to transfer to recovery media. Embryos are thenspread onto recovery period media, for about 5 days, 25 degree C. in thedark, then transferred to a selection media. Explants are incubated inselection media for up to eight weeks, depending on the nature andcharacteristics of the particular selection utilized. After theselection period, the resulting callus is transferred to embryomaturation media, until the formation of mature somatic embryos isobserved. The resulting mature somatic embryos are then placed under lowlight, and the process of regeneration is initiated by methods known inthe art. The resulting shoots are allowed to root on rooting media, andthe resulting plants are transferred to nursery pots and propagated astransgenic plants.

Example 7. Pesticidal Activity Against Nematodes Heterodera Glycine's(Soybean Cyst Nematode) In-Vitro Assay.

Soybean Cyst Nematodes are dispensed into a 96 well assay plate with atotal volume of 100 uls and 100 J2 per well. The protein of interest asset forth in any one of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, and/or 458 is dispensed into thewells and held at room temperature for assessment. Finally the 96 wellplate containing the SCN J2 is analyzed for motility. Data is reportedas % inhibition as compared to the controls. Hits are defined as greateror equal to 70% inhibition.

Heterodera Glycine's (Soybean Cyst Nematode) On-Plant Assay

Soybean plants expressing one or more of SEQ ID NO: 1-458 are generatedas described elsewhere herein. A 3-week-old soybean cutting isinoculated with 5000 SCN eggs per plant. This infection is held for 70days and then harvested for counting of SCN cyst that has developed onthe plant. Data is reported as % inhibition as compared to the controls.Hits are defined as greater or equal to 90% inhibition.

Meloidogyne incognita (Root-Knot Nematode) In-Vitro Assay

Root-Knot Nematodes are dispensed into a 96 well assay plate with atotal volume of 100 uls and 100 J2 per well. The protein of interestcomprising any one of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129,130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157,158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171,172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185,186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199,200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213,214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255,256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269,270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297,298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311,312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339,340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353,354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367,368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381,382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395,396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409,410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437,438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451,452, 453, 454, 455, 456, 457, and/or 458 is dispensed into the wells andheld at room temperature for assessment. Finally the 96 well platecontaining the RKN J2 is analyzed for motility. Data is reported as %inhibition as compared to the controls. Hits are defined as greater orequal to 70% inhibition.

Meloidogyne incognita (Root-Knot Nematode) On-Plant Assay

Soybean plants expressing one or more of SEQ ID NO: 1-458 are generatedas described elsewhere herein. A 3-week-old soybean is inoculated with5000 RKN eggs per plant. This infection is held for 70 days and thenharvested for counting of RKN eggs that have developed in the plant.Data is reported as % inhibition as compared to the controls. Hits aredefined as greater or equal to 90% inhibition.

Example 8. Additional Assays for Pesticidal Activity

The various polypeptides set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, and/or 458 can be tested toact as a pesticide upon a pest in a number of ways. One such method isto perform a feeding assay. In such a feeding assay, one exposes thepest to a sample containing either compounds to be tested or controlsamples. Often this is performed by placing the material to be tested,or a suitable dilution of such material, onto a material that the pestwill ingest, such as an artificial diet. The material to be tested maybe composed of a liquid, solid, or slurry. The material to be tested maybe placed upon the surface and then allowed to dry. Alternatively, thematerial to be tested may be mixed with a molten artificial diet, andthen dispensed into the assay chamber. The assay chamber may be, forexample, a cup, a dish, or a well of a microtiter plate.

Assays for sucking pests (for example aphids) may involve separating thetest material from the insect by a partition, ideally a portion that canbe pierced by the sucking mouth parts of the sucking insect, to allowingestion of the test material. Often the test material is mixed with afeeding stimulant, such as sucrose, to promote ingestion of the testcompound.

Other types of assays can include microinjection of the test materialinto the mouth, or gut of the pest, as well as development of transgenicplants, followed by test of the ability of the pest to feed upon thetransgenic plant. Plant testing may involve isolation of the plant partsnormally consumed, for example, small cages attached to a leaf, orisolation of entire plants in cages containing insects.

Other methods and approaches to assay pests are known in the art, andcan be found, for example in Robertson and Preisler, eds. (1992)Pesticide bioassays with arthropods, CRC, Boca Raton, Fla.Alternatively, assays are commonly described in the journals ArthropodManagement Tests and Journal of Economic Entomology or by discussionwith members of the Entomological Society of America (ESA). Any one ofSEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71,72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105,106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119,120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133,134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217,218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245,246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259,260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273,274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287,288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301,302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315,316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329,330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343,344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385,386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399,400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427,428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441,442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455,456, 457, and/or 458 can be expressed and employed in an assay as setforth in Examples 3 and 4, herein.

Example 9. Pesticidal Activity Against Coleopteran and Lepidoptera

Protein Expression: Each sequence set forth in Table 3 was expressed inE. coli as described in Example 2. 400 mL of LB was inoculated and grownto an OD600 of 0.6. The culture was induced with 0.25 mM IPTG overnightat 16° C. The cells were spun down and the cell pellet was resuspendedin 5 mL of buffer. The resuspension was sonicated for 2 min on ice.

Bioassay: Fall army worm (FAW), corn ear worm (CEW), European corn borer(ECB) southwestern corn borer (SWCB) and diamond backed moth (DBM or Px)eggs were purchased from a commercial insectary (Benzon Research Inc.,Carlisle, Pa.). The FAW, CEW, ECB and BCW eggs were incubated to thepoint that eclosion would occur within 12 hrs of the assay setup. SWCBand DBM were introduced to the assay as neonate larvae. Assays werecarried out in 24-well trays containing multispecies lepidopteran diet(Southland Products Inc., Lake Village, Ark.). Samples of the sonicatedlysate were applied to the surface of the diet (diet overlay) andallowed to evaporate and soak into the diet. For CEW, FAW, BCW, ECB andSWCB, a 125 μl of sonicated lysate was added to the diet surface anddried. For DBM, 50 μl of a 1:2 dilution of sonicated lysate was added tothe diet surface. The bioassay plates were sealed with a plate sealingfilm vented with pin holes. The plates were incubated at 26° C. at 65%relative humidity (RH) on a 16:8 day:night cycle in a Percival for 5days. The assays were assessed for level of mortality, growth inhibitionand feeding inhibition.

For the western corn rootworm (WCR) bioassay, the proteinconstruct/lysate was evaluated in an insect bioassay by dispensing 60 μlvolume on the top surface of diet in well/s of 24-well plate (Cellstar,24-well, Greiner Bio One) and allowed to dry. Each well contained 500 μldiet (Marrone et al., 1985). Fifteen to twenty neonate larvae wereintroduced in each well using a fine tip paint brush and the plate wascovered with membrane (Viewseal, Greiner Bio One). The bioassay wasstored at ambient temperature and scored for mortality, and/orgrowth/feeding inhibition at day 4.

For Colorado Potato Beetle (CPB) a cork bore size No. 8 leaf disk wasexcised from potato leaf and was dipped in the protein construct/lysateuntil thoroughly wet and placed on top of filter disk (Millipore, glassfiber filter, 13 mm). 60 μl dH2O was added to each filter disk andplaced in each well of 24-well plate (Cellstar, 24-well, Greiner BioOne). The leaf disk was allowed to dry and five to seven first instarlarvae were introduced in each well using a fine tip paint brush. Theplate was covered with a membrane (Viewseal, Greiner Bio One) and asmall hole was punctured in each well of the membrane. The construct wasevaluated with four replicates, and scored for mortality and leaf damageon day 3.

Table 3 provides a summary of pesticidal activity against coleopteranand lepidoptera of the various sequences. Table code: “−” indicates noactivity seen; “+” indicates pesticidal activity; “NT” indicates nottested; “S” indicates stunt; “SS” indicates slight stunt; “HM” indicateshigh mortality; “LF” indicates low feeding; “M” indicates mortality.

TABLE 3 Summary of Pesticidal Activity against Coleopteran andLepidoptera. WCR Mortality APG Seq ID NO: FAW CEW BCW ECB SWCB CPB (%)APG01506.0 Seq ID 22 − NT NT NT NT NT − APG07655.0 Seq ID 246 − NT NT NTNT NT − APG06690.0 Seq ID 209 − NT NT NT NT NT − APG01309.0 Seq ID 9 −NT NT NT NT NT − APG08631.1 Seq ID 302 M, S NT NT NT NT NT − APG08589.0Seq ID 300 M, S NT NT NT NT NT − APG03379.0 Seq ID 84 − NT NT NT NT NT −APG00954.0 Seq ID 2 − NT NT NT NT NT − APG04076.0 Seq ID 114 SS − − NTNT − − APG04076.1 Seq ID 115 SS − SS NT NT − − APG06431.1 Seq ID 203 −NT NT NT NT NT − APG07860.1 Seq ID 267 − SS − NT NT − − APG03080.0 SeqID 76 − NT NT NT NT NT − APG02912.1 Seq ID 69 HM, S NT NT NT NT NT −APG04460.1 Seq ID 130 M, S NT NT NT NT NT − APG01900.0 Seq ID 131 M, SNT NT NT NT NT − APG08919.1 Seq ID 316 − NT NT NT NT NT − APG01718.1 SeqID 32 − NT NT NT NT NT − APG07857.0 Seq ID 265 SS NT NT NT NT NT −APG02215.1 Seq ID 49 M, S NT NT NT NT NT − APG02445.1 Seq ID 58 M, S NTNT NT NT NT − APG03604.0 Seq ID 89 SS SS SS NT NT + 96 APG03249.1 Seq ID80 M, S NT NT NT NT NT − APG03299.0 Seq ID 81 − − − NT NT − − APG09484.1Seq ID 328 − NT NT NT NT NT − APG06176.1 Seq ID 192 − NT NT NT NT NT −APG02782.1 Seq ID 64 − NT NT NT NT NT − APG02782.2 Seq ID 63 − NT NT NTNT NT − APG04013.1 Seq ID 113 − NT NT NT NT NT − APG04597.1 Seq ID 135HM, S NT NT NT NT NT − APG03726.1 Seq ID 96 M, S NT NT NT NT NT −APG03015.1 Seq ID 71 − NT NT NT NT NT − APG02638.0 Seq ID 61 − NT NT NTNT NT − APG04720.1 Seq ID 142 S S S NT NT − − APG08088.1 Seq ID 279 SSNT NT NT NT − − APG09682.0 Seq ID 339 − NT NT NT NT NT − APG05328.0 SeqID 161 − NT NT NT NT NT − APG07961.0 Seq ID 272 − NT NT NT NT NT −APG04725.1 Seq ID 144 HM, S NT NT NT NT NT − APG05045.1 Seq ID 158 − SSSS NT NT − − APG09589.0 Seq ID 336 − NT NT NT NT NT − APG02798.1 Seq ID66 SS NT NT NT NT NT − APG01937.0 Seq ID 45 − NT NT NT NT NT −APG04477.1 Seq ID 133 − NT NT NT NT NT − APG08139.0 Seq ID 281 − NT NTNT NT NT − APG06428.0 Seq ID 200 − NT NT NT NT NT − APG02204.0 Seq ID201 M, S NT NT NT NT NT − APG03786.1 Seq ID 103 − NT NT NT NT NT −APG09877.0 Seq ID 342 M, S NT NT NT NT NT − APG07803.1 Seq ID 262 SS NTNT NT NT NT − APG04682.1 Seq ID 140 SS NT NT NT NT NT − APG04332.1 SeqID 119 M, S NT NT NT NT NT − APG05930.1 Seq ID 185 − NT NT NT NT NT −APG07037.1 Seq ID 235 − NT NT NT NT NT − APG05025.1 Seq ID 153 SS NT NTNT NT NT − APG08108.0 Seq ID 280 HM, S NT NT NT NT NT − APG06938.0 SeqID 223 − NT NT NT NT NT − APG00929.0 Seq ID 1 − NT NT NT NT NT −APG04176.1 Seq ID 117 − NT NT NT NT NT − APG05715.1 Seq ID 178 − NT NTNT NT NT − APG08817.1 Seq ID 311 − NT NT NT NT NT − APG08817.2 Seq ID312 HM, S NT NT NT NT NT − APG02168.0 Seq ID 47 − NT NT NT NT NT −APG09232.1 Seq ID 321 − NT NT NT NT NT − APG03732.1 Seq ID 98 − NT NT NTNT NT − APG03227.1 Seq ID 78 − NT NT NT NT NT − APG02477.1 Seq ID 60 M,S NT NT NT NT NT − APG07748.2 Seq ID 253 − NT NT NT NT NT − APG09630.1Seq ID 338 − NT NT NT NT NT − APG08396.2 Seq ID 296 − NT NT NT NT NT −APG07518.1 Seq ID 242 SS NT NT NT NT NT − APG01849.2 Seq ID 41 S NT NTNT NT NT − APG08350.1 Seq ID 293 SS NT NT NT NT NT − APG05658.1 Seq ID176 M, S NT NT NT NT NT − APG09084.0 Seq ID 319 M, S NT NT NT NT NT −APG03053.1 Seq ID 73 M, S NT NT NT NT NT − APG07396.0 Seq ID 240 HM, SNT NT NT NT NT − APG04431.1 Seq ID 128 − NT NT NT NT NT − APG05804.0 SeqID 179 HM, S NT NT NT NT NT − APG08230.1 Seq ID 286 M, S − − NT NT + −APG06051.0 Seq ID 188 − NT NT NT NT NT − APG07818.1 Seq ID 264 HM, S NTNT NT NT NT − APG06921.0 Seq ID 222 − NT NT NT NT NT − APG01459.1 Seq ID19 − NT NT NT NT NT − APG08657.1 Seq ID 304 M, S NT NT NT NT NT −APG07731.1 Seq ID 251 M, S NT NT NT NT NT − APG03848.1 Seq ID 105 − NTNT NT NT NT − APG05370.1 Seq ID 163 − NT NT NT NT NT − APG01780.1 Seq ID37 − NT NT NT NT NT − APG01915.1 Seq ID 44 M, S NT NT NT NT NT −APG06253.1 Seq ID 194 HM, S NT NT NT NT NT − APG04350.1 Seq ID 121 − NTNT NT NT NT − APG05810.1 Seq ID 181 − NT NT NT NT NT − APG06995.0 Seq ID228 M, S NT NT NT NT NT − APG01742.1 Seq ID 34 − NT NT NT NT NT −APG01644.1 Seq ID 28 − NT NT NT NT NT − APG05506.0 Seq ID 167 HM, S NTNT NT NT NT − APG07783.1 Seq ID 256 SS NT NT NT NT NT − APG05615.1 SeqID 169 − NT NT NT NT NT − APG01790.1 Seq ID 39 − NT NT NT NT NT −APG07559.1 Seq ID 245 − − SS NT NT − − APG07559.2 Seq ID 244 − − SS NTNT − − APG02293.1 Seq ID 53 − NT NT NT NT NT − APG05651.1 Seq ID 172 −NT NT NT NT NT − APG01772.0 Seq ID 35 SS NT NT NT NT NT − APG01399.1 SeqID 17 − NT NT NT NT NT − APG07036.1 Seq ID 233 M, S NT NT NT NT NT +APG03072.1 Seq ID 75 − NT NT NT NT NT − APG06372.2 Seq ID 198 SS NT NTNT NT NT − APG05978.1 Seq ID 187 − NT NT NT NT NT − APG01239.0 Seq ID227 − NT NT NT NT NT − APG06942.1 Seq ID 226 − NT NT NT NT NT −APG06942.2 Seq ID 225 − NT NT NT NT NT − APG07100.0 Seq ID 238 − NT NTNT NT NT − APG07100.1 Seq ID 239 − NT NT NT NT NT − APG07870.1 Seq ID271 − NT NT NT NT NT − APG07870.2 Seq ID 270 − NT NT NT NT NT −APG03519.1 Seq ID 86 − NT NT NT NT NT − APG03519.2 Seq ID 87 − NT NT NTNT NT − APG09936.1 Seq ID 344 − NT NT NT NT NT − APG06650.0 Seq ID 207 −NT NT NT NT NT − APG06650.1 Seq ID 208 M, S NT NT NT NT NT − APG01387.1Seq ID 14 − NT NT NT NT NT − APG01387.2 Seq ID 15 − NT NT NT NT NT −APG01238.0 Seq ID 213 − NT NT NT NT NT − APG06739.1 Seq ID 212 − NT NTNT NT NT − APG06739.2 Seq ID 211 − NT NT NT NT NT − APG09573.1 Seq ID335 − NT NT NT NT NT − APG09573.2 Seq ID 334 − NT NT NT NT NT −APG08174.2 Seq ID 283 − NT NT NT NT NT − APG02809.0 Seq ID 67 M, SS NTNT NT NT NT − APG03746.2 Seq ID 100 − NT NT NT NT NT − APG08039.1 Seq ID275 − NT NT NT NT NT − APG02291.0 Seq ID 51 − NT NT NT NT NT −APG01522.1 Seq ID 26 − NT NT NT NT NT − APG09413.0 Seq ID 326 − NT NT NTNT NT − APG04978.0 Seq ID 151 M, SS NT NT NT NT NT − APG04418.2 Seq ID125 − NT NT NT NT NT − APG03569.0 Seq ID 88 − NT NT NT NT NT −APG06630.2 Seq ID 205 − NT NT NT NT NT − APG08931.1 Seq ID 318 − NT NTNT NT NT − APG03901.2 Seq ID 107 SS NT NT NT NT NT − APG08851.0 Seq ID323 SS NT NT NT NT NT − APG09234.0 Seq ID 322 SS NT NT NT NT NT −APG01474.1 Seq ID 21 − NT NT NT NT NT − APG08525.1 Seq ID 299 M, SS NTNT NT NT NT − APG04598.0 Seq ID 136 M, S NT NT NT NT NT − APG08693.1 SeqID 309 − NT NT NT NT NT − APG02408.2 Seq ID 55 SS NT NT NT NT NT −APG06161.1 Seq ID 190 SS NT NT NT NT NT − APG01044.1 Seq ID 4 − NT NT NTNT NT − APG07866.0 Seq ID 268 − NT NT NT NT NT − APG03309.1 Seq ID 83 SSNT NT NT NT NT − APG08461.0 Seq ID 297 SS − − NT NT − − APG01996.0 SeqID 46 − NT NT NT NT NT − APG07983.0 Seq ID 273 − NT NT NT NT NT −APG08278.2 Seq ID 289 SS NT NT NT NT NT − APG07787.0 Seq ID 257 SS NT NTNT NT NT − APG06768.0 Seq ID 214 SS NT NT NT NT NT − APG08898.1 Seq ID314 − NT NT NT NT NT − APG06880.1 Seq ID 218 SS NT NT NT NT NT −APG01087.1 Seq ID 6 − NT NT NT NT NT − APG06912.2 Seq ID 221 SS NT NT NTNT NT − APG05384.2 Seq ID 166 SS NT NT NT NT NT − APG05653.1 Seq ID 174SS NT NT NT NT NT − APG04640.1 Seq ID 138 M, SS NT NT NT NT NT −APG04804.1 Seq ID 146 SS NT NT NT NT NT − APG09296.1 Seq ID 325 − NT NTNT NT NT − APG06364.1 Seq ID 196 − NT NT NT NT NT − APG05034.2 Seq ID155 SS NT NT NT NT NT − APG01517.1 Seq ID 24 SS NT NT NT NT NT −APG06784.1 Seq ID 216 SS NT NT NT NT NT − APG05619.0 Seq ID 170 SS NT NTNT NT NT − APG08665.2 Seq ID 306 − NT NT NT NT NT − APG09543.1 Seq ID330 SS NT NT NT NT NT − APG09947.1 Seq ID 346 SS NT NT NT NT NT −APG03699.1 Seq ID 91 − NT NT NT NT NT − APG09864.1 Seq ID 341 − NT NT NTNT NT − APG07058.1 Seq ID 237 − NT NT NT NT NT − APG04807.1 Seq ID 148 −NT NT NT NT NT − APG08307.1 Seq ID 291 − NT NT NT NT NT − APG03995.1 SeqID 354 − NT NT NT NT NT − APG03000.0 Seq ID 352 SS NT NT NT NT NT −APG08065.1 Seq ID 277 − NT NT NT NT NT − APG01231.1 Seq ID 8 − NT NT NTNT NT − APG03722.2 Seq ID 94 − NT NT NT NT NT − APG07016.2 Seq ID 231 −NT NT NT NT NT − APG01329.2 Seq ID 11 HM, S NT NT NT NT NT − APG01676.1Seq ID 30 − NT NT NT NT NT − APG09545.1 Seq ID 332 SS NT NT NT NT NT −APG05924.1 Seq ID 183 SS NT NT NT NT NT − APG09984.0 Seq ID 347 M, SS NTNT NT NT NT − APG04931.1 Seq ID 150 S NT NT NT NT NT − APG04365.1 Seq ID123 − NT NT NT NT NT − APG07790.2 Seq ID 259 SS NT NT NT NT NT −APG07672.2 Seq ID 249 SS NT NT NT NT NT − APG05084.1 Seq ID 160 SS NT NTNT NT NT − APG03947.2 Seq ID 110 M, SS NT NT NT NT NT − APG06291.1 SeqID 360 − NT NT NT NT NT − APG05497.1 Seq ID 358 M, SS NT NT NT NT NT −APG09231.0 Seq ID 367 − NT NT NT NT NT − APG02585.1 Seq ID 351 − NT NTNT NT NT − APG04196.1 Seq ID 356 − NT NT NT NT NT − APG00905.1 Seq ID349 SS NT NT NT NT NT − APG06371.1 Seq ID 362 − NT NT NT NT NT −APG07648.1 Seq ID 366 SS NT NT NT NT NT − APG07577.1 Seq ID 364 SS NT NTNT NT NT − APG07724.1 Seq ID 422 − NT NT NT NT NT − APG01809.1 Seq ID376 − NT NT NT NT NT − APG04458.1 Seq ID 403 M, SS NT NT NT NT NT −APG09824.1 Seq ID 430 − NT NT NT NT NT − APG04251.1 Seq ID 401 − NT NTNT NT NT − APG05226.1 Seq ID 410 SS NT NT NT NT NT − APG06330.2 Seq ID415 SS NT NT NT NT NT − APG07936.1 Seq ID 424 − NT NT NT NT NT −APG08381.1 Seq ID 426 SS NT NT NT NT NT − APG00923.2 Seq ID 370 − NT NTNT NT NT − APG02362.2 Seq ID 382 M, SS NT NT NT NT NT − APG03337.1 SeqID 393 − NT NT NT NT NT − APG00941.1 Seq ID 372 − NT NT NT NT NT −APG03438.1 Seq ID 398 − NT NT NT NT NT − APG07160.2 Seq ID 420 − NT NTNT NT NT − APG03297.2 Seq ID 391 − NT NT NT NT NT − APG04650.2 Seq ID406 − NT NT NT NT NT − APG05337.1 Seq ID 412 − NT NT NT NT NT −APG02686.2 Seq ID 388 − NT NT NT NT NT − APG02421.2 Seq ID 385 − NT NTNT NT NT − APG02194.2 Seq ID 379 − NT NT NT NT NT − APG09755.1 Seq ID428 − NT NT NT NT NT − APG06841.1 Seq ID 417 − NT NT NT NT NT −APG03352.1 Seq ID 395 − NT NT NT NT NT − APG03422.0 Seq ID 396 − NT NTNT NT NT − APG04806.1 Seq ID 408 − NT NT NT NT NT − APG01202.0 Seq ID373 NT NT NT NT NT NT NT APG03891.0 Seq ID 399 − NT NT NT NT NT −APG03861.2 Seq ID 442 SS NT NT NT NT NT − APG07911.1 Seq ID 455 − NT NTNT NT NT − APG06587.1 Seq ID 453 SS NT NT NT NT NT − APG04374.3 Seq ID446 SS NT NT NT NT NT − APG01515.1 Seq ID 432 − NT NT NT NT NT −APG05852.1 Seq ID 450 − NT NT NT NT NT − APG08051.2 Seq ID 458 − NT NTNT NT NT −

Example 10. Pesticidal Activity Against Hemipteran

Protein Expression: Each of the sequences set forth in Table 4 wasexpressed in E. coli as described in Example 2. 400 mL of LB wasinoculated and grown to an OD600 of 0.6. The culture was induced with0.25 mM IPTG overnight at 16° C. The cells were spun down and the cellpellet was re-suspended in 5 mL of buffer. The resuspension wassonicated for 2 min on ice.

Second instar southern green stinkbug (SGSB) were obtained from acommercial insectary (Benzon Research Inc., Carlisle, Pa.). A 50% v/vratio of sonicated lysate sample to 20% sucrose was employed in thebioassay. Stretched parafilm was used as a feeding membrane to exposethe SGSB to the diet/sample mixture. The plates were incubated at 25°C.: 21° C., 16:8 day:night cycle at 65% RH for 5 days.

Mortality was scored for each sample. The results are set forth in Table4. A dashed line indicates no mortality was detected. The proteinslisted in Table 4 showed from about 10% to about 100% mortality 25%mortality or 50% mortality (as indicated) against southern greenstinkbug (1 stinkbug out of 4 died). The negative controls (empty vectorexpressed binding domain and buffer only) both showed no mortality (0stinkbugs out of 4).

TABLE 4 Summary of Pesticidal Activity against Hemipteran APG Seq IDTested against SGSB (%) APG01506.0 Seq ID 22 40 APG07655.0 Seq ID 246 20APG06690.0 Seq ID 209 30 APG01309.0 Seq ID 9 30 APG08631.1 Seq ID 302 30APG08589.0 Seq ID 300 10 APG03379.0 Seq ID 84 0 APG00954.0 Seq ID 2 20APG04076.0 Seq ID 114 0 APG04076.1 Seq ID 115 0 APG06431.1 Seq ID 203 30APG07860.1 Seq ID 267 20 APG03080.0 Seq ID 76 20 APG02912.1 Seq ID 69 40APG04460.1 Seq ID 130 40 APG01900.0 Seq ID 131 10 APG08919.1 Seq ID 3160 APG01718.1 Seq ID 32 30 APG07857.0 Seq ID 265 40 APG02215.1 Seq ID 4920 APG02445.1 Seq ID 58 30 APG03604.0 Seq ID 89 100 APG03249.1 Seq ID 800 APG03299.0 Seq ID 81 25 APG09484.1 Seq ID 328 20 APG06176.1 Seq ID 19220 APG02782.1 Seq ID 64 30 APG02782.2 Seq ID 63 30 APG04013.1 Seq ID 11320 APG04597.1 Seq ID 135 30 APG03726.1 Seq ID 96 60 APG03015.1 Seq ID 7140 APG02638.0 Seq ID 61 30 APG04720.1 Seq ID 142 20 APG08088.1 Seq ID279 30 APG09682.0 Seq ID 339 30 APG05328.0 Seq ID 161 30 APG07961.0 SeqID 272 20 APG04725.1 Seq ID 144 30 APG05045.1 Seq ID 158 40 APG09589.0Seq ID 336 0 APG02798.1 Seq ID 66 30 APG01937.0 Seq ID 45 0 APG04477.1Seq ID 133 40 APG08139.0 Seq ID 281 90 APG06428.0 Seq ID 200 0APG02204.0 Seq ID 201 40 APG03786.1 Seq ID 103 10 APG09877.0 Seq ID 34210 APG07803.1 Seq ID 262 0 APG04682.1 Seq ID 140 10 APG04332.1 Seq ID119 20 APG05930.1 Seq ID 185 30 APG07037.1 Seq ID 235 20 APG05025.1 SeqID 153 0 APG08108.0 Seq ID 280 20 APG06938.0 Seq ID 223 0 APG00929.0 SeqID 1 40 APG04176.1 Seq ID 117 40 APG05715.1 Seq ID 178 40 APG08817.1 SeqID 311 30 APG08817.2 Seq ID 312 0 APG02168.0 Seq ID 47 10 APG09232.1 SeqID 321 20 APG03732.1 Seq ID 98 30 APG03227.1 Seq ID 78 20 APG02477.1 SeqID 60 20 APG07748.2 Seq ID 253 50 APG09630.1 Seq ID 338 20 APG08396.2Seq ID 296 50 APG07518.1 Seq ID 242 0 APG01849.2 Seq ID 41 10 APG08350.1Seq ID 293 0 APG05658.1 Seq ID 176 30 APG09084.0 Seq ID 319 20APG03053.1 Seq ID 73 30 APG07396.0 Seq ID 240 30 APG04431.1 Seq ID 12850 APG05804.0 Seq ID 179 10 APG08230.1 Seq ID 286 40 APG06051.0 Seq ID188 20 APG07818.1 Seq ID 264 10 APG06921.0 Seq ID 222 40 APG01459.1 SeqID 19 10 APG08657.1 Seq ID 304 10 APG07731.1 Seq ID 251 20 APG03848.1Seq ID 105 60 APG05370.1 Seq ID 163 30 APG01780.1 Seq ID 37 20APG01915.1 Seq ID 44 40 APG06253.1 Seq ID 194 60 APG04350.1 Seq ID 12110 APG05810.1 Seq ID 181 20 APG06995.0 Seq ID 228 10 APG01742.1 Seq ID34 60 APG01644.1 Seq ID 28 60 APG05506.0 Seq ID 167 40 APG07783.1 Seq ID256 30 APG05615.1 Seq ID 169 30 APG01790.1 Seq ID 39 0 APG07559.1 Seq ID245 75 APG07559.2 Seq ID 244 25 APG02293.1 Seq ID 53 40 APG05651.1 SeqID 172 30 APG01772.0 Seq ID 35 50 APG01399.1 Seq ID 17 10 APG07036.1 SeqID 233 20 APG03072.1 Seq ID 75 40 APG06372.2 Seq ID 198 30 APG05978.1Seq ID 187 50 APG01239.0 Seq ID 227 30 APG06942.1 Seq ID 226 0APG06942.2 Seq ID 225 10 APG07100.0 Seq ID 238 20 APG07100.1 Seq ID 23940 APG07870.1 Seq ID 271 40 APG07870.2 Seq ID 270 20 APG03519.1 Seq ID86 30 APG03519.2 Seq ID 87 40 APG09936.1 Seq ID 344 50 APG06650.0 Seq ID207 50 APG06650.1 Seq ID 208 70 APG01387.1 Seq ID 14 0 APG01387.2 Seq ID15 10 APG01238.0 Seq ID 213 50 APG06739.1 Seq ID 212 20 APG06739.2 SeqID 211 40 APG09573.1 Seq ID 335 50 APG09573.2 Seq ID 334 30 APG08174.2Seq ID 283 50 APG02809.0 Seq ID 67 40 APG03746.2 Seq ID 100 30APG08039.1 Seq ID 275 30 APG02291.0 Seq ID 51 30 APG01522.1 Seq ID 26 30APG09413.0 Seq ID 326 20 APG04978.0 Seq ID 151 50 APG04418.2 Seq ID 12530 APG03569.0 Seq ID 88 30 APG06630.2 Seq ID 205 10 APG08931.1 Seq ID318 30 APG03901.2 Seq ID 107 20 APG08851.0 Seq ID 323 50 APG09234.0 SeqID 322 40 APG01474.1 Seq ID 21 70 APG08525.1 Seq ID 299 30 APG04598.0Seq ID 136 50 APG08693.1 Seq ID 309 30 APG02408.2 Seq ID 55 40APG06161.1 Seq ID 190 40 APG01044.1 Seq ID 4 20 APG07866.0 Seq ID 268 40APG03309.1 Seq ID 83 0 APG08461.0 Seq ID 297 40 APG01996.0 Seq ID 46 60APG07983.0 Seq ID 273 40 APG08278.2 Seq ID 289 20 APG07787.0 Seq ID 25730 APG06768.0 Seq ID 214 60 APG08898.1 Seq ID 314 40 APG06880.1 Seq ID218 20 APG01087.1 Seq ID 6 10 APG06912.2 Seq ID 221 40 APG05384.2 Seq ID166 30 APG05653.1 Seq ID 174 20 APG04640.1 Seq ID 138 0 APG04804.1 SeqID 146 40 APG09296.1 Seq ID 325 30 APG06364.1 Seq ID 196 40 APG05034.2Seq ID 155 20 APG01517.1 Seq ID 24 30 APG06784.1 Seq ID 216 50APG05619.0 Seq ID 170 10 APG08665.2 Seq ID 306 50 APG09543.1 Seq ID 33060 APG09947.1 Seq ID 346 30 APG03699.1 Seq ID 91 40 APG09864.1 Seq ID341 50 APG07058.1 Seq ID 237 50 APG04807.1 Seq ID 148 10 APG08307.1 SeqID 291 30 APG03995.1 Seq ID 354 40 APG03000.0 Seq ID 352 50 APG08065.1Seq ID 277 30 APG01231.1 Seq ID 8 0 APG03722.2 Seq ID 94 30 APG07016.2Seq ID 231 10 APG01329.2 Seq ID 11 40 APG01676.1 Seq ID 30 60 APG09545.1Seq ID 332 10 APG05924.1 Seq ID 183 20 APG09984.0 Seq ID 347 10APG04931.1 Seq ID 150 50 APG04365.1 Seq ID 123 30 APG07790.2 Seq ID 25940 APG07672.2 Seq ID 249 40 APG05084.1 Seq ID 160 30 APG03947.2 Seq ID110 10 APG06291.1 Seq ID 360 20 APG05497.1 Seq ID 358 50 APG09231.0 SeqID 367 30 APG02585.1 Seq ID 351 20 APG04196.1 Seq ID 356 0 APG00905.1Seq ID 349 0 APG06371.1 Seq ID 362 30 APG07648.1 Seq ID 366 0 APG07577.1Seq ID 364 20 APG07724.1 Seq ID 422 10 APG01809.1 Seq ID 376 30APG04458.1 Seq ID 403 40 APG09824.1 Seq ID 430 40 APG04251.1 Seq ID 40140 APG05226.1 Seq ID 410 100 APG06330.2 Seq ID 415 60 APG07936.1 Seq ID424 40 APG08381.1 Seq ID 426 40 APG00923.2 Seq ID 370 70 APG02362.2 SeqID 382 40 APG03337.1 Seq ID 393 10 APG00941.1 Seq ID 372 30 APG03438.1Seq ID 398 50 APG07160.2 Seq ID 420 20 APG03297.2 Seq ID 391 60APG04650.2 Seq ID 406 40 APG05337.1 Seq ID 412 30 APG02686.2 Seq ID 38840 APG02421.2 Seq ID 385 20 APG02194.2 Seq ID 379 30 APG09755.1 Seq ID428 40 APG06841.1 Seq ID 417 30 APG03352.1 Seq ID 395 0 APG03422.0 SeqID 396 0 APG04806.1 Seq ID 408 0 APG01202.0 Seq ID 373 50 APG03891.0 SeqID 399 50 APG03861.2 Seq ID 442 50 APG07911.1 Seq ID 455 30 APG06587.1Seq ID 453 0 APG04374.3 Seq ID 446 10 APG01515.1 Seq ID 432 NTAPG05852.1 Seq ID 450 NT APG08051.2 Seq ID 458 NT

Example 11. Pesticidal Activity Against Soybean Aphid

Protein Expression: Each sequence set forth in Table 6 (or an activevariant or fragment thereof) was expressed in E. coli as described inExample 2. 400 mL of LB was inoculated and grown to an OD600 of 0.6. Theculture was induced with 0.25 mM IPTG overnight at 16° C. The cells werespun down and the cell pellet was resuspended in 5 mL of buffer. Theresuspension was sonicated for 2 min on ice.

Soybean aphids (SBA) were obtained from Michigan State University. Sixadult aphids were added to each well of a 24 well plate. Purifiedproteins were provided in liquid artificial diet at a rate of 25% (50 ulprotein, 150 ul artificial diet), and were sealed with an artificialmembrane through which the aphids are able to feed. The plates were heldin an incubator at 26° C., 60% RH, 16:8 day:night cycle for 5 days.Mortality, feeding, and reproduction were scored for each sample on days3, 4, and 5 post-treatment. Mortality was calculated as percent dead ofthe original 6 adult aphids.

Feed and reproduction activities were assigned a score on a 0-3 pointscale with 0 being no feeding or reproduction and 3 being high feedingor reproduction. Feeding was measured as the amount of honeydew (liquidexcretions produced by aphids) that collected in each well. Reproductionwas the number of live immature aphids that were observed in each well.Mortality, feeding, and reproduction data were assessed using a combinedscoring system to establish cutoff levels for activity. The combinedscore was calculated as:

Combined Score=(Feeding+Reproduction+Mortality Score)/3, where MortalityScore=3−(3×% Mortality/100). The results are set forth in Table 6. “+”indicates pesticidal activity.

TABLE 5 Cut-offs used to rate individual wells as active or not for eachobservational day post-introduction of aphids to assay wells. A well isdeemed active if measure ≤ cut-off. Cut-off used to designate activityActivity Measure Day 3 Day 4 Day 5 Combined Score 2 1.5 1

TABLE 6 Summary of Pesticidal Activity against soybean aphid APG Seq IDTested against SBA APG03604.0 Seq ID 89 + APG04597.1 Seq ID 135 +APG03726.1 Seq ID 96 + APG05226.1 Seq ID 410 +SEQ ID NOs: 1, 2, 4, 6, 8, 9, 11, 14, 15, 17, 19, 21, 22, 24, 26, 28,30, 32, 34, 35, 37, 39, 41, 44, 45, 46, 47, 49, 51, 53, 55, 58, 60, 61,63, 64, 66, 67, 69, 71, 73, 75, 76, 80, 81, 83, 84, 86, 87, 88, 91, 94,98, 100, 103, 105, 107, 110, 113, 117, 119, 121, 123, 125, 128, 130,131, 133, 136, 138, 140, 142, 144, 146, 148, 150, 151, 153, 155, 158,160, 161, 163, 166, 167, 169, 170, 172, 174, 176, 178, 179, 181, 183,185, 187, 188, 190, 192, 194, 196, 198, 200, 201, 203, 205, 207, 208,209, 211, 212, 213, 214, 216, 218, 221, 222, 223, 225, 226, 227, 228,231, 233, 235, 237, 238, 239, 240, 242, 244, 245, 246, 249, 251, 253,256, 257, 259, 262, 264, 265, 267, 268, 270, 271, 272, 273, 275, 277,279, 280, 281, 283, 286, 289, 291, 293, 296, 297, 299, 300, 302, 304,306, 309, 311, 312, 314, 316, 318, 319, 321, 322, 323, 325, 326, 328,330, 332, 334, 335, 336, 338, 339, 341, 342, 344, 346, 347, 349, 351,352, 354, 356, 358, 360, 362, 364, 366, 367, 370, 372, 373, 376, 379,382, 385, 388, 391, 393, 395, 396, 398, 399, 401, 403, 406, 408, 412,415, 417, 420, 422, 424, 426, 428, 430, 442, 446, 453, and 455 weretested and did not have activity in this experiment.

All publications and patent applications mentioned in the specificationare indicative of the level of skill of those skilled in the art towhich this invention pertains. All publications and patent applicationsare herein incorporated by reference to the same extent as if eachindividual publication or patent application was specifically andindividually indicated to be incorporated by reference.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity ofunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the appended claims.

1. An isolated polypeptide comprising (a) an amino acid sequence havingat least 90% sequence identity to the amino acid sequence set forth inSEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71,72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105,106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119,120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133,134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217,218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245,246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259,260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273,274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287,288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301,302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315,316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329,330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343,344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385,386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399,400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427,428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441,442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455,456, 457, or 458, wherein the polypeptide has pesticidal activity; (b)an amino acid sequence having at least 95% sequence identity to theamino acid sequence set forth in SEO ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113,114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197,198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225,226, 227, 228, 229, 230, 231, 232, 233, 234, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, or 312, wherein the polypeptide has pesticidal activity; or (c) theamino acid sequence set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113,114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197,198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225,226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239,240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281,282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295,296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323,324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337,338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365,366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379,380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393,394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407,408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421,422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435,436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,450, 451, 452, 453, 454, 455, 456, 457, or 458; and wherein thepolypeptide further comprises a heterologous amino acid sequencechemically linked to the polypeptide. 2-3. (canceled)
 4. A recombinantnucleic acid molecule encoding a polypeptide comprising: (a) an aminoacid sequence having at least 90% sequence identity to the amino acidsequence set forth in SEQ ID No: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129,130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157,158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171,172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185,186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199,200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213,214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255,256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269,270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297,298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311,312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339,340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353,354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367,368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381,382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395,396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409,410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437,438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451,452, 453, 454, 455, 456, 457, or 458, wherein the polypeptide haspesticidal activity; (b) an amino acid sequence having at least 95%sequence identity to the amino acid sequence set forth in SEO ID NO: 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21,22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39,40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57,58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75,76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108,109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122,123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164,165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178,179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206,207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220,221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234,235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248,249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262,263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276,277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290,291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304,305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346,347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360,361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388,389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402,403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416,417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430,431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444,445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, or 458,wherein the polypeptide has pesticidal activity; or (c) the amino acidsequence set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129,130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157,158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171,172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185,186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199,200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213,214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255,256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269,270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297,298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311,312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339,340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353,354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367,368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381,382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395,396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409,410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437,438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451,452, 453, 454, 455, 456, 457, or
 458. 5. The recombinant nucleic acidmolecule of claim 4, wherein said recombinant nucleic acid molecule is asynthetic sequence designed for expression in a plant. 6.-7. (canceled)8. A host cell comprising the recombinant nucleic acid molecule of claim4.
 9. The host cell of claim 8, wherein said host cell is a bacterialhost cell or a plant cell.
 10. A DNA construct comprising a heterologouspromoter operably linked to the recombinant nucleic acid molecule ofclaim
 4. 11. The DNA construct of claim 10, wherein said nucleic acidmolecule is a synthetic DNA sequence that has been designed forexpression in a plant.
 12. A vector comprising the DNA construct ofclaim
 11. 13. A host cell comprising the vector of claim
 12. 14.-20.(canceled)
 21. A method for producing a polypeptide with pesticidalactivity, comprising culturing the host cell of claim 8 under conditionsin which the nucleic acid molecule encoding the polypeptide isexpressed.
 22. A plant having stably incorporated into its genome theDNA construct of claim
 10. 23. A transgenic seed of the plant of claim22, wherein said seed has stably incorporated into its genome the DNAconstruct.
 24. A method for protecting a plant from an insect pest,comprising expressing in a plant or cell thereof the nucleic acidmolecule of claim
 4. 25. The method of claim 24, wherein said plantproduces a pesticidal polypeptide having pesticidal activity against alepidopteran, a hemipteran, and/or coleopteran pest.
 26. A method forincreasing yield in a plant comprising growing in a field a plant orseed thereof having stably incorporated into its genome the DNAconstruct of claim
 10. 27. (canceled)
 28. The DNA construct of claim 10,wherein the heterologous promoter drives expression in a plant cell or abacterial cell.
 29. A formulation comprising a polypeptide, wherein thepolypeptide comprises: (a) an amino acid sequence having at least 90%sequence identity to the amino acid sequence set forth in SEQ ID NO: 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21,22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39,40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57,58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75,76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108,109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122,123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164,165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178,179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206,207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220,221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234,235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248,249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262,263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276,277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290,291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304,305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346,347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360,361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388,389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402,403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416,417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430,431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444,445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, or 458,wherein the polypeptide has pesticidal activity; (b) an amino acidsequence having at least 95% sequence identity to the amino acidsequence set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129,130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157,158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171,172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185,186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199,200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213,214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255,256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269,270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297,298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311,312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339,340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353,354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367,368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381,382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395,396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409,410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437,438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451,452, 453, 454, 455, 456, 457, or 458, wherein the polypeptide haspesticidal activity; or (c) the amino acid sequence set forth in SEQ IDNO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19,20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37,38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55,56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73,74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107,108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121,122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135,136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149,150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163,164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177,178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191,192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205,206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219,220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233,234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247,248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261,262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275,276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289,290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303,304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317,318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331,332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345,346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359,360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373,374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401,402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415,416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429,430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443,444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, or458.
 30. The formulation of claim 29, wherein said formulation isselected from the group consisting of a powder, dust, pellet, wettablegranule, wettable powder, spray, emulsion, colloid, and solution.
 31. Amethod for controlling a pest population comprising contacting saidpopulation with a pesticidal-effective amount of the formulation ofclaim
 29. 32. The plant of claim 22, wherein said pesticidal activitycontrols a lepidopteran pest, a hemipteran pest, or a coleopteran pest.33. The plant of claim 22, wherein the plant is a monocot.
 34. The plantof claim 22, wherein the plant is a dicot.
 35. The plant of claim 33,wherein the plant is corn, sorghum, wheat, rice, sugarcane, barley,oats, rye, millet, coconut, pineapple or banana.
 36. The plant of claim34, wherein the plant is sunflower, tomato, crucifers, peppers, potato,cotton, soybean, sugarbeet, tobacco, oilseed rape, sweet potato,alfalfa, safflower, peanuts, cassava, coffee, cocoa, cucumber, lettuce,olive, peas, or tea.
 37. The method of claim 24, wherein protecting saidplant comprises controlling insect pest damage to said plant.